Air-exposure technique for the formation of artificial lipid bilayers in microsystems

Langmuir

Volumn 23, Issue 15, 2007, Pages 8277-8284

  Sandison, Mairi E ^a   Zagnoni, Michele ^a   Morgan, Hywel ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; LIPID BILAYERS; MICROFLUIDICS; PROTEINS;

AIR-EXPOSURE PERIOD; ANALYTES; BILAYER LIPID MEMBRANE (BLM); LIPID-SOLVENT LAYER; THINNING;

MICROSYSTEMS;

AIR; ARTICLE; GENETIC PROCEDURES; LIPID BILAYER; MICROFLUIDIC ANALYSIS; PHASE TRANSITION;

AIR; BIOSENSING TECHNIQUES; LIPID BILAYERS; MICROFLUIDIC ANALYTICAL TECHNIQUES; PHASE TRANSITION;

EID: 34547312344     PISSN: 07437463     EISSN: None     Source Type: Journal    
DOI: 10.1021/la7007528     Document Type: Article

References (33)

1. Mueller, P.; Rudin, D. O.; Tien, H. T.; Wescott, W. C. Reconstitution of cell membrane structure in vitro and its transformation into an excitable system. Nature 1962, 194, 979-80.
2. White S. H. The physical nature of planar bilayer membranes. In Ion Channel Reconstitution; Miller, C., Ed; Plenum Press: New York, 1986; pp 3-35.
3. Osborn, T. D.; Yager, P. Formation of planar solvent-free phospholipid bilayers by Langmuir-Blodgett transfer of monolayers to micromachined apertures in silicon. Langmuir 1995, 11, 8-12.
4. Schmidt, C.; Mayer, M.; Vogel, H. A chip-based biosensor for the functional analysis of single ion channels. Angew. Chem., Int. Ed. 2000, 39, 3137-3140.
5. Eray, M.; Dogan, N. S.; Liu, L. J.; Koch, A. R.; Moffett, D. F.; Silber, M.; Vanwie, B. J. Highly stable bilayer lipid membranes (BLMs) formed on microfabricated polyimide apertures. Biosens. Bioelectron. 1994, 9, 343-351.
6. Peterman, M. C.; Ziebarth, J. M.; Braha, O.; Bayley, H.; Fishman, H. A.; Bloom D. M. Ion channels and lipid bilayer membranes under high potentials using microfabricated apertures. Biomed. Microdevices 2002, 4, 231-236.
7. Pantoja, R.; Sigg, D.; Blunck, R.; Bezanilla, F.; Heath, J. R. Bilayer reconstitution of voltage-dependent ion channels using a microfabricated silicon chip. Biophys. J. 2001, 81, 2389-2394.
8. Wilk, S. J.; Goryll, M.; Laws, G. M.; Goodnick, S. M.; Thornton, T. J.; Saraniti, M.; Tang, J.; Eisenberg, R. S. Teflon-coated silicon apertures for supported lipid bilayer membranes. Appl. Phys. Lett. 2004, 85, 3307-3309.
9. Morgan, H.; Sandison, M. E.; Mendes, G.; Berry, R.; Watts, A. Artificial Lipid Bilayers in a Microfabricated System, In Proceedings of μTAS 2004, The 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 2004; Vol. 1, pp 330-332.
10. Mayer, M.; Kriebel, J. K.; Tosteson, M. T.; Whitesides, G. M. Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayers. Biophys. J. 2003, 85, 2684-2695.
11. Cheng, Y.; Bushby, R. J.; Evans, S. D.; Knowles, P. F.; Miles, R. E.; Ogier, S.D. Single ion channel sensitivity in suspended bilayers on micromachined supports. Langmuir 2001, 17, 1240-1242.
12. Fertig, N.; Meyer, C.; Blick, R. H.; Trautmann, C.; Behrends, J. C. Microstructured glass chip for ion channel electrophysiology. Phys. Rev. E 2001, 64, 40901/1-40901/4.
13. Suzuki, H.; Tabata, K.; Kato-Yamada, Y.; Noji, H.; Takeuchi, S. Planar Lipid Bilayer Chip for Electrophysiological Analysis of Membrane Proteins, In Proceedings of μTAS 2004, The 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 2004; Vol. 2, pp 246-248.
14. Sandison, M. E.; Morgan, H. Rapid fabrication of polymer microfluidic systems for the production of artificial lipid bilayers. J. Micromech. Microeng. 2005, 15, 139-144.
15. Suzuki, H.; Tabata, K. V.; Noji, H.; Takeuchi, S. Highly reproducible method of planar lipid bilayer reconstitution in a polymethyl methacrylate microfluidic chip. Langmuir 2006, 22, 1937-1942.
16. Suzuki, H.; Tabata, K. V.; Noji, H.; Takeuchi, S. Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip. Biosens. Bioelectron. 2007, 22, 1111-1115.
17. Ide, T.; Ichikawa, T. A novel method for artificial lipid bilayer formation. Biosens. Bioelectron. 2005, 21, 672-677.
18. Tien, H. T.; Dawidowicz, E. A. Black lipid films in aqueous media: A new type of interfacial phenomenon. Experimental techniques and thickness measurements. J. Colloid Interface Sci. 1966, 22, 438-453.
19. Fujiwara, H.; Fujihara, M.; Ishiwata, T. Dynamics of the spontaneous formation of a planar phospholipid bilayer: A new approach by simultaneous electrical and optical measurements. J. Chem. Phys. 2003, 119, 6768-6775.
20. Kalinowski, S.; lotowski, Z.; Morzycki, J. W. The influence of a bolaamphiphilic steroid dimer on the formation and structure of bilayer lipid membranes. Cell. Biol. Mol. Lett. 2000, 5, 107-118.
21. Suzuki, H.; Tabata, K.; Kato-Yamada, Y.; Noji, H.; Takeuchi, S. Planar lipid bilayer reconstitution with a microfluidic system. Lab Chip 2004, 4, 502-505.
22. If a precise volume of lipid (corresponding to that required to form a ∼100 nm thick layer) could be injected directly, a trench would be unnecessary. However, this volume would be on the order of nanoliters, which is not feasible using standard, inexpensive microsyringes.
23. Wang, J.; Pumera, M.; Chatrathi, M. P.; Escarpa, A.; Konrad, R.; Griebel, A.; Dörner, W.; Löwe, H. Towards disposable lab-on-a-chip: Poly(methylmethacrylate) microchip electrophoresis device with electrochemical detection. Electrophoresis 2002, 23, 596-601.
24. de Planque, M. R. R.; Mendes, G. P.; Zagnoni, M.; Sandison, M. E.; Fisher, K. H.; Berry, R. M.; Watts, A.; Morgan, H. Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion. IEEE Proc. Nanobiotechnol. 2006, 153, 21-30.
25. Hydrophobic, fluorinated polymers such as Teflon could be used for producing microfluidic devices, but the fabrication process would be more complicated, due to the higher glass transition temperatures of these polymers and the difficulty in bonding multilayer structures from them.
26. Shah, J. J.; Geist, J.; Locascio, L. E.; Gaitan, V.; Rao, M. V.; Vreeland, W.N. Surface modification of poly(methylmethacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis. Electrophoresis 2006, 27, 3788-3796.
27. It was sometimes observed that the bilayer area changed slightly during the first few seconds after buffer injection. However, once the lipid structure had formed a stable membrane, no further changes were observed during the lifetime of the BLM (except for an occasional slight shrinkage or growth in the BLM area over the course of several hours).
28. Andersen, O. S.; Koeppe, R. E., II; Roux, B. Gramicidin channels. IEEE Trans. Nanobiosci. 2005, 4, 10-20.
29. Sandison, M. E.; Zagnoni, M.; Abu-Hantash, M.; Morgan, H. Micromachined glass apertures for artificial bilayer lipid membrane formation in a microfluidic system. J. Micromech. Microeng., accepted.
30. Jeon, T.-J.; Malmstadt, N.; Schmidt, J. J. Hydrogel-encapsulated lipid membranes. J. Am. Chem. Soc. 2006, 128, 42-43.
31. Costello, R. F.; Peterson, I. R.; Heptinstall, J.; Walton, D. J. Improved gel-protected bilayers. Biosens. Bioelectron. 1999, 14, 265-271.
32. Zagnoni, M.; Sandison, M. E.; Wood, R. J.; Roach, P. L.; Morgan, H. Controlled Delivery of Calcium-Gated Mthk Channels into an On-Chip Bilayer Lipid Membrane for Electrophysiology Measurements, In Proceedings of μTAS 2006, The 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences: 2006; Vol. 2, pp 1342-1344.
33. Zagnoni, M.; Sandison, M. E.; Marius, P.; Lee, A. G.; Morgan, H. Controlled delivery of proteins into bilayer lipid membranes on chip. Lab Chip, In press. DOI: 10.1039/B703818F.