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Volumn 17, Issue 15, 2007, Pages 4135-4139

Natural feruloyl monoglyceride macrocycles as protecting factors against free-radical damage of lipidic membranes

Author keywords

Antioxidant activity; Carex distachya; Feruloyl monoglyceride macrocycles; Lipidic membranes; Spectroscopic analysis

Indexed keywords

ACYLGLYCEROL; CAREX DISTACHYA EXTRACT; FERULOYL MONOGLYCERIDE; FREE RADICAL; HERBACEOUS AGENT; MACROCYCLIC COMPOUND; UNCLASSIFIED DRUG;

EID: 34347396770     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.05.047     Document Type: Article
Times cited : (8)

References (14)
  • 5
    • 34347403121 scopus 로고    scopus 로고
    • note
    • Plants of Carex distachya Desf. (Cyperaceae) were collected in June 2004 in Castel Volturno, near Caserta (Campania, Italy), and identified by Dr. Assunta Esposito of the Department of Scienze della Vita of Second University of Naples (SUN). A voucher specimen has been deposited at the herbarium of the Dipartimento di Scienze della Vita of SUN.
  • 8
    • 34347400298 scopus 로고    scopus 로고
    • note
    • 2O (5:4:1), gave pure compounds 1-9.
  • 9
    • 34347390538 scopus 로고    scopus 로고
    • note
    • +,...
  • 11
    • 3042698412 scopus 로고    scopus 로고
    • The assay of superoxide radical scavenging activity was based on the capacity of each isolated metabolite (0.1 mg/ml) to inhibit the photochemical reduction of nitroblue tetrazolium (NBT) in the riboflavin-light-NBT system. Each 3 ml reaction mixture contained 50 mM sodium phosphate buffer (pH 7.8), 13 mM methionine, 2 μM riboflavin, 100 μM EDTA, 75 μM NBT and 100 μl sample solution. The production was followed by monitoring the increase in absorbance at 560 nm after 10 min illumination from a fluorescent lamp
    • Nabasree D., and Bratati D. Food Chem. 88 (2004) 219 The assay of superoxide radical scavenging activity was based on the capacity of each isolated metabolite (0.1 mg/ml) to inhibit the photochemical reduction of nitroblue tetrazolium (NBT) in the riboflavin-light-NBT system. Each 3 ml reaction mixture contained 50 mM sodium phosphate buffer (pH 7.8), 13 mM methionine, 2 μM riboflavin, 100 μM EDTA, 75 μM NBT and 100 μl sample solution. The production was followed by monitoring the increase in absorbance at 560 nm after 10 min illumination from a fluorescent lamp
    • (2004) Food Chem. , vol.88 , pp. 219
    • Nabasree, D.1    Bratati, D.2
  • 12
    • 34347403990 scopus 로고    scopus 로고
    • note
    • Preparation of liver microsomes. Microsomes were isolated from male Wistar rats (250 g). Animals were killed by decapitation. The liver was quickly excised and washed several times with ice cold saline solution (0.15 M KCl, pH 7.4), then homogenized in the same saline solution. The homogenate was filtered and then centrifuged successively at 1600g for 10 min. The supernatants were followed by centrifugation at 20,000g for 10 min and the pellets were discarded. Microsomes were obtained from the 20,000 g supernatant by centrifugation at 105,000g for 1 h at 4 °C. The microsomal pellet was washed by suspension in cold ice Tris-buffer (pH 7.4) and then stored at -80 °C.
  • 13
    • 0019751507 scopus 로고
    • The thiobarbituric acid assay was carried out according to the method reported by Markwell et al., with some modifications. The liver microsomes (10 μl), containing 100 μg protein, were emulsified with 15 mg of Tween 40 previously dissolved in 1 ml of phosphate buffer (0.2 M, pH 7.4). The emulsion was incubated for 3 h at 60 °C with 100 μl of water solution of test compounds (0.1 mg/ml). Then the reaction mixture was treated with 2 ml of TBA reagent consisting of 0.37% of thiobarbituric acid, 15% of trichloroacetic acid in 0.5 N HCl and placed in a boiling water bath for 1 h, cooled and centrifuged using a Beckman GS-15R centrifuge for 5 min at 3500g. The supernatant was measured at 532 nm. Inhibition of lipid peroxidation was measured as percentage vs blank containing no test compounds.
    • Markwell M.A., Haas S.M., Tolbertr N.E., and Bieber L.L. Methods Enzymol. 72 (1981) 296 The thiobarbituric acid assay was carried out according to the method reported by Markwell et al., with some modifications. The liver microsomes (10 μl), containing 100 μg protein, were emulsified with 15 mg of Tween 40 previously dissolved in 1 ml of phosphate buffer (0.2 M, pH 7.4). The emulsion was incubated for 3 h at 60 °C with 100 μl of water solution of test compounds (0.1 mg/ml). Then the reaction mixture was treated with 2 ml of TBA reagent consisting of 0.37% of thiobarbituric acid, 15% of trichloroacetic acid in 0.5 N HCl and placed in a boiling water bath for 1 h, cooled and centrifuged using a Beckman GS-15R centrifuge for 5 min at 3500g. The supernatant was measured at 532 nm. Inhibition of lipid peroxidation was measured as percentage vs blank containing no test compounds.
    • (1981) Methods Enzymol. , vol.72 , pp. 296
    • Markwell, M.A.1    Haas, S.M.2    Tolbertr, N.E.3    Bieber, L.L.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.