Identification of three key active site residues in the C-terminal domain of human recombinant folylpoly-γ-glutamate synthetase by site- directed mutagenesis

Journal of Biological Chemistry

Volumn 274, Issue 38, 1999, Pages 27018-27027

  Sanghani, Sonal P ^a,b   Sanghani, Paresh C ^a,b   Moran, Richard G ^a  

^a VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; COMPLEMENTARY DNA; FOLYLPOLYGLUTAMATE SYNTHASE;

ARTICLE; AUXOTROPHY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME KINETICS; HUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BINDING SITES; CATALYSIS; CONSERVED SEQUENCE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IODOACETAMIDE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE SYNTHASES; POTASSIUM; RECOMBINANT PROTEINS;

EID: 0033578752     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.38.27018     Document Type: Article

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