Functional characterization of small CTD phosphatases

Methods in Molecular Biology

Volumn 365, Issue , 2007, Pages 335-346

  Yeo, Michele ^a   Lin, Patrick S ^b  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

carboxyl terminal domain; CTD phosphatase; neuronal gene silencing; REST NRSF; RNA polymerase II; SCP

Indexed keywords

CARBOXY TERMINAL DOMAIN PHOSPHATASE; CARBOXY-TERMINAL DOMAIN PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE; RNA POLYMERASE II; TRANSCRIPTION FACTOR II; TRANSCRIPTION FACTOR IIH; TRANSCRIPTION FACTOR TFIIF; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL LINE; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; RNA INTERFERENCE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; RNA INTERFERENCE; RNA POLYMERASE II; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION FACTOR TFIIH; TRANSCRIPTION FACTORS, TFII; TRANSCRIPTION, GENETIC;

EID: 34447286247     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-267-X:335     Document Type: Article

References (23)

1. Yeo, M., Lin, P. S., Dahmus, M. E., and Gill, G. N. (2003) A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J. Biol. Chem. 278, 26,078-26,085. (Pubitemid 36835376)
2. Yeo, M., Lee, S. K., Lee, B., Ruiz, E. C., Pfaff, S. L., and Gill, G. N. (2005) Small CTD phosphatases function in silencing neuronal gene expression. Science 307, 596-600. (Pubitemid 40165685)
3. Komarnitsky, P., Cho, E. J., and Buratowski, S. (2000) Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during transcription. Genes Dev. 14, 2452-2460.
4. Orphanides, G. and Reinberg, D. (2002) A unified theory of gene expression. Cell 108, 439-451. (Pubitemid 34260870)
5. Lin, P. S., Marshall, N. F., and Dahmus, M. E. (2002) CTD phosphatase: role in RNA polymerase II cycling and the regulation of transcript elongation. Prog. Nucleic Acid Res. Mol. Biol. 72, 333-365.
6. Cho, H., Kim, T. K., Mancebo, H., Lane, W. S., Flores, O., and Reinberg, D. (1999) A protein phosphatase functions to recycle RNA polymerase II. Genes Dev. 13, 1540-1552. (Pubitemid 29297922)
7. Collet, J. F., Stroobant, V., Pirard, M., Delpierre, G., and Van Schaftingen, E. (1998) A new class of phosphotransferases phosphorylated on an aspartate residue in an amino-terminal DXDX(T/V) motif. J. Biol. Chem. 273, 14,107-14,112. (Pubitemid 28319117)
8. Lin, P. S., Dubois, M. F., and Dahmus, M. E. (2002) TFIIF-associating carboxylterminal domain phosphatase dephosphorylates phosphoserines 2 and 5 of RNA polymerase II. J. Biol. Chem. 277, 45,949-45,956. (Pubitemid 35417578)
9. Cho, E. J., Kobor, M. S., Kim, M., Greenblatt, J., and Buratowski S. (2001) Opposing effects of Ctk1 kinase and Fcp1 phosphatase at Ser 2 of the RNA polymerase II C-terminal domain. Genes Dev. 15, 3319-3329. (Pubitemid 34008196)
10. Kamenski, T., Heilmeier, S., Meinhart, A., and Cramer, P. (2004) Structure and mechanism of RNA polymerase II CTD phosphatases. Mol. Cell 5, 399-407. (Pubitemid 39092756)
11. Chong, J. A., Tapia-Ramirez, J., Kim, S., et al. (1995) REST: a mammalian silencer protein that restricts sodium channel gene expression to neurons. Cell 80, 949-957.
12. Schoenherr, C. J. and Anderson, D. J. (1995) The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes. Science 267, 1360-1363.
13. Hakimi, M. A., Bochar, D. A., Chenoweth, J., Lane, W. S., Mandel, G., and Shiekhattar, R. (2002) A core-BRAF35 complex containing histone deacetylase mediates repression of neuronal-specific genes. Proc. Natl. Acad. Sci. USA 99, 7420-7425. (Pubitemid 34568706)
14. Kokura, K., Kaul, S. C., Wadhwa, R., et al. (2001) The Ski protein family is required for MeCP2-mediated transcriptional repression. J. Biol. Chem. 276, 34,115-34,121.
15. Maile, T., Kwoczynski, S., Katzenberger, R. J., Wassarman, D. A., and Sauer, F. (2004) TAF1 activates transcription by phosphorylation of serine 33 in histone H2B. Science 304, 1010-1014. (Pubitemid 38638099)
16. Kang, M. E. and Dahmus, M. E. (1995) The photoactivated cross-linking of recombinant C-terminal domain to proteins in a HeLa cell transcription extract that comigrate with transcription factors IIE and IIF. J. Biol. Chem. 270, 23,390-23,397.
17. Hodo, H. G. and Blatti, S. P. (1977) Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II. Biochemistry 16, 2334-2343. (Pubitemid 8123516)
18. Kang, M, E. and Dahmus, M. E. (1993) RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter. J. Biol. Chem. 268, 25,033-25,040.
19. Wang, B. Q., Lei, L., and Burton, Z. F. (1994) Importance of codon preference for production of human RAP74 and reconstitution of the RAP30/74 complex. Protein Express. Purif. 5, 476-485. (Pubitemid 2145877)
20. Payne, J. M. and Dahmus, M. E. (1993) Partial purification and characterization of two distinct protein kinases that differentially phosphorylate the carboxylterminal domain of RNA polymerase subunit IIa. J. Biol. Chem. 268, 80-87. (Pubitemid 23021288)
21. Chambers, R. S. and Dahmus, M. E. (1994) Purification and characterization of a phosphatase from HeLa cells which dephosphorylates the C-terminal domain of RNA polymerase II. J. Biol. Chem. 269, 26,243-26,248. (Pubitemid 24328440)
22. Chambers, R. S., Wang, B. Q., Burton, Z. F., and Dahmus, M. E. (1995) The activity of COOH-terminal domain phosphatase is regulated by a docking site on RNA polymerase II and by the general transcription factors IIF and IIB. J. Biol. Chem. 270, 14,962-14,969.
23. Lin, P. S. and Dahmus, M. E. (2003) Dephosphorylation of the carboxyl-terminal domain of RNA polymerase II. Methods Enz. 370, 155-165. (Pubitemid 37542776)