Spatial distribution of actin and tubulin in human sperm nuclear matrix-intermediate filament whole mounts - A new paradigm

Microscopy Research and Technique

Volumn 70, Issue 7, 2007, Pages 589-598

  Kadam, Kaushiki ^a   D'Souza, Serena ^a   Natraj, Usha ^a  

^a NATIONAL INSTITUTE FOR RESEARCH IN REPRODUCTIVE HEALTH   (India)

Author keywords

Actin; NM IF; Unembedded sperm; tubulin

Indexed keywords

ACETAL RESINS; ELECTRONS; PROTEINS;

ACTIN; DIFFERENTIATED CELLS; FREE ELECTRON; HUMAN SPERM; INTERMEDIATE FILAMENTS; LOCALISED; NUCLEAR MATRIX; NUCLEAR MATRIX-INTERMEDIATE FILAMENT; UNEMBEDDED SPERM; Β-TUBULIN;

SPATIAL DISTRIBUTION;

ACTIN; CYTOSKELETON PROTEIN; GLUTARALDEHYDE; MYOSIN IIA; TUBULIN;

ANTIGENICITY; ARTICLE; CELL DIFFERENTIATION; CELL NUCLEUS MATRIX; CELL ULTRASTRUCTURE; CELLULAR DISTRIBUTION; CENTRIOLE; CORRELATION ANALYSIS; CYTOSKELETON; ELECTRON MICROSCOPY; EMBEDDING; FERTILIZATION; HUMAN; HUMAN CELL; IMMUNOGOLD ELECTRON MICROSCOPY; IMMUNOREACTIVITY; INTERMEDIATE FILAMENT; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SEMEN ANALYSIS; SUPRAMOLECULAR CHEMISTRY;

EID: 34447257405     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20438     Document Type: Article

References (30)

1. Bendayan M, Roth J, Perrelet A, Orci L. 1980. Quantitative immunocytochemical localisation of pancreatic secretory proteins, in subcellular compartments of the rat acinar cells. J Histochem Cytochem 28:149-160.
2. Brathauer GL. 1994. Immunocytochemical methods and protocols. In: Javois LC, editor. Methods in molecular Biology, Vol. 34. Totowa, NJ: Humana Press. p. 97.
3. Breitbart N, Cohen G, Rubintein S. 2005. Role of actin cytoskeleton in mammalian sperm capacitation and the acrosome reaction. Reproduction 129:263-268.
4. Brener E, Rubinstein S, Cohen G, Shternall K, Rivlin J, Breitbart H. 2003. Remodeling of the actin cytoskeleton during mammalian sperm capacitation and acrosome reaction. Biol Reprod 68:837-845.
5. Brorson SH. 2002. pH-dependent effect of heat-induced antigen retrieval of epoxy section for electron microscopy. Micron 33:481-482.
6. Cabello-Agueros JF, Hernandez-Gonzalez EO, Mujica A. 2003. The role of F-actin cytoskeleton-associated gelsolin in the guinea pig capacitation and acrosome reaction. Cell Motil Cytoskeleton 56:94-108.
7. Campanella C, Gabbiani G, Bacceti B, Burrini AG, Pallini V. 1979. Actin and myosin in the vertebrate acrosomal region. J Submicrosc Cytol 11:53-71.
8. Casale A, Camatini M, Skalli O, Gabbiani G. 1988. Characterisation of actin isoforms in ejaculated boar spermatozoa. Gamete Res 20:133-144.
9. Causton BE. 1984. The choice of resins for electron immunocytochemistry. In: Polak JM, Varendell IM, editors. Immunolabelling for electron microscopy. The Netherlands: Elsevier. Chapter 3. p. 32.
10. Clarke GN, Clarke FM, Wilson S. 1982. Actin in human spermatozoa. Biol Reprod 26:319-327.
11. Fey EG, Krochmalnic G, Penman S. 1986. The nonchromatin substructures of the nucleus: The ribonucleoprotein (RNP)-containing and RNP-depleted matrices analysed by sequential fractionation and resinless section electron microscopy. J Cell Biol 102:1654-1665.
12. Flaherty SP, Winfrey VP, Olson GE. 1988. Localisation of actin in human, bull, rabbit and hamster sperm by immunoelectron microscopy. Anat Rec 221:599-610.
13. Hernández-González EO, Lecona-Valera AN, Escobar-Herrera J, Mujica A. 2000. Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa. Cell Motil Cytoskeleton 46:43-58.
14. Jamil K. 1984. Plasma membrane cytoskeletal complex of the mammalian spermatozoa. Arch Androl 13:177-193.
15. Kadam KM, D'Souza SJ, Bandivdekar AH, Natraj U. 2006. Identification and characterisation of oviductal glycoprotein binding protein partner on gametes: Epitopic similarity to non muscle myosin IIA. Mol Hum Reprod 12:275-282.
16. Liu DY, Martic M, Clarke GN, Grkovic I, Garrett C, Dunlop ME, Baker HW. 2002. An anti-actin monoclonal antibody inhibits the zona pellucid-induced acrosome reaction and hyperactivated motility of human spermatozoa. Mol Hum Reprod 8:37-47.
17. Liu DY, Clarke GN, Baker HW. 2005. Exposure of actin on the surface of the human sperm head during in vitro culture relates to sperm morphology, capacitation and zona binding. Hum Reprod 20:999-1005.
18. Lopata A, Patulla MJ, Chang A, James B. 1976. A method for collecting motile spermatozoa from human semen. Fertil Steril 27:677-689.
19. Markova MD. 2004. Electron microscopic observations of human sperm whole-mounts after extraction for nuclear matrix and intermediate filaments (NM-IF). Int J Androl 27:291-295.
20. Nickerson JA, Krockmalnic G, He D, Penman S. 1990. Immunolocalization in three dimensions: Immunogold staining of cytoskeletal and nuclear matrix proteins in resinless electron microscopy sections. Proc Natl Acad Sci USA 87:2259-2263.
21. Ocampo J, Mondragón R, Roa-Espitia AL, Chiquete-Félix N, Salgado ZO, Mújica A. 2005. Actin, myosin, cytokeratins and spectrin are components of the guinea pig sperm nuclear matrix. Tissue Cell 37:293-308.
22. Penman S. 1995. Rethinking cell structure. Proc Natl Acad Sci USA 92:5251-5257.
23. Phillips C, Yamakawa K, Adelstein RS. 1995. Cloning of the cDNA encoding human nonmuscle myosin heavy chain-B and analysis of human tissues with isoform-specific antibodies. J Muscl Res Cell Motil 16:379-389.
24. Prigent Y, Kann ML, Lach-Gar H, Péchart I, Fouquet JP. 1996. Glutamylated tubulin as a marker for microtubule heterogeneity in human sperm flagellum. Mol Hum Reprod 2:573-581.
25. Shi S-R, Cote RJ, Taylor CR. 1997. Antigen retrieval immunohistochemistry: Past, present, and future. J Histochem Cytochem 45:327-344.
26. Solberg LB, Melhus G, Brorson SH, Wendel M, Reinholt FP. 2006. Heat-induced retrieval of immunogold labeling for nucleobindin and osteoadherin from Lowicryl sections of bone. Micron 37:347-354.
27. Virtanen I, Badley RA, Paasivuo R, Lehto V-P. 1984. Distinct cytoskeletal domains revealed in sperm cells. J Cell Biol 99:1083-1091.
28. Vogl AW. 1989. Distribution and function of organized concentrations of actin filaments of mammalian spermatogeic cells and Sertoli cells. Intl Rev Cytol 119:1-56.
29. Wolf JP, Feneux D, Ducot B, Rodrigues D, Jouannet P. 1995. Influence of sperm movement parameters on human sperm-oolemma fusion. J Reprod Fertil 105:185-192.
30. World Health Organisation. 1999. WHO laboratory manual for examination of human semen and cervical mucus interaction, 4th ed. Cambridge, UK: Cambridge University Press.