Possible biohazard risk from infectious tissue and culture cells preserved with RNAlater™ [4]

Clinical Chemistry

Volumn 53, Issue 7, 2007, Pages 1389-1390

  Kurth, Andreas ^a  

^a ROBERT KOCH INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER;

ANIMAL TISSUE; BIOSAFETY; CELL CULTURE; CHICK EMBRYO; CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; INFECTION RISK; LETTER; NONHUMAN; NUCLEOTIDE SEQUENCE; RISK ASSESSMENT; RNA ANALYSIS; TISSUE CULTURE; VACCINIA VIRUS; VIRUS INFECTIVITY; VIRUS TRANSMISSION;

ANIMALS; BUFFERS; CELL LINE, TUMOR; CHICK EMBRYO; CONTAINMENT OF BIOHAZARDS; FIXATIVES; HUMANS; RNA STABILITY; RNA, VIRAL; SPECIMEN HANDLING; TEMPERATURE; TIME FACTORS; VACCINIA VIRUS;

EID: 34347390431     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/clinchem.2007.085894     Document Type: Letter

References (6)

1. Mutter GL, Zahrieh D, Liu C, Neuberg D, Finkelstein D, Baker HE, et al. Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays. BMC Genomics 2004;5:88.
2. Paska C, Bogi K, Szilak L, Tokes A, Szabo E, Sziller I, et al. Effect of formalin, acetone, and RNAlater fixatives on tissue preservation and different size amplicons by real-time PCR from paraffin-embedded tissue. Diagn Mol Pathol 2004;13:234-40.
3. Blacksell SD, Khounsy S, Westbury HA. The effect of sample degradation and RNA stabilization on classical swine fever virus RT-PCR and ELISA methods. J Virol Methods 2004;118:33-7.
4. Keele BF, Van HF, Li Y, Bailes E, Takehisa J, Santiago ML, et al. Chimpanzee reservoirs of pandemic and nonpandemic HIV-1. Science 2006;313:523-6.
5. Uhlenhaut C, Kracht M. Viral infectivity is maintained by an RNA protection buffer. J Virol Methods 2005;128:189-91.
6. Nitsche A, Ellerbrok H, Pauli G. Detection of orthopoxvirus DNA by real-time PCR and identification of variola virus DNA by melting analysis. J Clin Microbiol 2004;42:1207-13.