Derivation and characterization of monoclonal antibodies against human folypolyglutamate synthetase

Hybridoma

Volumn 26, Issue 3, 2007, Pages 155-161

  Dotzlaf, Joe ^a   Carpenter, John ^a   Luo, Shuang ^a   Miles, Rebecca R ^a   Fisher, Deborah ^a   Qian, Yue Wei ^a   Ehsani, Mariam ^a   Wang, Xiliang ^a   Lin, Aimin ^a   Mcclure, Don B ^a   Chen, Victor J ^a   Zuckerman, Steven H ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FOLIC ACID ANTAGONIST; FOLIC ACID DERIVATIVE; FOLYPOLYGLUTAMATE SYNTHETASE; GLUTAMATE SYNTHASE; IMMUNOGLOBULIN G1 ANTIBODY; MESSENGER RNA; MONOCLONAL ANTIBODY; POLYCLONAL ANTISERUM; UNCLASSIFIED DRUG;

ARTICLE; CELL LYSATE; DERIVATIZATION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME SPECIFICITY; EPITOPE MAPPING; HELA CELL; HUMAN; HUMAN CELL; KINETICS; MOLECULAR RECOGNITION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RNA INTERFERENCE; TUMOR BIOPSY; TUMOR CELL; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; EPITOPE MAPPING; HELA CELLS; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN G; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; NEOPLASMS; PEPTIDE FRAGMENTS; PEPTIDE SYNTHASES; PROTEIN CONFORMATION; PROTEIN DENATURATION; RNA INTERFERENCE; RNA, SMALL INTERFERING; SURFACE PLASMON RESONANCE;

EID: 34447323855     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2007.004     Document Type: Article

References (15)

1. Moran RG: Roles of folypoly-γ-glutamate synthetase in therapeutics with tetrahydrofolate antimetabolites: an overview. Semin Oncol 1999;26:24-32.
2. Goldman ID and Zhao R: Molecular, biochemical, and cellular pharmacology of Pemetrexed. Semin Oncol 2002;29:3-17.
3. Gangjee A, Dubash NP, Zeng Y, and McGuire JJ: Recent advances in the chemistry and biology of folypoly-γ-glutamate synthetase substrates and inhibitors. Curr. Med. Chem.-Anti-Cancer Agents 2002;2:331-355.
4. Zhao R and Goldman ID: Resistance to antifolates. Oncogene 2003;22:7431-7457.
5. Lu K, Yin M-B, McGuire JJ, Bonmassar E, and Rustum YM: Mechanisms of resistance to N-[5-[N-(3. 4-Dihydro-2methyl-4-oxoquinazolin-6-ylmethyl)-N- methylamino]-2-thenoyl]-L-glutamic acid (ZD1694), a folate based thymidylate synthase inhibitor, in the HCT-8 human ileocecal adenocarcinoma cell line. Biochem Pharmacol 1995;50:391-398.
6. Leclerc GJ and Barredo JC: Folypoly-γ-glutamate synthetase gene mRNA splice variants and protein expression in primary human leukemia cells, cell lines, and normal human tissues. Clin Cancer Res 2001;7:942-951.
7. Sohn K-J, Smirnakis F, Moskovitz DN, Novakovic P, Yates Z, Lucock M, Croxford R, and Kim Y-I: Effects of folypolyglutamate synthetase modulation on chemosensitivity of colon cancer cells to 5-fluorouracil and methotrexate. Gut 2004;53:1825-1831.
8. Liani E, Rothem L, Bunni MA, Smith CA, Jansen G, and Assaraf YG: Loss of folypoly-γ-glutamate synthetase activity is a dominant mechanism of resistance to polyglutamylation-dependent novel antifolates in multiple human leukemia sublines, hit J Cancer 2003;103:587-599.
9. Mauritz R, Peters GJ, Priest DG, Assaraf YG, Drori S, Kathmann I, Noordhuis P, Bunni MA, Rosowsky A, Schornagel JH, Pinedo HM, and Jansen G: Multiple mechanisms of resistance to methotrexate and novel antifolates in human CCRF-CEM leukemia cells and their implications for folate homeostasis. Biochem Pharmacol 2002;63:105-115.
10. Kim J-H, Lee K-W, Jung Y, Kim TY, Ham HS, Jong H-S, Jung KH, Im S-A, Kim T-Y, Kim NK, and Bang YJ: Cytotoxic effects of pemetrexed in gastric cancer cells. Cancer Sci 2005; 96:365-371.
11. Dotzlaf J, Carpenter J, Luo S, Roberts EF, Solenberg PJ, Qian Y-W, Lin A, He X, Sandusky GE, McClure DB, Chen VJ, and Zuckerman SH: Derivation and characterization of a monoclonal antibody against human glycinamide ribonucleotide formyltransferase. Hybridoma 2006; 25:139-144.
12. McGuire JJ, Russell CA, and Balinska M: Human cytosolic and mitochondrial folypolyglutamate synthetase are electrophoretically distinct. Expression in antifolate-sensitive and resistant human cell lines. J Biol Chem 2000;275:13012-13016.
13. Sun X, Bognar AL, Baker EN, and Smith CA: Structural homologies with ATP- and folate-binding enzymes in the crystal structure of folypolyglutamate synthetase. Proc Natl Acad Sci USA 1998; 95:6647-6652.
14. Sun X, Cross JA, Bognar AL, Baker EN, and Smith CA: Folate-binding triggers the activation of folypolyglutamate synthetase. J Mol Biol 2001;310:1067-1078.
15. Sanghani SP, Sanghani PC, and Moran RG: Identification of three key active site residues in the C-terminal domain of human recombinant folypoly-γ-glutamate synthetase by site-directed mutagenesis. J Biol Chem 1999;274:27018-27027.