In vitro refolding of PEGylated lipase

Journal of Biotechnology

Volumn 131, Issue 2, 2007, Pages 177-179

  Kim, Min Young ^a   Kwon, Jin Sook ^a   Kim, Hyung Jin ^a   Lee, E K ^a  

^a Hanyang University   (South Korea)

Author keywords

Integrated process; PEGylated lipase; PEGylation; Refolding

Indexed keywords

DRUG PRODUCTS PLANTS; POLYETHYLENE GLYCOLS; PROTEINS; PURIFICATION;

INTEGRATED PROCESS; PEGYLATED LIPASE; PEGYLATION; REFOLDING;

ENZYME KINETICS;

MACROGOL; TRIACYLGLYCEROL LIPASE; UREA;

ARTICLE; COVALENT BOND; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING;

BURKHOLDERIA CEPACIA; FEASIBILITY STUDIES; LIPASE; POLYETHYLENE GLYCOLS; PROTEIN DENATURATION; PROTEIN FOLDING;

EID: 34547533094     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.06.016     Document Type: Article

References (17)

1. Bailon P., and Verthold W. Polyethylene glycol-conjugated pharmaceutical proteins. Pharm. Sci., Tech. Today 1 (1998) 352-356
2. Choi W.C., Kim M.Y., Suh C.W., and Lee E.K. Solid-phase refolding of inclusion body protein in a packed and expanded bed adsorption chromatography. Process Biochem. 40 (2005) 1967-1972
3. Conover C.D., Greenwald R.B., Pendri A., and Shum K.L. Camptothecin delivery systems: the utility of amino acid spacers for the conjugation of camptothecin with polyethylene glycol to create prodrugs. Anticancer Drug Des. 14 (1999) 499-506
4. Fee C.J., and Alstine J.M.V. PEG-protein: reaction engineering and separation issues. Chem. Eng. Sci. 61 (2006) 924-939
5. Greenwald R.B., Choe Y.H., McGuire J., and Conover C.D. Effective drug delivery by PEGylated drug conjugates. Adv. Drug Deliv. Rev. 55 (2003) 217-250
6. Hinds K.D., and Kim S.W. Effect of PEG conjugation on insulin properties. Adv. Drug Deliv. Rev. 54 (2002) 505-530
7. Inada Y., Nishimura H., and Takahashi K. Ester synthesis catalyzed by polyethylene glycol-modified lipase in benzene. Biochem. Biophys. Res. Commun. 122 2 (1984) 845-850
8. Kinstler, O.B., Grabriel, N.E., Farrar, C.E., DePrince, R.B., 1994. N-terminally chemically modified protein compositions and methods. US patent No. 5, 824, 784.
9. Kinstler O.B., Brems D.N., Lauren S.L., Paige A.G., Hamburger J.B., and Treuheit M.J. Characterization and stability of N-terminally PEGylated rhG-CSF. Pharm. 13 (1996) 996-1002
10. Kinstler O.B., Molineux G., Treuheit M., Ladd D., and Gegg C. Mono-N-terminal poly(ethylene glycol)-protein conjugates. Adv. Drug Deliv. 54 (2002) 477-485
11. Lee E.K., and Lee J.D. Solid-phase, N-terminus-specific, Mono-PEGylation of Recombinant Interferon alpha-2a: Purification, Characterization, and Bioactivity, Presented at ISPPP 2004 (2004), Aachen, Germany
12. Lesuisse E., Schanck K., and Colson C. Purification and preliminary characterization of the extracellular lipase of Bacillus subtilis 168, an extremely basic pH-tolerant enzyme. Eur. J. Biochem. 216 (1993) 155-160
13. Means G.E., and Feeney R.E. Reductive alkylation of proteins. Anal. Biochem. 224 (1995) 1-16
14. Sorina M.A., Schrimsher J.L., and Chavez M.D. PEGylation of proteins: a structural approach. Biopharm. Int. 19 4 (2006) 34-49
15. Yamamoto Y., Tsutsumi Y., Yoshioka Y., Nishibata T., Kobayashi K., Okamoto T., Mukai Y., Shimizu T., Nakagawa S., Nagata S., and Mayumi T. Site-specific PEGylation of a lysine-deficient TNF-alpha with full bioactivity. Nat. Biotechnol. 21 (2003) 546-552
16. Yohalem D.S., and Lorbeer J.W. Intraspecific metabolic diversity among strains of Burkholderia cepacia isolated from decayed onions, soils, and the clinical environment. Antonie Van Leeuwenhoek 65 2 (1994) 111-131
17. Zalipsky S. Chemistry of polyethylene glycol conjugates with biologically active molecules. Adv. Drug Deliv. 16 (1995) 157-182