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Volumn 7, Issue 4-5, 2007, Pages 1675-1678

Enhanced stability of enzymes adsorbed onto nanoparticles

Author keywords

Biocatalytic films; Enzyme stability; Fullerenes; Nanoparticles

Indexed keywords

CURVED SURFACES; ENHANCED STABILITY;

EID: 34447519151     PISSN: 15334880     EISSN: None     Source Type: Journal    
DOI: 10.1166/jnn.2007.453     Document Type: Article
Times cited : (62)

References (36)
  • 24
    • 84857896232 scopus 로고    scopus 로고
    • 2 were monitored by fluorescence spectrophotometry. SC cleaves the peptide bond in N-succinyl-L-ala-L-ala- L-pro-L-phe-p-nitroanilide to release a chromophore, p-nitroaniline. and the ini tial rates were obtained by measuring the increase in the absorbance at 405 nm.
    • 2 were monitored by fluorescence spectrophotometry. SC cleaves the peptide bond in N-succinyl-L-ala-L-ala- L-pro-L-phe-p-nitroanilide to release a chromophore, p-nitroaniline. and the ini tial rates were obtained by measuring the increase in the absorbance at 405 nm.
  • 30
    • 46749089337 scopus 로고    scopus 로고
    • The silica nanoparticles and silica microspheres were purchased from EKA Chemicals, Inc, Augusta, GA) and Bangs Laboratories, Inc, Fishers. IN, respectively, and the particles were rendered hydrophobic by treating them with octadecyltrimethoxysilane OTMS, The aggregate size of the hydrophobic silica nanoparticles was determined by scanning electron microscopy
    • The silica nanoparticles and silica microspheres were purchased from EKA Chemicals, Inc. (Augusta, GA) and Bangs Laboratories, Inc. (Fishers. IN), respectively, and the particles were rendered hydrophobic by treating them with octadecyltrimethoxysilane (OTMS). The aggregate size of the hydrophobic silica nanoparticles was determined by scanning electron microscopy.
  • 31
    • 46749095692 scopus 로고    scopus 로고
    • Gold nanoparticles were prepared by reducing tetrachloroauric acid with trisodium citrate. The surface of gold nanoparticles and the flat gold chips were rendered hydrophobic by exposing them to a solution of undecanethiol. The aggregate size of the hydrophobic gold nanoparticles was determined by scanning electron microscopy
    • Gold nanoparticles were prepared by reducing tetrachloroauric acid with trisodium citrate. The surface of gold nanoparticles and the flat gold chips were rendered hydrophobic by exposing them to a solution of undecanethiol. The aggregate size of the hydrophobic gold nanoparticles was determined by scanning electron microscopy.
  • 36
    • 46749105673 scopus 로고    scopus 로고
    • The pA-nanoparticle-enzyme films were prepared by dispersing the nanoparticle-enzyme conjugates in a solution of acrylamide and then allowing the mixture to polymerize by adding ammonium persulfate
    • The pA-nanoparticle-enzyme films were prepared by dispersing the nanoparticle-enzyme conjugates in a solution of acrylamide and then allowing the mixture to polymerize by adding ammonium persulfate.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.