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0) of RNase A upon adsorption (2.5 kcal/mol for the conjugate of 4 nm particles and 4.5 kcal/mol for the conjugate of 15 nm particles), the relative numbers of interacting charge pairs between one RNase A and one silica particle thus are calculated to be ∼1.8 for the conjugate of 4 nm particles and ∼3.2 for the conjugate of 15 nm particles.
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