RNA interference silencing of chalcone synthase, the first step in the flavonoid biosynthesis pathway, leads to parthenocarpic tomato fruits

Plant Physiology

Volumn 144, Issue 3, 2007, Pages 1520-1530

  Schijlen, Elio G W M ^a   De Vos, C H Ric ^a   Martens, Stefan ^b   Jonker, Harry H ^a   Rosin, Faye M ^a   Molthoff, Jos W ^a   Tikunov, Yury M ^a   Angenent, Gerco C ^a   Van Tunen, Arjen J ^c   Bovy, Arnaud G ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c KEYGENE N V   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; ENZYMES; FORESTRY;

CHALCONE SYNTHASE (CHS); PARTHENOCARPIC TOMATOES;

RNA;

BIOSYNTHESIS; ENZYMES; FORESTRY; NUCLEIC ACIDS;

LYCOPERSICON ESCULENTUM; SOLANUM;

EID: 34447132250     PISSN: 00320889     EISSN: 15322548     Source Type: Journal    
DOI: 10.1104/pp.107.100305     Document Type: Article

References (51)

1. Abad M, Monteiro AA (1989) The use of auxins for the production of greenhouse tomatoes in mild winter conditions: a review. Sci Hortic (Amsterdam) 38: 167-192
2. Brown DE, Rashotte AM, Murphy AS, Normanly J, Tague BW, Peer WA, Taiz L, Muday GK (2001) Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis. Plant Physiol 126: 524-535
3. Bovy A, de Vos R, Kemper M, Schijlen E, Almenar Pertejo M, Muir S, Collins G, Robinson S, Verhoeyen M, Hughes S, et al (2002) High-flavonol tomatoes resulting from heterologous expression of the maize transcription factor gene Lc and C1. Plant Cell 14: 2509-2526
4. Bovy A, van den Berg C, de Vrieze G, Thompson WF, Weisbeek P, Smeekens S (1995) Light-regulated expression of the Arabidopsis thaliana ferredoxin gene requires sequences upstream and downstream of the transcription initiation site. Plant Mol Biol 27: 27-39
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254
6. Burbulis IE, Iacobucci M, Shirley BW (1996) A null mutation in the first enzyme of flavonoid biosynthesis does not affect male fertility in Arabidopsis. Plant Cell 8: 1013-1025
7. Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19-21
8. Deveaux Y, Peaucelle A, Roberts GR, Coen E, Simon R, Mizukami Y, Traas J, Murray JA, Doonan JH, Laufs P (2003) The ethanol switch: a tool for tissue-specific gene induction during plant development. Plant J 36: 918-930
9. Edlund AF, Swanson R, Preuss D (2004) Pollen and stigma structure and function: the role of diversity in pollination. Plant Cell (Suppl) 16: S84-S97
10. Falavigna A, Baldino M, Soressi GP (1978) Potential of the mono Mendelian factor pat in tomato breeding for industry. Genet Agrar 32: 159-160
11. Fillatti JJ, Kiser J, Rose R, Comai L (1987) Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/Technol 5: 726-730
12. Fischer R, Budde I, Hain R (1997) Stilbene synthase gene expression causes changes in flower colour and male sterility in tobacco. Plant J 11: 489-498
13. Fos M, Nuez F, García-Martínez JL (2000) The pat-2 gene, which induces natural parthenocarpy, alters gibberellin content in unpollinated tomato ovaries. J Plant Physiol 122: 471-479
14. Fos M, Proaño K, Nuez F, García-Martínez JL (2001) Role of gibberellins in parthenocarpic fruit development induced by the genetic system pat-3/ pat-4 in tomato. Physiol Plant 111: 545-550
15. Giovinazzo G, D'Amico L, Paradiso A, Bollino R, Sparvoli F, DeGara L (2005) Antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene. Plant Biotechnol J 3: 57-69
16. Gorguet B, van Heusden AW, Lindhout P (2005) Parthenocarpic fruit development in tomato. Plant Biol 7: 131-139
17. Gustafson FG (1942) Parthenocarpy: natural and artificial. Bot Rev 8: 599-654
18. Gynheung AN, Ebert PR, Mitra A, Ha SB (1988) Binary vectors. In S Gelvin, R Schilperoort, D Verma, eds, Plant Molecular Biology Manual. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp A3/1-19
19. Hunt JM, Baker EA (1980) Phenolic constituents of tomato fruit cuticles. Phytochemistry 19: 1415-1419
20. Jackson AL, Linsley PS (2004) Noise amidst the silence: off-target effects of siRNAs? Trends Genet 20: 521-524
21. Jorgensen RA, Dooner HK (1986) Activity of anthocyanin biosynthetic enzymes and CHS in anthocyanin deficient mutants in tomato. Tomato Genet Coop Rep 36: 6-7
22. Koes RE, Quattrocchio F, Mol JNM (1994) The flavonoid biosynthetic pathway in plants: function and evolution. Bioessays 16: 123-132
23. Kurtz S, Choudhuri JV, Ohlebusch E, Schleiermacher C, Stoye J, Giegerich R (2001) REPuter: the manifold applications of repeat analysis on a genomic scale. Nucleic Acids Res 29: 4633-4642
24. Mascarenhas JP (1993) Molecular mechanisms of pollen tube growth and differentiation. Plant Cell 5: 1303-1314
25. Mazzucato A, Olimpieri I, Ciampolini F, Cresti M, Soressi GP (2003) A defective pollen-pistil interaction contributes to hamper seed set in the parthenocarpic fruit tomato mutant. Sex Plant Reprod 16: 157-164
26. Mo Y, Nagel C, Taylor LP (1992) Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc Natl Acad Sci USA 89: 7213-7217
27. Mol J, Grotewold E, Koes R (1998) How genes paint flowers ands seeds. Trends Plant Sci 3: 212-217
28. Muir SR, Collins GJ, Robinson S, Hughes S, Bovy S, De Vos CH, van Tunen AJ, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19: 470-474
29. Napoli CA, Fahy D, Wang HY, Taylor LP (1999) White anther: a Petunia mutant that abolishes pollen flavonol accumulation, induces male sterility, and is complemented by a chalcone synthase transgene. Plant Physiol 120: 615-622
30. Noda K, Glover BJ, Linstead P, Martin C (1994) Flower colour intensity depends on specialized cell shape controlled by a Myb-related transcription factor. Nature 369: 661-664
31. Nuez F, Costa J, Cuartero J (1986) Genetics of the parthenocarpy for tomato varieties 'Sub-Arctic Plenty', '75/59' and 'Severianin'. Z Pflanzenzüchtung 96: 200-206
32. O'Neill SD, Tong Y, Sporlein B, Forkmann G, Yoder YI (1990) Molecular genetic analysis of chalcone synthase in Lycopersicon esculentum and an anthocyanin-deficient mutant. Mol Gen Genet 224: 279-288
33. Philouze J, Maisonneuve B (1978) Heredity of the natural ability to set parthenocarpic fruits in the Soviet variety Severianin. Tomato Genet Coop Rep 28: 12-13
34. Rotino GL, Perri E, Zottini M, Sommer H (1997) Genetic engineering of parthenocarpic plants. Nat Biotechnol 15: 1398-1401
35. Ruiz JJ, Alonso A, Garcia-Martinez S, Valer M, Blasco P, Ruiz-Bevia F (2005) Quantitative analysis of flavour volatiles detects differences among closely related traditional cultivars of tomato. J Sci Food Agric 85: 54-60
36. Sastry K, Muir R (1963) Gibberellin: effect on diffusible auxin in fruit development. Science 140: 494-495
37. Schijlen E, de Vos CHR, Jonker H, van den Broeck H, Molthoff J, van Tunen A, Martens S, Bovy A (2006) Pathway engineering for healthy phytochemicals leading to the production of novel flavonoids in tomato fruit. Plant Biotechnol J 4: 433-444
38. Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D (2006) Highly specific gene silencing by artificial microRNAs in Arabidopsis. Plant Cell 18: 1121-1133
39. Subramanian S, Graham MY, Yu O, Graham TL (2005) RNA interference of soybean isoflavone synthase genes leads to silencing in tissue distal to the transformation site and to enhanced susceptibility to Phytophthora sojae. Plant Physiol 137: 1345-1353
40. Taylor LP, Jorgensen R (1992) Conditional male fertility in chalcone synthase-deficient Petunia. J Hered 83: 11-17
41. Tikunov Y, Lommen A, de Vos CHR, Verhoeven HA, Bino RJ, Hall RD, Bovy AG (2005) A novel approach for nontargeted data analysis for metabolomics. Large-scale profiling of tomato fruit volatiles. Plant Physiol 139: 1125-1137
42. Van der Meer IM, Stam ME, Van Tunen AJ, Mol JN, Stuitje AR (1992) Antisense inhibition of flavonoid biosynthesis in Petunia anthers results in male sterility. Plant Cell 4: 253-262
43. van Houwelingen A, Souer E, Spelt K, Kloos D, Mol J, Koes R (1998) Analysis of flower pigmentation mutants generated by random transposon mutagenesis in Petunia hybrida. Plant J 13: 39-50
44. Varoquaux F, Blanvillain R, Delseny M, Gallois P (2000) Less is better; new approaches for seedless fruit production. Trends Biotechnol 18: 233-242
45. Wang H, Jones B, Li Z, Frasse P, Delalande C, Regad F, Chaabouni S, Latche A, Pech J-C, Bouzayen M (2005) The tomato Aux/IAA transcription factor IAAg is involved in fruit development and leaf morphogenesis. Plant Cell 17: 2676-2692
46. Wasson AP, Pellerone FI, Mathesius U (2006) Silencing the flavonoid pathway in Medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by Rhizobia. Plant Cell 18: 1617-1629
47. Wiermann R (1979) Stage specific phenylpropanoid metabolism during pollen development. In M Luckner, L Schreiber, eds, Regulation of Secondary Product and Plant Hormone Metabolism. Pergamon Press, Oxford, pp 231-239
48. Yilmaz E (2001) The chemistry of fresh tomato flavor. Turk J Agric For 25: 149-155
49. Ylstra B, Muskens M, van Tunen AJ (1996) Flavonols are not essential for fertilization in Arabidopsis thaliana. Plant Mol Biol 32: 1155-1158
50. Ylstra B, Touraev A, Benito Moreno RM, Stöger E, van Tunen AJ, Vicente O, Mol JNM, Heberle-Bors E (1992) Flavonols stimulate development, germination and tube growth of tobacco pollen. Plant Physiol 100: 902-907
51. Yoder JI, Belzile F, Tong Y, Goldsbrough A (1994) Visual markers for tomato derived from the anthocyanin biosynthetic pathway. Euphytica 79: 163-167