Non-invasive and simple methods for sampling rabbit DNA for PCR analysis of melanocortin 1 receptor (MC1R) gene mutations: A technical note

World Rabbit Science

Volumn 15, Issue 2, 2007, Pages 121-126

  Fontanesi, L ^a   Tazzoli, M ^a   Russo, V ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Biological specimens; Buccal swabs; Direct PCR; Hair DNA; MC1R mutations; Rabbit

Indexed keywords

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 34547395941     PISSN: 12575011     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Brooks R., Williamson J., Hensley A., Butler E., Touchton G., Smith E., 2003. Buccal cells as a source of DNA for comparative animal genomic analysis. Biotechnology Lett., 25, 451-454.
2. Fode-Vaughan K.A., Wimpee C.F., Remsen C.C., Collins M.L., 2001. Detection of bacteria in environmental samples by direct PCR without DNA extraction. Biotechniques, 31, 598-604.
3. Fontanesi L., Tazzoli M., Beretti F., Russo V., 2006. Mutations in the melanocortin 1 receptor (MC1R) gene are associated with coat colours in the domestic rabbit (Oryctolagus cuniculus). Anim. Genet., 37, 489-493.
4. Frantz A.C., Schaul M., Pope L.C., Fack F., Schley L., Muller C.P., Roper T.J., 2004. Estimating population size by genotyping remotely plucked hair: the Eurasian badger. J. Appl. Ecol., 41, 985-995.
5. Feigelson H.S., Rodriguez C., Robertson A.S., Jacobs E.J., Calle E.E., Reid Y.A., Thun M.J., 2001. Determinants of DNA yield and quality from buccal cell samples collected with mouthwash. Cancer Epidemiol. Biomarkers Prev., 10, 1005-1008.
6. French D.J., Archard C.L., Andersen M.T., McDowell D.G., 2002. Ultra-rapid DNA analysis using HyBeacon probes and direct PCR amplification from saliva. Mol. Cell. Probes, 16, 319-326.
7. Goossens B., Waits L.P., Taberlet P., 1998. Plucked hair samples as a sources of DNA: reliability of dinucleotide microsatellite genotyping. Mol. Ecol., 7, 1237-1241.
8. Healy P.J., Dennis J.A., Moule J.F., 1995. Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle. Aust. Vet. J., 72, 392.
9. Higuchi R., Von Beroldingen C.H., Sensabaugh G.F., Erlich H.A., 1988. DNA typing from single hairs. Nature, 332, 543-546.
10. Liu Y.S., Thomas R.J.S., Phillips W.A., 1995. Single-step direct PCR amplification from solid tissues. Nucleic Acids Res., 23, 1640.
11. Meldgaard M., Bollen P.J.A., Finsen B., 2004. Non-invasive method for sampling and extraction of mouse DNA for PCR. Lab. Anim., 38, 413-417.
12. Melton T., Dimick G., Higgins B., Lindstrom L., Nelson K., 2005. Forensic mitochondrial DNA analysis of 691 casework hairs. J. Forensic Sci., 50, 73-80.
13. Menotti-Raymond M.A., David V.A., O'Brien S.J., 1997. Pet cat hair implicates murder suspect. Nature, 386, 774.
14. Mercier B., Gaucher C., Feugeas O., Mazurier C., 1990. Direct PCR from whole blood, without DNA extraction. Nucleic Acids Res., 18, 5908.
15. Oberbauer A.M., Grossman D.I., Eggleston M.L., Irion D.N., Schaffer A.L., Pedersen N.C., Belanger J.M., 2003. Alternatives to blood as a source of DNA for large-scale scanning studies of canine genome linkages. Vet. Res. Commun., 27, 27-38.
16. Panaccio M., Georgesz M., Hollywell C., Lew A., 1993a. Direct PCR from solid tissues without DNA extraction. Nucleic Acids Res., 21, 4656.
17. Panaccio M., Georgesz M., Lew A.M., 1993b. FoLT PCR: a simple PCR protocol for amplifying DNA directly from whole blood. Biotechniques, 14, 238-243.
18. Pfeiffer I., Völkel I., Täubert H., Brenig B., 2004. Forensic DNA-typing of dog hair: DNA-extraction and PCR amplification. Forensic Sci. Int., 141, 149-151.
19. Roon D.A., Waits L.P., Kendall K.C., 2003. A quantitative evaluation of two methods for preserving hair samples. Mol. Ecol. Notes, 3, 163-166.
20. Russo V., Dall'Olio S., Davoli R., Costelli M.B., Bigi D., 1996. Studio del locus Alotano nelle razze suine allevate in Italia. Zootec. Nutr. Anim., 22, 33-38.
21. Sambrook J., Fritsch E.F., Maniatis T., 1989. Molecular cloning: a laboratory manual. Second edition. Cold Spring Harbor Laboratory Press, NY, USA.
22. Sandford A.J., Pare P.D., 1997. Direct PCR of small genomic DNA fragments from serum. Biotechniques, 23, 890-892.
23. Sloane M.A., Sunnucks P., Alpers D., Beheregaray L.B., Taylor A.C., 2000. Highly reliable genetic identification of individual northern hairy-nosed wombats from single remotely collected hairs: a feasible censuring method. Mol. Ecol., 9, 1233-1240.
24. Thomson D.M., Brown N.N., Clague A.E., 1992. Routine use of hair root or buccal swab specimens for PCR analysis: Advantages over using blood. Clin. Chim. Acta, 207, 169-174.
25. Walker A.H., Najarian D., White D.L., Jaffe J.M., Kanetsky P.A., Rebbeck T.R., 1999. Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays. Environm. Health Perspectives, 107, 517-520.
26. Wong L.-J.C., Lueth M., Li X.-N., Lau C.C., Vogel H., 2003. Detection of mitochondrial DNA mutations in the tumor and cerebrospinal fluid of medulloblastoma patients. Cancer Res., 63, 3866-3871.