Reaction mechanism of glutathione synthetase from Arabidopsis thaliana: Site-directed mutagenesis of active site residues

Journal of Biological Chemistry

Volumn 282, Issue 23, 2007, Pages 17157-17165

  Herrera, Katherine ^a,b   Cahoon, Rebecca E ^a   Kumaran, Sangaralingam ^a   Jez, Joseph M ^a  

^a DONALD DANFORTH PLANT SCIENCE CENTER   (United States)

^b INSTITUTE OF ECONOMIC BOTANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE TITRATION; GLUTAMYLCYSTEINE; GLUTATHIONE; INTRACELLULAR REDOX ENVIRONMENT;

ADENOSINETRIPHOSPHATE; AMINO ACIDS; BINDING SITES; MUTAGENESIS; NUCLEOTIDES; PLANTS (BOTANY); POLYPEPTIDES; REACTION KINETICS;

ENZYME ACTIVITY;

ADENOSINE TRIPHOSPHATE; ARGININE; DEUTERIUM; GAMMA GLUTAMYLCYSTEINE; GLUTAMIC ACID; GLUTAMINE; GLUTATHIONE; GLUTATHIONE SYNTHASE; GLYCINE; MAGNESIUM ION; MUTANT PROTEIN; NUCLEOTIDE; VEGETABLE PROTEIN;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CHEMICAL PARAMETERS; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INACTIVATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; EUKARYOTE; FLUORESCENCE; ISOTOPE ANALYSIS; PH; PRIORITY JOURNAL; PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; TITRIMETRY; TURNOVER TIME; WILD TYPE; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

ARABIDOPSIS THALIANA; EUKARYOTA;

ADENOSINE TRIPHOSPHATE; AMINO ACID SEQUENCE; ARABIDOPSIS; BINDING SITES; GLUTATHIONE SYNTHASE; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 34447125699     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M700804200     Document Type: Article

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