LPS-induced mucin expression in human sinus mucosa can be attenuated by hCLCA inhibitors

Journal of Endotoxin Research

Volumn 13, Issue 2, 2007, Pages 109-116

  Hauber, Hans Peter ^a   Goldmann, Torsten ^a   Vollmer, Ekkehard ^a   Wollenberg, Barbara ^b   Hung, Hsiao Ling ^c   Levitt, Roy C ^c   Zabel, Peter ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

^b UNIVERSITY HOSPITAL SCHLESWIG HOLSTEIN   (Germany)

^c Genaera Corporation   (United States)

Author keywords

Airway; hCLCA1; LPS; Mucus; Niflumic acid

Indexed keywords

CALCIUM ION; CHLORIDE CHANNEL; CHLORIDE CHANNEL BLOCKING AGENT; LIPOPOLYSACCHARIDE; MESSENGER RNA; MSI 2216; MUCIN; MUCIN 5AC; NIFLUMIC ACID; UNCLASSIFIED DRUG; CLCA1 PROTEIN, HUMAN;

ADULT; AIRWAY; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG EFFECT; EOSINOPHIL; EX VIVO STUDY; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INFLAMMATORY CELL; LYMPHOCYTE; MALE; MONOCYTE; NEUTROPHIL; NOSE MUCOSA; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RESPIRATORY TRACT MUCOSA; UPREGULATION; BIOSYNTHESIS; CELL LINE; DRUG ANTAGONISM; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; INFLAMMATION; INNATE IMMUNITY; METABOLISM; MIDDLE AGED; MUCUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE CULTURE TECHNIQUE;

BACTERIA (MICROORGANISMS);

ADULT; CELL LINE; CHLORIDE CHANNELS; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HUMANS; IMMUNITY, NATURAL; IMMUNOHISTOCHEMISTRY; INFLAMMATION; LIPOPOLYSACCHARIDES; MALE; MIDDLE AGED; MUCINS; MUCUS; NIFLUMIC ACID; RESPIRATORY MUCOSA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TISSUE CULTURE TECHNIQUES;

EID: 34547562985     PISSN: 09680519     EISSN: None     Source Type: Journal    
DOI: 10.1177/0968051907079168     Document Type: Article

References (20)

1. Thorn J. The inflammatory response in humans after inhalation of bacterial endotoxin: A review. Inflamm Res 2001; 50: 254-261.
2. Dohrman A., Miyata S., Gallup M. et al. Mucin gene (MUC 2 and MUC 5AC) upregulation by Gram-positive and Gram-negative bacteria. Biochim Biophys Acta 1998; 1406: 251-259.
3. Yanagihara K., Seiki M., Cheng PW Lipopolysaccharide induces mucus cell metaplasia in mouse lung. Am J Respir Cell Mol Biol 2001; 24: 66-73.
4. Barnes PJ Anti-inflammatory actions of glucocorticoids: Molecular mechanisms. Clin Sci 1998; 94: 557-572.
5. Lu W., Lillehoj EP, Kim KC Effects of dexamethasone on Muc5ac mucin production by primary airway goblet cells. Am J Physiol 2005; 288: L52-L60.
6. Chen Y., Nickola TJ, DiFronzo NL et al. Dexamethasone-mediated repression of MUC5AC gene expression in human lung epithelial cells. Am J Respir Cell Mol Biol 2006; 34: 338-347.
7. Nakano T., Inoue H., Fukuyama S. et al. Niflumic acid suppresses interleukin-13-induced asthma phenotypes. Am J Respir Crit Care Med 2006; 173: 1216-1221.
8. Nakanishi A., Morita S., Iwashita H. et al. Role of gob-5 in mucus overproduction and airway hyperresponsiveness in asthma. Proc Natl Acad Sci USA 2001;98: 5175-5180.
9. Gruber AD, Elble RC, Ji HL, Schreur KD, Fuller CM, Pauli BU Genomic cloning, molecular characterization, and functional analysis of human CLCA1, the first human member of the family of Ca2+-activated Cl- channel proteins. Genomics 1998; 54: 200-214.
10. Zhou Y., Dong Q., Louahed J. et al. Characterization of a calcium-activated chloride channel as a shared target of Th2 cytokine pathways and its potential involvement in asthma. Am J Respir Cell Mol Biol 2001; 25: 486-491.
11. Hauber HP, Daigneault P., Frenkiel S. et al. Niflumic acid and MSI-2216 reduce TNF-alpha-induced mucin expression in human airway mucosa. J Allergy Clin Immunol 2005; 115: 266-271.
12. Wagner JG, Harkema JR, Levitt RC, Holroyd KJ Attenuation of endotoxin-induced mucus hypersecretion and airway inflammation by MSI-2216, a calcium-activated chloride channel inhibitor (abstract). Am J Respir Crit Care Med 2003; 167: A466.
13. Dandurand RJ, Wang CG, Laberge S., Martin JG, Eidelman DH In vitro allergic bronchoconstriction in the brown Norway rat. Am J Respir Crit Care Med 1994; 149: 1499-1505.
14. Minshall E., Wang CG, Dandurand R., Eidelman D. Heterogeneity of responsiveness of individual airways in cultured lung explants. Can J Physiol Pharmacol 1997; 75: 911-916.
15. Goldmann T., Vollmer E., Gerdes J. What's cooking? Detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval. Am J Pathol 2003; 163: 2638-2640.
16. Hovenberg HW, Davies JR, Herrmann A., Linden CJ, Carlstedt I. MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions. Glycoconj J 1996; 13: 839-847.
17. Long AJ, Sypek JP, Askew R. et al. Gob-5 contributes to goblet cell hyperplasia and modulates pulmonary tissue inflammation. Am J Respir Cell Mol Biol 2006; 35: 357-365.
18. Gibson A., Lewis AP, Affleck K. et al. hCLCA1 and mCLCA3 are secreted non-integral membrane proteins and therefore are not ion channels. J Biol Chem 2005; 280: 27205-27212.
19. Leverkoehne I., Gruber AD The murine mCLCA3 (alias gob-5) protein is located in the mucin granule membranes of intestinal, respiratory, and uterine goblet cells. J Histochem Cytochem 2002; 50: 829-838.
20. Civelli M., Vigano T., Acerbi D. et al. Modulation of arachidonic acid metabolism by orally administered momiflumate in man. Agents Actions 1991; 33: 233-239.