A peroxidase-coupled continuous absorbance plate-reader assay for flavin monoamine oxidases, copper-containing amine oxidases and related enzymes

Nature Protocols

Volumn 1, Issue 5, 2006, Pages 2498-2505

  Holt, Andrew ^a   Palcic, Monica M ^b  

^a UNIVERSITY OF ALBERTA   (Canada)

^b CARLSBERG LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AMINE OXIDASE (COPPER CONTAINING); AMINE OXIDASE (FLAVIN CONTAINING); PEROXIDASE;

ARTICLE; METABOLISM;

AMINE OXIDASE (COPPER-CONTAINING); MONOAMINE OXIDASE; PEROXIDASE;

EID: 34250791622     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.402     Document Type: Article

References (15)

1. Childs, R.E. & Bardsley, W.G. The steady-state kinetics of peroxidase with 2,2′-azino-di-(3-ethyl- benzthiazoline-6-sulphonic acid) as chromogen. Biochem. J. 145, 93-103 (1975).
2. Szutowicz, A., Kobes, R.D. & Orsulak, P.J. Colorimetric assay for monoamine oxidase in tissues using peroxidase and 2,2′-azinodi(3-ethylbenzthiazoline-6-sulfonic acid) as chromogen. Anal. Biochem. 138, 86-94 (1984).
3. Zhou, M. & Panchuk-Voloshina, N. A one-step fluorometric method for the continuous measurement of monoamine oxidase activity. Anal. Biochem. 237, 169-174 (1997).
4. Elliott, J., Callingham, B.A. & Sharman, D.F. Semicarbazide-sensitive amine oxidases of sheep blood plasma: Changes in activity during pregnancy and alloxan-induced experimental diabetes. Res. Vet. Sci. 50, 334-339 (1991).
5. Elliott, J., Callingham, B.A. & Sharman, D.F. Amine oxidase enzymes of sheep blood vessels and blood plasma: A comparison of their properties. Comp. Biochem. Physiol. 102C, 83-89 (1992).
6. Holt, A., Sharman, D.F., Baker, G.B. & Palcic, M.M. A continuous spectrophotometric assay for monoamine oxidase and related enzymes in tissue homogenates. Anal. Biochem. 244, 384-392 (1997).
7. Di Paolo, M.L. et al. Binding of cations of group IA and IIA to bovine serum amine oxidase: Effect on the activity. Biophys. J. 83, 2231-2239 (2002).
8. Hubálek, F. et al. Demonstration of isoleucine 199 as a structural determinant for the selective inhibition of human monoamine oxidase B by specific reversible inhibitors. J. Biol. Chem. 280 15761-15766.
9. Mitra, C. & Guha, S.R. Differential activation of two monoamine oxidase types by oxygen. Experientia (Basel) 39, 270-272 (1983).
10. Nelson, D.R. & Huggins, A.K. Interference of 5-hydroxytryptamine in the assayHof glucose by glucose oxidase:pEroxidase:chromogen based methods. Anal. Biochem. 59, 46-53 (1974).
11. Fowler, C.J., Mantle, T.J. & Tipton, K.F. The nature of the inhibition of rat liver monoamine oxidase types A and B by the acetylenic inhibitors clorgyline, l-deprenyl and pargyline. Biochem. Pharmacol. 31, 3555-3562 (1982).
12. Coquil, J.F., Goridis, C., Mack, G. & Neff, N.H. Monoamine oxidase in rat arteries: Evidence for different forms and selective localization. Br. J. Pharmacol. 48, 590-599 (1973).
13. Kinemuchi, H. et al. Time-dependent inhibition of monoamine oxidase by beta-phenylethylamine. Biochem. Pharmacol. 31, 959-964 (1982).
14. Holt, A., Wieland, B. & Baker, G.B. Allosteric modulation of semicarbazide-sensitive amine oxidase activities in vitro by imidazoline receptor ligands. Br. J. Pharmacol. 143, 495-507 (2004).
15. Holt, A. et al. Identification of the quinone cofactor in mammalian semicarbazide-sensitive amine oxidase. Biochemistry 37, 4946-4957 (1998).