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2 were monitored by fluorescence spectrophotometry. SC cleaves the peptide bond in N-succinyl-L-ala-L-ala- L-pro-L-phe-p-nitroanilide to release a chromophore, p-nitroaniline. and the ini tial rates were obtained by measuring the increase in the absorbance at 405 nm.
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2 were monitored by fluorescence spectrophotometry. SC cleaves the peptide bond in N-succinyl-L-ala-L-ala- L-pro-L-phe-p-nitroanilide to release a chromophore, p-nitroaniline. and the ini tial rates were obtained by measuring the increase in the absorbance at 405 nm.
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The silica nanoparticles and silica microspheres were purchased from EKA Chemicals, Inc, Augusta, GA) and Bangs Laboratories, Inc, Fishers. IN, respectively, and the particles were rendered hydrophobic by treating them with octadecyltrimethoxysilane OTMS, The aggregate size of the hydrophobic silica nanoparticles was determined by scanning electron microscopy
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The silica nanoparticles and silica microspheres were purchased from EKA Chemicals, Inc. (Augusta, GA) and Bangs Laboratories, Inc. (Fishers. IN), respectively, and the particles were rendered hydrophobic by treating them with octadecyltrimethoxysilane (OTMS). The aggregate size of the hydrophobic silica nanoparticles was determined by scanning electron microscopy.
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46749095692
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Gold nanoparticles were prepared by reducing tetrachloroauric acid with trisodium citrate. The surface of gold nanoparticles and the flat gold chips were rendered hydrophobic by exposing them to a solution of undecanethiol. The aggregate size of the hydrophobic gold nanoparticles was determined by scanning electron microscopy
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Gold nanoparticles were prepared by reducing tetrachloroauric acid with trisodium citrate. The surface of gold nanoparticles and the flat gold chips were rendered hydrophobic by exposing them to a solution of undecanethiol. The aggregate size of the hydrophobic gold nanoparticles was determined by scanning electron microscopy.
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The pA-nanoparticle-enzyme films were prepared by dispersing the nanoparticle-enzyme conjugates in a solution of acrylamide and then allowing the mixture to polymerize by adding ammonium persulfate
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The pA-nanoparticle-enzyme films were prepared by dispersing the nanoparticle-enzyme conjugates in a solution of acrylamide and then allowing the mixture to polymerize by adding ammonium persulfate.
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