Identification of reference genes for qRT-PCR studies of gene expression in giant cells and syncytia induced in Arabidopsis thaliana by Meloidogyne incognita and Heterodera schachtii

Nematology

Volumn 9, Issue 3, 2007, Pages 317-323

  Hofmann, Julia ^a   Grundler, Florian M W ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

18S RNA; Real time PCR; UBP22

Indexed keywords

CYST; GENE; GENE EXPRESSION; HERB; IDENTIFICATION METHOD; INFECTIOUS DISEASE; NEMATODE; PARASITIC DISEASE; POLYMERASE CHAIN REACTION; ROOT;

ARABIDOPSIS; ARABIDOPSIS THALIANA; HETERODERA SCHACHTII; MELOIDOGYNE; MELOIDOGYNE INCOGNITA; MELOIDOGYNE JAVANICA; NEMATODA;

EID: 34547504269     PISSN: 13885545     EISSN: 15685411     Source Type: Journal    
DOI: 10.1163/156854107781352034     Document Type: Article

References (24)

1. DE ALMEIDA ENGLER, J., DE VLEESSCHAUWER, V., BRUSSENS, S., GELENZA, J.L.J., INZÉ, D., VAN MONTAGU, M., ENGLER, G. & GHEYSEN, G. (1999). Molecular markers and cell cycle inhibitor show the importance of cell cycle progression in nematode-induced galls and syncytia. Plant Cell 11, 793-807.
2. DE ALMEIDA ENGLER, J., VAN POUKE, K., KARIM, M., DE GROODT, R., GHEYSEN, G., ENGLER, G. & GHEYSEN, G. (2004). Dynamic cytoskeleton rearrangements in giant cells and syncytia of nematode-infected roots. The Plant Journal 38, 12-26.
3. BAR-OR, C., KAPULNIK, Y. & KOLTAI, H. (2004). Using laser capture microdissection to study gene expression in early stages of giant cells induced by root-knot nematodes. Molecular Plant Pathology 5, 587-592.
4. BUSTIN, S.A. (2000). Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology 25, 169-193.
5. CZECHOWSKI, T., BARI, R.P., STITT, M., SCHEIBLE, W.R. & UDVARDI, M.K. (2004). Real time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. The Plant Journal 38, 366-379.
6. CZECHOWSKI, T., STITT, M., ALTMANN, T., UDVARDI, M.K. & SCHEIBLE, W.-R. (2005). Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiology 139, 5-17.
7. FAVERY, B., CHELYSHEVA, L.A., LEBRIS, M., JAMMES, F., MARMAGNE, A., DE ALMEIDA- ENGLER, J., LECOMTE, P., VAURY, C., ARKOWITZ, R.A. & ABAD, P. (2004). Arabidopsis formin atfh6 is a plasma membrane-associated protein upregulated in giant cells induced by parasitic nematodes. The Plant Cell 16, 2529-2540.
8. GHEYSEN, G. & FENOLL, C. (2002). Gene expression in nematode feeding sites. Annual Review of Phytopathology 40, 191-219.
9. GOLINOWSKI, W., GRUNDLER, F.M.W. & SOBCZAK, M. (1996). Changes in the structure of Arabidopsis thaliana during female development of the plant-parasitic nematode Heterodera schachtii. Protoplasma 194, 103-116.
10. GOVERSE, A., DE ALMEIDA ENGLER, J., VERHEES, J., VAN DER KROL, S., HELDER, J. & GHEYSEN, G. (2000). Cell cycle activation by plant parasitic nematodes. Plant Molecular Biology 43, 747-761.
11. HAMMES, U.Z., SCHACHTMAN, D.P., BERG, R.H., NIELSEN, E., KOCH, W., MCINTYRE, L.M. & TAYLOR, C.G. (2005). Nematode-induced changes of transporter gene expression in Arabidopsis roots. Molecular Plant-Microbe Interactions 18, 1247-1257.
12. HOFMANN, J., WIECZOREK, K., BLÖCHL, A. & GRUNDLER, F.M.W. (2007). Sucrose supply to nematode-induced syncytia depends on the apoplasmic and the symplasmic pathway. Journal of Experimental Botany, in press.
13. HUSSEY, R.S. & GRUNDLER, F.M.W. (1998). Nematode parasitism of plants. In: Perry, R.N. & Wright, D.J. (Eds). The physiology and biochemistry of free-living and plant parasitic nematodes. Wallingford, UK, CABI Publishing, pp. 213-243.
14. JAMMES, F., LECOMTE, P., DE ALMEIDA-ENGLER, J., BITTON, F., MARTIN- MAGNIETTE, M.-L., RENOU, J.P., ABAD, P. & FAVERY, B. (2005). Genome-wide expression profiling of the host response to root-knot nematode infection in Arabidopsis. The Plant Journal 44, 447-458.
15. KARSAI, A., MÜLLER, S., PLATZ, S. & HAUSER, M.-T. (2002). Evaluation of a home-made SYBR Green I reaction mixture for real-time PCR quantification of gene expression. BioTechniques 32, 790-796.
16. KIM, B.-R., NAM, H.-Y., KIM, S.-U., KIM, S.-I. & CHANG, Y.-J. (2003). Normalization of reverse transcription quantitative-PCR with housekeeping genes in rice. Biotechnology Letters 25, 869-1872. LAU, J., CASTELLI, L.A., LIN, E.C.K. & MACAULAY, S.L. (2006). Identification of elongation factor 1α as a potential associated binding partner for Akt2. Molecular and Cellular Biochemistry 286, 17-22.
17. -ddct method. Methods 25, 402-408.
18. NICOT, N., HAUSMAN, J.-F., HOFFMANN, L. & EVERS, D. (2005). Housekeeping gene selection for real-time RTPCR normalization in potato during biotic and abiotic stress. Journal of Experimental Botany 56, 2907-2914.
19. PUTHOFF, D.P., NETTLETON, D., RODERMEL, S.R. & BAUM, T.J. (2003). Arabidopsis gene expression changes during cyst nematode parasitism revealed by statistical analysis of microarray expression profiles. The Plant Journal 33, 911-921.
20. RAMSAY, K., WANG, Z. & JONES, M.G.K. (2005). A broad characterization of the transcriptional profile of the compatible tomato response to the plant parasitic root knot nematode Meloidogyne javanica. European Journal of Plant Pathology 111, 181-192.
21. REID, K.E., OLSSON, N., SCHLOSSER, J., PENG, F. & LUND, S.T. (2006). An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biology 14, 27.
22. SCHMITTGEN, T.D. & ZAKRAJSEK, B.A. (2000). Effect of experimental treatment on houskeeping gene expression: Validation by real-time quantitative RT-PCR. Journal of Biochemical and Biophysical Methods 46, 69-81.
23. SUN, C.-H. & CALLIS, J. (1997). Independent modulation of Arabidopsis thaliana polyubiquitin mRNA in different organs and in response to environmental changes. The Plant Journal 11, 1017-1027.
24. YERGEAU, E., SOMMERVILLE, D.W., MAHEUX, E., VUJANOVIC, V., HAMEL, C., WHALEN, J.K. & ST-ARNAUD, M. (2006). Relationships between Fusarium population structure, soil nutrient status and disease incidence in fieldgrown asparagus. FEMS Microbiology Ecology 58, 394-403.