Direct PCR of small genomic DNA fragments from serum

BioTechniques

Volumn 23, Issue 5, 1997, Pages 890-892

  Sandford, Andrew J ^a   Paré, Peter D ^a,b  

^a The James Hogg iCAPTURE Centre for Cardiovascular and Pulmonary Research   (Canada)

^b ST PAUL S HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DIAGNOSTIC VALUE; DNA DETERMINATION; GENE SEQUENCE; GENOTYPE; HUMAN; POLYMERASE CHAIN REACTION;

EID: 0030707946     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97235st05     Document Type: Article

References (6)

1. Cheyrou, A., C. Guyomarc'h, P. Jasserand and P. Blouin. 1991. Improved detection of HBV DNA by PCR after microwave treatment of serum. Nucleic Acids Res. 19:4006.
2. Costa, J., F.X. Lopez-Labrador, J.M. Sanchez-Tapias, A. Mas, A. Vilella, E. Olmedo, J. Vidal, M.T. Jimenez de Anta and J. Rodes. 1995. Microwave treatment of serum facilitates detection of hepatitis B virus DNA by the polymerase chain reaction. J. Hepatol. 22:35-42.
3. Fowke, K.R., F.A. Plummer and J.N. Simonsen. 1995. Genetic analysis of human DNA recovered from minute amounts of serum or plasma. J. Immunol. Methods 180:45-51.
4. Martin, M., M. Carrington and D. Mann. 1992. A method for using serum or plasma as a source of DNA for HLA typing. Hum. Immunol. 33:108-113.
5. Ohhara, M., Y. Kurosu and M. Esumi. 1994. Direct PCR of whole blood and hair shafts by microwave treatments. BioTechniques 17:726-728.
6. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring, Harbor, NY.