Molecular cloning and biochemical characterization of a family-9 endoglucanase with an unusual structure from the gliding bacteria Cytophaga hutchinsonii

Applied Biochemistry and Biotechnology

Volumn 141, Issue 1, 2007, Pages 127-138

  Louime, Clifford ^a,c   Abazinge, Michael ^a   Johnson, Elijah ^a   Latinwo, Lekan ^a   Ikediobi, Christopher ^a   Clark, Ann Marie ^b  

^a FLORIDA A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF FLORIDA   (United States)

^c UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Cellulase; Cellulose binding domain; Cytophaga

Indexed keywords

CELLULASE; CELLULOSE-BINDING DOMAIN; CYTOPHAGA; NONPROCESSIVE ENZYMES;

BIOCHEMISTRY; BIOMASS; CLONING; ENZYMES; MOLECULAR DYNAMICS;

BACTERIA;

BACTERIAL ENZYME; BETA 1,4 ENDOGLUCANASE; CARBOXYMETHYLCELLULOSE; CELLULOSE; GLUCAN SYNTHASE; POLYHISTIDINE TAG; POLYPEPTIDE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BINDING SITE; BIOCHEMISTRY; CATALYSIS; CYTOPHAGA; CYTOPHAGA HUTCHINSONII; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; EXPRESSION VECTOR; FILTER; GENE OVEREXPRESSION; GENE STRUCTURE; HYDROLYSIS; MICROBIAL DEGRADATION; MOLECULAR CLONING; MOLECULAR WEIGHT; PAPER; PH; PHOTOTROPHIC, GLIDING, SHEATHED, BUDDING OR APPENDAGED BACTERIA; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; PROTEIN FAMILY; PROTEIN PROCESSING; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; CELLULASE; CLONING, MOLECULAR; CYTOPHAGA; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS;

CYTOPHAGA; CYTOPHAGA HUTCHINSONII; ESCHERICHIA COLI;

EID: 34547495240     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-007-9215-3     Document Type: Article

References (31)

1. Wilson, D.B. and Irwin, D. (1999), Adv. Biochem. Eng. Biotech. 65, 1-21.
2. Lynd, L.R. (1996), Annu. Rev. Energy Environm. 21, 403-465.
3. Leschine, S. B. (1995), Annu. Rev. Microbiol. 49, 399-426.
4. Knowles, J., Lehtovaara, P., and Teeri, T. (1987), Trends Biotechnol. 5, 255-261.
5. Glick, B. R. and Pasternak, J. J. (1989), Biotech. Adv. 7, 361-386.
6. Lynd, L. R., Weimer, P. J., Willem, H. van Zyl, W. H., and Pretorius, I. S. (2002), Microb. Mol. Bio. Rev. 66, 506-577.
7. Sambrook, J., Fritsch, E. F., and Miniatis, T. (1989), in Molecular Cloning - A laboratory Manual, Second ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Dnastar (2002), Lasergene software. www.DNASTAR.com.
9. Clustalw (2002), EBI - European Bioinformatics Institute. www.ebi.ac.uk/clustalw.
10. CodeHop (2002), Consensus Degenerate Hybrid Oligonucleotide Primers - www.bioinformatics.weizmann.ac.il/codehop.html.
11. Qiagen. DNA Cleanup Protocol (2003), www.qiagen.com/Products/DnaCleanup.
12. Genecodes (2002), Sequencher. www.genecodes.com/sequencher.
13. Promega (2003), pGEM -T and pGEM -T Easy Vector Systems Technical Manual, TM042. www.promega.com/tbs/tm042/tm042.pdf.
14. Novagen (2003), pET28 His-Tag Vector system Manual. www.novagen.com.
15. Zhou, W., Irwin, D., Kousen, J. E. and Wilson, D. B. (2004), Biochemistry 43, 9655-63.
16. NIH (National Institute of Health) (2002), National Center for Biotechnology Information. www.ncbi.nlm.nih.gov.
17. PROSITE. Expasy Proteomics Server (2002), www.expasy.org/prosite.
18. Miller, G. L. (1959), Anal. Chem. 31, 426-428.
19. Bradford, M. (1976), Anal. Biochem. 72, 248-252.
20. Lowry, O. H. and Rosenbrough, A. L. (1951), J. Biol. Chem. 193, 265-275.
21. Wilson, D. B. (2004) The Chemical Record 4, 72- 82.
22. CBS - Center for Biological Sequence Analysis (2005), www.cbs.dtu.dk/services/SignalP
23. Rosano, C., Bisso. A., Izzo, G., et al. (2000), J. Mol. Biol. 303, 77-91.
24. Henrissat, B., Claeyssens, M., Tomme, P., Lemesle, L., and Mornon, J. P. (1989), Gene 81, 83-95.
25. Degani, O., Gepstein, S., and Dosoretz, C. (2004), J. Biotechn. 107, 265-273.
26. Lu-Shan, W., Jie, L., Yu-Zhong, Z., Yue, Z., and Pei-Ji, G. (2003), J. Mol. Catal., B Enzym. 24, 27-38.
27. Cavaco-Paulo, A., Morgado, J., Andreaus, J., and Kilburn, D. (1999), Enzyme Microb. Technol. 25, 639-643.
28. Carrard, M. L., Forro, L. M., and Pekker, S. (1996), Synthetic Metals 80, 29-34.
29. Lemos, M. A., Teixeira, M. R. M., Domingues, M., and Gama, F. M. (2003), Enzyme Microb. Technol. 32, 35-40.
30. Linder, M. and Teeri, T. (1997), Proc. Natl. Acad. Sci. USA 93, 12,251-12,255.
31. Kerita, S., Kazuo, S., and Kunio, A. (1996), J. Ferment. Bioeng. 81, 553-556.