Emulsion PCR-based method to detect Y chromosome microdeletions

Analytical Biochemistry

Volumn 367, Issue 2, 2007, Pages 173-178

  Ge, Qinyu ^a   Liu, Zhaobin ^b   Bai, Yunfei ^b   Zhang, Dingdong ^b   Yu, Pinfei ^a   Lu, Zuhong ^a,b  

^a Southeast University   (China)

^b SOUTHEAST UNIVERSITY   (China)

Author keywords

Emulsions; Multiplex PCR; Y chromosome microdeletions

Indexed keywords

CLINICAL RESEARCH; DIAGNOSIS; EMULSIFICATION; EMULSIONS; PLANTS (BOTANY); POLYMERASE CHAIN REACTION;

ACADEMIC RESEARCH; DIAGNOSTIC TOOLS; MALE INFERTILITY; MULTIPLEX PCR; PLASMA SAMPLES; PREGNANT WOMAN; SEQUENCE-TAGGED SITES; Y CHROMOSOMES;

CHROMOSOMES;

ARTICLE; CHROMOSOME DELETION; CLINICAL ARTICLE; CONTROLLED STUDY; EMULSION; FEMALE; HUMAN; MALE; MALE INFERTILITY; POLYMERASE CHAIN REACTION; PREGNANT WOMAN; PRIORITY JOURNAL; SEMEN ANALYSIS; SENSITIVITY ANALYSIS; SEQUENCE TAGGED SITE; Y CHROMOSOME;

EID: 34347213700     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.05.008     Document Type: Article

References (20)

1. Krausz C., Quintana-Murci L., and McElreavey K. Prognostic value of Y deletion analysis: What is the clinical prognostic value of Y chromosome microdeletion analysis?. Hum. Reprod. 15 (2000) 1431-1434
2. Pryor J.L., Kent-First M., Muallem A., Van Bergen A.H., Nolten W.E., Meisner L., and Roberts K.P. Microdeletions in the Y chromosome of infertile men. N. Engl. J. Med. 336 (1997) 534-539
3. Vogel T., Speed R.M., Teague P., and Cooke H.J. Mice with Y chromosome deletion and reduced Rbm genes on a heterozygous Dazl1 null background mimic a human azoospermic factor phenotype. Hum. Reprod. 14 (1999) 3023-3029
4. Vogt P.H., Edelmann A., Kirsch S., Henegariu O., Hirschmann P., Kiesewetter F., Kohn F.M., Schill W.B., Farah S., Ramos C., Hartmann M., Hartschuh W., Meschede D., Behre H.M., Castel A., Nieschlag E., Weidner W., Grone H.J., Jung A., Engel W., and Haidl G. Human Y chromosome azoospermia factors (AZF) mapped to different subregions in Yq11. Hum. Mol. Genet. 5 (1996) 933-943
5. Medica I., Gligorievska N., Prenc M., and Peterlin B. Y microdeletions in the Istria County, Croatia. Asian J. Androl. 7 (2005) 213-216
6. Vandamme P., Pot B., Gillis M., de Vos P., Kersters K., and Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol. Rev. 60 (1996) 407-438
7. Brownie J., Shawcross S., Theaker J., Whitcombe D., Ferrie R., Newton C., and Little S. The elimination of primer-dimer accumulation in PCR. Nucleic Acids Res. 25 (1997) 3235-3241
8. Holmberg K. Organic reactions in microemulsions. Curr. Opin. Colloid Interface Sci. 8 (2003) 187-196
9. Hori M., Fukano H., and Suzuki Y. Uniform amplification of multiple DNAs by emulsion PCR. Biochem. Biophys. Res. Commun. 352 (2007) 323-328
10. Nakano M., Nakai N., Kurita H., Komatsu J., Takashima K., Katsura S., and Mizuno A. Single-molecule reverse transcription polymerase chain reaction using water-in-oil emulsion. J. Biosci. Bioeng. 99 (2005) 293-295
11. Kojima T., Takei Y., Ohtsuka M., Kawarasaki Y., Yamane T., and Nakano H. PCR amplification from single DNA molecules on magnetic beads in emulsion: Application for high-throughput screening of transcription factor targets. Nucleic Acids Res. 33 (2005) e150
12. Wetmur J.G., Kumar M., Zhang L., Palomeque C., Wallenstein S., and Chen J. Molecular haplotyping by linking emulsion PCR: Analysis of paraoxonase 1 haplotypes and phenotypes. Nucleic Acids Res. 33 (2005) 2615-2619
13. Diehl F., Li M., He Y., Kinzler K.W., Vogelstein B., and Dressman D. BEAMing: Single-molecule PCR on microparticles in water-in-oil emulsions. Nat. Methods 3 (2006) 551-559
14. Nakano M., Komatsu J., Matsuura S., Takashima K., Katsura S., and Mizuno A. Single-molecule PCR using water-in-oil emulsion. J. Biotechnol. 102 (2003) 117-124
15. Williams R., Peisajovich S.G., Miller O.J., Magdassi S., Tawfik D.S., and Griffiths A.D. Amplification of complex gene libraries by emulsion PCR. Nat. Methods 3 (2006) 545-550
16. Tawfik D.S., and Griffiths A.D. Man-made cell-like compartments for molecular evolution. Nat. Biotechnol. 16 (1998) 652-656
17. Raeymaekers L. A commentary on the practical applications of competitive PCR. Genome Res. 5 (1995) 91-94
18. Qiu X., Wu L., Huang H., McDonel P.E., Palumbo A.V., Tiedje J.M., and Zhou J. Evaluation of PCR-generated chimeras, mutations, and heteroduplexes with 16S rRNA gene-based cloning. Appl. Environ. Microbiol. 67 (2001) 880-887
19. Baccetti B., Capitani S., Collodel G., Estenoz M., Gambera L., and Piomboni P. Infertile spermatozoa in a human carrier of Robertsonian translocation 14;22. Fertil. Steril. 78 (2002) 1127-1130
20. Dada R., Gupta N.P., and Kucheria K. Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia. J. Biosci. 28 (2003) 163-168