Evaluation of porB variable region typing of Neisseria gonorrhoeae using PCR-ELISA in samples collected from men who have sex with men

Journal of Clinical Laboratory Analysis

Volumn 21, Issue 4, 2007, Pages 237-243

  Ling, A E ^a   Bash, M C ^b   Lynn, F ^b   Lister, N A ^c   Zhu, P ^d   Garland, S M ^a,e   Fairley, C K ^c,e   Tabrizi, S N ^a,e  

^a ROYAL WOMEN'S HOSPITAL   (Australia)

^b CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

^c MELBOURNE SEXUAL HEALTH CENTRE   (Australia)

^d CREATV MICROTECH INC   (United States)

^e UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

ELISA; MSM; N. gonorrhoeae; PCR; porB; Typing

Indexed keywords

ANALYTIC METHOD; ARTICLE; BACTERIAL GENE; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC TEST; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; HUMAN; HYBRIDIZATION; MALE; MALE HOMOSEXUAL; NEISSERIA GONORRHOEAE; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEIC ACID AMPLIFICATION; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; PORB GENE; THROAT CULTURE;

BACTERIAL TYPING TECHNIQUES; BASE SEQUENCE; DNA PROBES; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; GONORRHEA; HOMOSEXUALITY, MALE; HUMANS; MALE; MOLECULAR SEQUENCE DATA; NEISSERIA GONORRHOEAE; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; PORINS; VARIATION (GENETICS);

NEISSERIA GONORRHOEAE; NEISSERIA MENINGITIDIS;

EID: 34547422273     PISSN: 08878013     EISSN: 10982825     Source Type: Journal    
DOI: 10.1002/jcla.20173     Document Type: Article

References (33)

1. Lister NA, Fairley CK, Read T, Mijch A. Screening for STIs in individuals with HIV infection. Sex Transm Infect 2002; 78:387-388.
2. Lister NA, Smith A, Tabrizi S, et al. Screening for Neisseria gonorrhoeae and Chlamydia trachomatis in men who have sex with men (MSM) at male-only saunas. Sex Transm Dis 2003;30: 886-889.
3. Sandstrom EG, Knapp JS, Reller LB, Thompson SE, Hook III EW, Holmes KK. Serogrouping of Neisseria gonorrhoeae: correlation of serogroup with disseminated gonococcal infection. Sex Transm Dis 1984;11:77-80.
4. Knapp JS, Holmes KK, Bonin P, Hook EW 3rd. Epidemiology of gonorrhea: distribution and temporal changes in auxotype/serovar classes of Neisseria gonorrhoeae. Sex Transm Dis 1987;14:26-32.
5. Unemo M, Olcen P, Albert J, Fredlund H. Comparison of serologic and genetic porB-based typing of Neisseria gonorrhoeae: consequences for future characterization. J Clin Microbiol 2003; 41:4141-4147.
6. O'Rourke M, Ison CA, Renton AM, Spratt BG. Opa-typing - a high-resolution tool for studying the epidemiology of gonorrhoea. Mol Microbiol 1995;17:865-875.
7. Xia M, Whittington WL, Holmes KK, Plummer FA, Roberts MC. Pulsed-field gel electrophoresis for genomic analysis of Neisseria gonorrhoeae. J Infect Dis 1995;171:455-458.
8. Hobbs MM, Alcorn TM, Davis RH, Fischer W, Thomas JC, Martin I, Ison C, Sparling PF, Cohen MS. Molecular typing of Neisseria gonorrhoeae causing repeated infections: evolution of porin during passage within a community. J Infect Dis 1999;179: 371-381.
9. Viscidi RP, Demma JC, Gu J, Zenilman J. Comparison of sequencing of the por gene and typing of the opa gene for discrimination of Neisseria gonorrhoeae strains from sexual contacts. J Clin Microbiol 2000;38:4430-4438.
10. Howie F, Young H, McMillan A. The diversity of the opa gene in gonococcal isolates from men who have sex with men. Sex Transm Infect 2004;80:286-288.
11. Martin IM, Ison CA, Aanensen DM, Fenton KA, Spratt BG. Rapid sequence-based identification of gonococcal transmission clusters in a large metropolitan area. J Infect Dis 2004;189: 1497-1505
12. Unemo MP, Olcén P, Jonasson OJ, Fredlund H. Molecular typing of Neisseria gonorrhoeae isolates by pyrosequencing of highly polymorphic segments of the porB gene. J Clin Microbiol 2004; 42:2926-2934.
13. Airell A, Lindback E, Ataker F, Pornull KJ, Wretlind B. Verification of clinical samples, positive in AMPLICOR Neisseria gonorrhoeae polymerase chain reaction, by 16S rRNA and gyrA compared with culture. Int J STD AIDS 2005;16:415-419.
14. Lindback E, Gharizadeh B, Ataker F, et al. DNA gyrase gene in Neisseria gonorrhoeae as indicator for resistance to ciprofloxacin and species verification. Int J STD AIDS 2005;16:142-147.
15. Viscidi RP, Demma JC. Genetic diversity of Neisseria gonorrhoeae housekeeping genes. J Clin Microbiol 2003;41:197-204.
16. Douglas JT, Lee MD, Nikaido H. Protein I of N. gonorrhoeae outer membrane is a porin. FEMS Microbiol Lett 1988;12: 305-309.
17. Rudel T, Schmid A, Benz R, Kolb HA, Lang F, Meyer TF. Modulation of Neisseria porin (PorB) by cytosolic ATP/GTP of target cells: parallels between pathogen accommodation and mitochondrial endosymbiosis. Cell 1996;85.391-402.
18. Merz AJ, So M. Interactions of pathogenic Neisseriae with epithelial cell membranes. Annu Rev Cell Dev Biol 2000;16: 423-457.
19. Gorby GL, Ehrhardt AF, Apicella MA, Elkins C. Invasion of human fallopian tube epithelium by Escherichia coli expressing combinations of a gonococcal porin, opacity-associated protein, and chimeric lipo-oligosaccharide. J Infect Dis 2001;184: 460-472.
20. Edwards JL, Brown EJ, Uk-Nham S, Cannon JG, Blake MS, Apicella MA. A co-operative interaction between Neisseria gonorrhoeae and complement receptor 3 mediates infection of primary cervical epithelial cells. Cell Microbiol 2002;4:571-584.
21. Massari P, Ram S, Macleod H, Wetzler LM. The role of porins in neisserial pathogenesis and immunity. Trends Microbiol 2003;11: 87-93.
22. Van der Ley P, Heckels JE, Virji M, Hoogerhout P, Poolman JT. Topology of outer membrane porins in pathogenic Neisseria spp. Infect Immun 1991;59:2963-2971.
23. Thompson DK, Deal CD, Ison CA, Zenilman JM, Bash MC. A typing system for Neisseria gonorrhoeae based on biotinylated oligonucleotide probes to PIB gene variable regions. J Infect Dis 2000;181:1652-1660.
24. McKnew DL, Lynn F, Zenilman JM, Bash MC. Porin variation among clinical isolates of Neisseria gonorrhoeae over a 10-year period, as determined by Por variable region typing. J Infect Dis 2003;187:1213-1222.
25. Giles JA, Falconio J, Yuenger JD, Zenilman J, Dan M, Bash MC. Quinolone resistance-determining region mutations and por type of Neisseria gonorrhoeae isolates: resistance surveillance and typing by molecular methodologies. J Infect Dis 2004;189: 2085-2093.
26. Bash MC, Zhu P, Gulati S, McKnew D, Rice PA, Lynn F. por variable-region typing by DNA probe hybridization is broadly applicable to epidemiologic studies of Neisseria gonorrhoeae. J Clin Microbiol 2005;43:1522-1530.
27. Lynn F, Hobbs MM, Zenilman JM, et al. Genetic typing of the porin protein of Neisseria gonorrhoeae from clinical noncultured samples: Strain characterisation and identification of mixed gonococcal infections. J Clin Microbiol 2005;43:368-375.
28. Tabrizi SN, Fairley CK, Chen S, et al. Evaluation of patient-administered tampon specimens for Chlamydia trachomatis and Neisseria gonorrhoeae. Sex Transm Dis 2000;27:133-137.
29. Tabrizi SN, Chen S, Tapsall J, Garland SM. Evaluation of opa-based real-time PCR for detection of Neisseria gonorrhoeae. Sex Transm Dis 2005;32:199-202.
30. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403-410.
31. Johnson RE, Newhall WJ, Papp JR, et al. Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections. MMWR Recomm Rep 2002;51:1-38.
32. Whiley DM, Buda PP, Freeman K, Pattle NI, Bates J, Sloots TP. A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens. Diagn Microbiol Infect Dis 2005;52:1-5.
33. Knapp JS, Hook EW. Prevalence and persistence of Neisseria cinerea and other Neisseria spp. in adults. J Clin Microbiol 1988; 26:896-900.