Validation of a new seminested PCR-based detection method for Legionella pneumophila

Journal of Microbiological Methods

Volumn 70, Issue 1, 2007, Pages 214-217

  Yanez M A ^a   Barbera V M ^a   Catalan V ^a  

^a LABAQUA SA   (Spain)

Author keywords

Environmental samples; Legionella pneumophila; Method validation; Seminesled PCR

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATION; GENE AMPLIFICATION; LEGIONELLA PNEUMOPHILA; METHODOLOGY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; CARRIER PROTEINS; LEGIONELLA PNEUMOPHILA; MEMBRANE PROTEINS; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SENSITIVITY AND SPECIFICITY; WATER MICROBIOLOGY;

LEGIONELLA PNEUMOPHILA;

EID: 34250836908     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.03.013     Document Type: Article

References (15)

1. Ballard A.L., Fry N.K., Chan L., Surman S.B., Lee J.V., Harrison T.G., and Towner K.J. Detection of Legionella pneumophila using a real-time PCR hybridization assay. J. Clin. Microbiol. 38 (2000) 4215-4218
2. Bender L., Ott M., Marre R., and Hacker J. Genome analysis of Legionella ssp. by orthogonal field alternation gel electrophoresis (OFAGE). FEMS Microbiol. Lett. 60 (1990) 253-257
3. Catalán V., Moreno C., Dasí M.A., Muñoz C., and Apraiz D. Nested polymerase chain reaction for detection of Legionella pneumophila in water. Res. Microbiol. 145 (1994) 603-610
4. Devos L., Clymans K., Boon N., and Verstraete W. Evaluation of nested PCR assays for the detection of Legionella pneumophila in a wide range of aquatic samples. J. Appl. Microbiol. 99 (2005) 916-925
5. Fiume L., Bucca M.A., and Poda G. Detection of Legionella pneumophila in water samples by species-specific real-time and nested PCR assays. Lett. Appl. Microbiol. 41 (2005) 470-475
6. Hayden R.T., Uhl J.R., Quian X., Hopkins M.K., Aubry M.C., Limper A.H., Lloyd R.V., and Cockerill F.R. Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture. J. Clin. Microbiol. 37 (2001) 2618-2626
7. Hoorfar J., Cook N., Malorny B., Wagner M., De Medici D., Abdulmawfood A., and Fach P. Making internal positive control mandatory for diagnostic PCR. J. Clin. Microbiol. 41 (2003) 5835
8. Lee T.C., Vickers R.M., Yu V.L., and Wagener M.M. Growth of 28 Legionella species on selective culture media: a comparative study. J. Clin. Microbiol. 31 (1993) 2764-2768
9. Madden T.L., Tatusov R.L., and Zhang J. Applications of network BLAST server. Methods Enzymol. 266 (1996) 131-141
10. Miyamoto H., Yamamoto H., Arima K., Fujii J., Maruta K., Isu K., Shiomori T., and Yoshida S. Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of Legionella in hospital cooling tower water. Appl. Environ. Microbiol. 63 (1997) 2489-2494
11. Rantakokko-Jalava K., and Jalava J. Development of conventional and real-time PCR assays for detection of Legionella DNA respiratory specimens. J. Clin. Microbiol. 39 (2001) 2904-2910
12. Roy C.R. The Dot/Icm transporter of Legionella pneumophila: a bacteria conductor of vesicle trafficking that orchestrates the establishment of a replicative organelle in eukaryotic hosts. Int. J. Med. Microbiol. 291 (2002) 463-467
13. Rozen S., and Skaletsky H.J. Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S., and Misener S. (Eds). Bioinformatics methods and protocols: methods in molecular biology (2000), Humana Press, Totowa, NJ 365-386. http://fokker.wi.mit.edu/primer3/ Source code available at http://fokker.wi.mit.edu/primer3/
14. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., and Higging D.H. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25 (1997) 4876-4882
15. Yáñez M.A., Carrasco-Serrano M.C., Barberá V.M., and Catalán V. Quantitative detection of Legionella pneumophila in water samples by immunomagnetic purification and real-time PCR amplification of dotA gene. Appl. Environ. Microbiol. 71 (2005) 3433-3441