Molecular detection and identification of Synergistes phylotypes in primary endodontic infections

Oral Diseases

Volumn 13, Issue 4, 2007, Pages 398-401

  Siqueira Jr , J F ^a,b   Rocas I N ^a  

^a ESTÁCIO DE SÁ UNIVERSITY   (Brazil)

^b NONE   (Brazil)

Author keywords

16S rRNA gene sequencing; Apical periodontitis; Polymerase chain reaction; Root canal infection; Synergistes

Indexed keywords

GENOMIC DNA; RNA 16S;

ADULT; AMPLICON; ANAEROBIC BACTERIUM; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CLINICAL ARTICLE; CLINICAL EXAMINATION; DNA ISOLATION; ENDODONTICS; HUMAN; MOLECULAR BIOLOGY; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PERIODONTAL DISEASE; PERIODONTITIS; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SAMPLE; SPECIES IDENTIFICATION; SYNERGISTES;

BACTERIA, ANAEROBIC; BACTERIAL INFECTIONS; DENTAL PULP DISEASES; DNA, BACTERIAL; GENOME, BACTERIAL; HUMANS; MOLECULAR BIOLOGY; PERIAPICAL ABSCESS; PERIAPICAL PERIODONTITIS; PHYLOGENY; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

EID: 34250657238     PISSN: 1354523X     EISSN: 16010825     Source Type: Journal    
DOI: 10.1111/j.1601-0825.2006.01301.x     Document Type: Article

References (19)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215 : 403 410.
2. Edwards U, Rogall T, Blöcker H, Emde M, Bötter EC (1989). Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17 : 7843 7853.
3. Fouad AF, Kum KY, Clawson ML et al. (2003). Molecular characterization of the presence of Eubacterium spp. and Streptococcus spp. in endodontic infections. Oral Microbiol Immunol 18 : 249 255.
4. Godon J-J, Morinière J, Moletta M, Gaillac M, Bru V, Delgènes J-P (2005). Rarity associated with specific ecological niches in the bacterial world: the 'Synergistes' example. Environ Microbiol 7 : 213 224.
5. Hall TA (1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 : 95 98.
6. Kumar S, Tamura K, Jakobsen IB, Nei M (2001). MEGA2: molecular evolutionary genetics analysis software. Bioinformatics 17 : 1244 1245.
7. Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ (2003). New bacterial species associated with chronic periodontitis. J Dent Res 82 : 338 344.
8. Larsen N, Olsen GJ, Maidak BL et al. (1993). The Ribosomal Database Project. Nucleic Acids Res 21 : 3021 3023.
9. Lillo A, Ashley FP, Palmer RM et al. (2006). Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol Immunol 21 : 61 68.
10. Munson MA, Pitt-Ford T, Chong B, Weightman A, Wade WG (2002). Molecular and cultural analysis of the microflora associated with endodontic infections. J Dent Res 81 : 761 766.
11. Paster BJ, Boches SK, Galvin JL et al. (2001). Bacterial diversity in human subgingival plaque. J Bacteriol 183 : 3770 3783.
12. Rôças IN, Siqueira JF Jr. (2005). Detection of novel oral species and phylotypes in symptomatic endodontic infections including abscesses. FEMS Microbiol Lett 250 : 279 285.
13. Rôças IN, Siqueira JF Jr., Santos KR (2004). Association of Enterococcus faecalis with different forms of periradicular diseases. J Endod 30 : 315 320.
14. Saito D, de Toledo Leonardo R, Rodrigues JLM, Tsai SM, Hofling JF, Gonçalves RB (2006). Identification of bacteria in endodontic infections by sequence analysis of 16S rDNA clone libraries. J Med Microbiol 55 : 101 107.
15. Sakamoto M, Rôças IN, Siqueira JF, Benno Y (2006). Molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections. Oral Microbiol Immunol 21 : 112 122.
16. Siqueira JF Jr., Rôças IN (2005). Uncultivated phylotypes and newly named species associated with primary and persistent endodontic infections. J Clin Microbiol 43 : 3314 3319.
17. Siqueira JF Jr., Rôças IN, Rosado AS (2004). Investigation of bacterial communities associated with asymptomatic and symptomatic endodontic infections by denaturing gradient gel electrophoresis fingerprinting approach. Oral Microbiol Immunol 19 : 363 370.
18. Siqueira JF Jr., Rôças IN, Cunha CD, Rosado AS (2005). Novel bacterial phylotypes in endodontic infections. J Dent Res 84 : 565 569.
19. Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 : 697 703.