Comparison of three chromogenic media and evaluation of two molecular-based identification systems for the detection of Enterobacter sakazakii irom environmental samples from infant formulae factories

Journal of Food Protection

Volumn 70, Issue 7, 2007, Pages 1678-1684

  Derzelle, Sylviane ^a   Dilasser, Françoise ^a   Maladen, Véronique ^a   Soudrie, Nicole ^a   Leclercq, Alexandre ^b   Lombard, Bertrand ^a   Lafarge, Véronique ^a  

^a Healthy Pigs Production and Experimentation Section   (France)

^b INSTITUT PASTEUR   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ENTEROBACTER SAKAZAKII; ENTEROBACTERIACEAE;

EID: 34447513121     PISSN: 0362028X     EISSN: None     Source Type: Journal    
DOI: 10.4315/0362-028X-70.7.1678     Document Type: Article

References (36)

1. Anonymous. 2005. Milk and milk products - detection of Enterobacter sakazakii. 22964. IDF RM 210. International Organization for Standardization, Geneva.
2. Arseni, A., E. Malamou-Ladas, C. Koutsia, M. Xanthou, and E. Trikka. 1987. Outbreak of colonization of neonates with Enterobacter sakazakii. J. Hosp. Infect. 9:143-150.
3. Bar-Oz, B., A. Preminger, O. Peleg, C. Block, and I. Arad. 2001. Enterobacter sakazakii infection in the newborn. Acta Paediatr. 90:356-358.
4. Biering, G., S. Karlsson, N. C. Clark, K. Jonsdottir, P. Ludvigsson, and O. Steingrimsson. 1989. Three cases of neonatal meningitis caused by Enterobacter sakazakii in powdered milk. J. Clin. Microbiol. 27:2054-2056.
5. Block, C., O. Peleg, N. Minster, B. Bar-Oz, A. Simonh, I. Arad, and M. Shapiro. 2002. Cluster of neonatal infections in Jerusalem due to unusual biochemical variant of Enterobacter sakazakii. Eur. J. Clin. Microbiol. Infect. Dis. 21:613-616.
6. Bowen, A. B., and C. R. Braden. 2006. Invasive Enterobacter sakazakii disease in infants. Emerg. Infect. Dis. 12:1185-1189.
7. Derzelle, S., and F. Dilasser. 2006. A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae. BMC Microbiol. 6:100.
8. Drudy, D., M. O'Rourke, M. Murphy, N. R. Mullane, R. O'Mahony, L. Kelly, M. Fischer, S. Sanjaq, P. Shannon, P. Wall, M. O'Mahony, P. Whyte, and S. Fanning. 2006. Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources. Int. J. Food Microbiol. 110:127-134.
9. Farmer, J. J., M. A. Asbury, F. W. Hickman, and D. J. Brenner. 1980. Enterobacter sakazakii: a new species of "Enterobacteriaceae" isolated from clinical specimens. Int. J. Syst. Bacteriol. 30:569-584.
10. Food and Agriculture Organization and World Health Organization. 2006. Enterobacter sakazakii and Salmonella in powdered infant formula: meeting report. Microbiological risk assessment. Series 10. Available at: http://www.fao.org/ag/AGN/jemra/enterobacter_en.stm. Accessed 23 November 2006.
11. Gnanou Besse, N., A. Leclercq, V. Maladen, C. Tyburski, and B. Lombard. 2006. Evaluation of the ISO-IDF draft standard method for the detection of Enterobacter sakazakii in milk powder products and powder infant formula. J. AOAC Int. 89:1309-1316.
12. Guillaume-Gentil, O., V. Sonnard, M. C. Kandhai, J. D. Marugg, and H. Joosten. 2005. A simple and rapid cultural method for detection of Enterobacter sakazakii in environmental samples. J. Food Prot. 68:64-69.
13. Gurtler, J. B., J. L. Kornacki, and L. R. Beuchat. 2005. Enterobacter sakazakii: a coliform of increased concern to infant health. Int. J. Food Microbiol. 104:1-34.
14. Iversen, C., P. Druggan, and S. Forsythe. 2004. A selective differential medium for Enterobacter sakazakii, a preliminary study. Int. J. Food Microbiol. 96:133-139.
15. Iversen, C., and S. J. Forsythe. 2003. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci. Technol. 14:443-454.
16. Iversen, C., and S. J. Forsythe. 2004. Isolation of Enterobacter sakazakii and other Enterobacteriaceae from powdered infant formula milk and related products. Food Microbiol. 21:771-777.
17. Iversen, C., and S. J. Forsythe. 2007. Comparison of media for the isolation of Enterobacter sakazakii. Appl. Environ. Microbiol. 73:48-52.
18. Iversen, C., M. Lane, and S. Forsythe. 2004. The growth profile, thermotolerance and biofilm formation of Enterobacter sakazakii grown in infant formula milk. Lett. Appl. Microbiol. 38:378-382.
19. Iversen, C., M. Waddington, S. L. W. On, and S. Forsythe. 2004. Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J. Clin. Microbiol. 42:5368-5370.
20. Jung, M.-K., and J.-H. Park. 2006. Prevalence and thermal stability of Enterobacter sakazakii form unprocessed ready-to-eat agricultural products and powdered infant formulas. Food Sci. Biotechnol. 15:152-157.
21. Kandhai, M. C., M. W. Reij, L. G. Gorris, O. Guillaume-Gentil, and M. van Schofhorst. 2004. Occurrence of Enterobacter sakazakii in food production environments and households. Lancet 363:39-40.
22. Kandhai, M. C., M. W. Reij, K. van Puyvelde, O. Guillaume-Gentil, R. R. Beumer, and M. Van Schothorst. 2004. A new protocol for the detection of Enterobacter sakazakii applied to environmental samples. J. Food Prot. 67:1267-1270.
23. Lai, K. K. 2001. Enterobacter sakazakii infections among neonates, infants, children, and adults. Case reports and a review of the literature. Medicine (Baltimore) 80:113-122.
24. Lehner, A., S. Nitzsche, P. Breeuwer, B. Diep, K. Thelen, and R. Stephan. 2006. Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection. BMC Microbiol. 6:15.
25. Lehner, A., T. Tasara, and R. Stephan. 2004. 16S rRNA gene based analysis of Enterobacter sakazakii strains from different sources and development of a PCR assay for identification. BMC Microbiol. 4:43.
26. Liu, Y., X. Cai, X. Zhang, Q. Gao, X. Yang, Z. Zheng, M. Luo, and X. Huang. 2005. Real time PCR using TaqMan and SYBR Green for detection of Enterobacter sakazakii in infant formula. J. Microbiol. Methods 65:21-31.
27. Malorny, B., and M. Wagner. 2005. Detection of Enterobacter sakazakii strains by real-time PCR. J. Food Prot. 68:1623-1627.
28. Muytjens, H. L., H. Roelofs-Willemse, and G. H. Jaspar. 1988. Quality of powdered substitutes for breast milk with regard to members of the family Enterobacteriaceae. J. Clin. Microbiol. 26:743-746.
29. Nair, M., and K. S. Venkitanarayanan. 2006. Cloning and sequencing of the ompA gene of Enterobacter sakazakii and development of an ompA-targeted PCR for rapid detection of Enterobacter sakazakii in infant formula. Appl. Environ. Microbiol. 72:2539-2546.
30. Nazarowec-White, M., and J. M. Farber. 1997. Incidence, survival, and growth of Enterobacter sakazakii in reconstituted dried-infant formula. Lett. Appl. Microbiol. 24:9-13.
31. Restaino, L., E. W. Frampton, W. C. Lionberg, and R. J. Becker. 2006. A chromogenic plating medium for the isolation and identification of Enterobacter sakazakii from foods, food ingredients, and environmental sources. J. Food Prot. 69:315-322.
32. Seo, K. H., and R. E. Brackett. 2005. Rapid, specific detection of Enterobacter sakazakii in infant formula using a real-time PCR assay. J. Food Prot. 68:59-63.
33. Van Acker, J., F. de Smet, G. Muyldermans, A. Bougatef, A. Naessens, and S. Lauwers. 2001. Outbreak of necrotizing enterocolitis associated with Enterobacter sakazakii in powdered milk formula. J. Clin. Microbiol. 39:293-297.
34. Weir, E. 2002. Powdered infant formula and fatal infection with Enterobacter sakazakii. Can. Med. Assoc. J. 166:1570.
35. Weiss, C., B. Becker, and W. Holzapel. 2005. Einsatz und Eignung dreier kommerzieller Systeme zum Nachweis van Enterobacter sakazakii in verzehrsfertigem Mischsalat. Archiv. Lebensmittelhyg. 56:34-38.
36. Zink, D. 2003. FDA field survey of powdered formula manufacturing. Presented at a U.S. Food and Drug Administration Food Advisory Committee meeting.