A real-time PCR assay for the detection of Neisseria gonorrhoeae in genital and extragenital specimens

Diagnostic Microbiology and Infectious Disease

Volumn 52, Issue 1, 2005, Pages 1-5

  Whiley, David M ^a,b   Buda, Philip P ^c   Freeman, Kevin ^d   Pattle, Neville I ^e   Bates, John ^f   Sloots, Theo P ^a,b,c,g  

^a ROYAL CHILDREN'S HOSPITAL   (Australia)

^b UNIVERSITY OF QUEENSLAND   (Australia)

^c QUEENSLAND HEALTH   (Australia)

^d ROYAL DARWIN HOSPITAL   (Australia)

^e Gippsland Pathology Service   (Australia)

^f QLD Health Scientific Services   (Australia)

^g ROYAL CHILDREN'S HOSPITAL   (Australia)

Author keywords

LightCycler; Neisseria gonorrhoeae; Real time PCR

Indexed keywords

ARTICLE; BACTERIAL GENE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FEMALE; GENE TARGETING; GENITAL TRACT INFECTION; HUMAN; INTERMETHOD COMPARISON; NEISSERIA GONORRHOEAE; PRIORITY JOURNAL; PSEUDOGENE; REAL TIME POLYMERASE CHAIN REACTION; RECTUM; SENSITIVITY AND SPECIFICITY; THROAT CULTURE; URETHRA; UTERINE CERVIX;

CULTURE MEDIA; DNA, BACTERIAL; FEMALE; GENITALIA, FEMALE; GENITALIA, MALE; GONORRHEA; HUMANS; MALE; NEISSERIA GONORRHOEAE; POLYMERASE CHAIN REACTION; PORINS; PSEUDOGENES; SENSITIVITY AND SPECIFICITY;

EID: 18844394325     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2004.12.011     Document Type: Article

References (16)

1. D.J. Diemert, M.D. Libman, and P. Lebel Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG test for diagnosis of Neisseria gonorrhoeae infection in a low-prevalence population J. Clin. Microbiol. 40 2002 4056 4059
2. D.J. Farrell Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR J. Clin. Microbiol. 37 1999 386 390
3. C.P. Gibbs, and T.F. Meyer Genome plasticity in Neisseria gonorrhoeae FEMS Microbiol. Lett. 145 1996 173 179
4. B. Hughes, M.T. Pezzlo, L.M. de la Maza, and E.M. Peterson Rapid identification of pathogenic Neisseria species and Branhamella catarrhalis J. Clin. Microbiol. 25 1987 2223 2224
5. R.E. Johnson, W.J. Newhall, J.R. Papp, J.S. Knapp, C.M. Black, T.L. Gift, R. Steece, L.E. Markowitz, O.J. Devine, C.M. Walsh, S. Wang, D.C. Gunter, K.L. Irwin, S. DeLisle, and S.M. Berman Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections-2002 MMWR Recomm. Rep. 18 2002 1 38
6. K.A. Kline, E.V. Sechman, E.P. Skaar, and H.S. Seifert Recombination, repair and replication in the pathogenic neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens Mol. Microbiol. 50 2003 3 13
7. D.E. Leslie, F. Azzato, N. Ryan, and J. Fyfe An assessment of the Roche Amplicor Chlamydia trachomatis/Neisseria gonorrhoeae multiplex PCR assay in routine diagnostic use on a variety of specimen types Commun. Dis. Intell. 27 2003 373 379
8. B. Linz, M. Schenker, P. Zhu, and M. Achtman Frequent interspecific genetic exchange between commensal neisseriae and Neisseria meningitidis Mol. Microbiol. 36 2000 1049 1058
9. K. Page-Shafer, A. Graves, C. Kent, J.E. Balls, V.M. Zapitz, and J.D. Klausner Increased sensitivity of DNA amplification testing for the detection of pharyngeal gonorrhea in men who have sex with men Clin. Infect. Dis. 15 2002 173 176
10. H.M. Palmer, H. Mallinson, R.L. Wood, and A.J. Herring Evaluation of the specificities of five DNA amplification methods for the detection of Neisseria gonorrhoeae J. Clin. Microbiol. 41 2003 835 837
11. D.M. Whiley, G.M. LeCornec, I.M. Mackay, D.J. Siebert, and T.P. Sloots A real-time PCR assay for the detection of Neisseria gonorrhoeae by LightCycler Diagn. Microbiol. Infect. Dis. 42 2002 85 89
12. D.M. Whiley, I.M. Mackay, M.W. Syrmis, M.J. Witt, and T.P. Sloots Detection and differentiation of herpes simplex virus types 1 and 2 by a duplex LightCycler PCR that incorporates an internal control PCR reaction J. Clin. Virol. 30 2004 32 38
13. D.M. Whiley, P.J. Buda, J. Bayliss, L. Cover, J. Bates, and T.P. Sloots A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene Eur. J. Clin. Microbiol. Infect. Dis. 23 2004 705 710
14. I.G. Wilson Inhibition and facilitation of nucleic acid amplification Appl. Environ. Microbiol. 63 1997 3741 3751
15. H. Young, K. Manavi, and A. McMillan Evaluation of ligase chain reaction for the non-cultural detection of rectal and pharyngeal gonorrhoea in men who have sex with men Sex. Transm. Infect. 79 2003 484 486
16. C.C. Yuan, W. Miley, and D. Waters A quantification of human cells using an ERV-3 real time PCR assay J. Virol. Methods 91 2001 109 117