Development of a transformation system for gene knock-out in the flavinogenic yeast Pichia guilliermondii

Journal of Microbiological Methods

Volumn 70, Issue 1, 2007, Pages 13-19

  Boretsky, Yuriy R ^a   Pynyaha, Yuriy V ^a   Boretsky, Volodymyr Y ^a   Kutsyaba, Vasyl I ^a   Protchenko, Olga V ^b   Philpott, Caroline C ^b   Sibirny, Andriy A ^a,c  

^a INSTITUTE OF CELL BIOLOGY   (Ukraine)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c UNIVERSITY OF RZESZÓW   (Poland)

Author keywords

Codon usage; Deletion cassette; Riboflavin biosynthesis; Transformation; URA3 marker; Yeast Pichia guilliermondii

Indexed keywords

FLUOROOROTIC ACID; GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE I; IRON; LEUCINE; LITHIUM ACETATE; RIBOFLAVIN; RIBOFLAVIN SYNTHASE; URACIL;

ARTICLE; CODON; CONTROLLED STUDY; ELECTROPORATION; FUNGAL STRAIN; GENE DELETION; GENE TARGETING; GENETIC MANIPULATION; GENETIC TRANSFORMATION; HOMOLOGOUS RECOMBINATION; KNOCKOUT GENE; MARKER GENE; MOLECULAR GENETICS; MUTANT; NONHUMAN; PHENOTYPE; PICHIA; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; SPHEROPLAST FORMATION; YEAST;

CODON; DNA, FUNGAL; ELECTROPORATION; FUNGAL PROTEINS; GENE DELETION; GENETIC VECTORS; GTP CYCLOHYDROLASE; MOLECULAR BIOLOGY; MUTAGENESIS, INSERTIONAL; PICHIA; PLASMIDS; POINT MUTATION; POLYMERASE CHAIN REACTION; RIBOFLAVIN SYNTHASE; TRANSFORMATION, GENETIC;

PICHIA GUILLIERMONDII; SACCHAROMYCES CEREVISIAE;

EID: 34250719093     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.03.004     Document Type: Article

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