Molecular analysis of the NSP4 and VP6 genes of rotavirus strains recovered from hospitalized children in Rio de Janeiro, Brazil

Journal of Medical Microbiology

Volumn 56, Issue 6, 2007, Pages 854-859

  Araújo, Irene Trigueiros ^a   Heinemann, Marcos Bryan ^a   Mascarenhas, Joana D'Arc P ^b   Santos Assis, Rosane M ^a   Fialho, Alexandre Madi ^a   Leite, José Paulo G ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b INSTITUTO EVANDRO CHAGAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN VP6; PROTEIN VP7; ROTAVIRUS NONSTRUCTURAL PROTEIN 4; UNCLASSIFIED DRUG; VIRUS ANTIGEN;

ACUTE GASTROENTERITIS; AMINO ACID SEQUENCE; ARTICLE; BRAZIL; CHILD HOSPITALIZATION; GENE SEQUENCE; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PHYLOGENY; PRIORITY JOURNAL; PROTEIN FUNCTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS; VIRUS INFECTION; VIRUS ISOLATION; VIRUS MORPHOGENESIS; VIRUS STRAIN;

AMINO ACID SEQUENCE; ANTIGENS, VIRAL; BRAZIL; CAPSID PROTEINS; CHILD; CHILD, HOSPITALIZED; CLUSTER ANALYSIS; EPIDEMIOLOGY, MOLECULAR; GENOTYPE; GLYCOPROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; PHYLOGENY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; ROTAVIRUS; ROTAVIRUS INFECTIONS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; TOXINS, BIOLOGICAL; VIRAL NONSTRUCTURAL PROTEINS;

EID: 34250336905     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46787-0     Document Type: Article

References (26)

1. Araújo, I. T., Ferreira, M. S. R., Fialho, A. M., Assis, R. M., Cruz, C. M., Rocha, M. & Leite, J. P. G. (2001). Rotavirus genotypes P[4]G9, P[6]G9, and P[8]G9 in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil. J Clin Microbiol 39, 1999-2001.
2. Arista, S., Giovannelli, L., Pistoia, D., Cascio, A., Parea, M. & Gerna, G. (1990). Electropherotypes, subgroups and serotypes of human rotavirus strains causing gastroenteritis in infants and young children in Palermo, Italy, from 1985 to 1989. Res Virol 141, 435-448.
3. Ball, J. M., Tian, P., Zeng, C. Q., Morris, A. P. & Estes, M. K. (1996). Age-dependent diarrhea induced by a rotaviral nonstructural glycoprotein. Science 272, 101-104.
4. Ball, J. M., Mitchell, D. M., Gibbons, T. F. & Parr, R. D. (2005). Rotavirus NSP4: a multifunctional viral enterotoxin. Viral Immunol 18, 27-40.
5. Boom, R., Sol, C. J., Salismans, M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. & van den Noordaa, J. (1990). Rapid and simple method for the purification of nucleic acids. J Clin Microbiol 28, 495-503.
6. Ciarlet, M., Liprandi, F., Conner, M. E. & Estes, M. K. (2000). Species specificity and interspecies relatedness of NSP4 genetic groups by comparative NSP4 sequence analyses of animal rotaviruses. Arch Virol 145, 371-383.
7. Cunliffe, N. A., Woods, P. A., Leite, J. P. G., Das, B. K., Ramachandran, M., Bhan, M. K., Hart, C. A., Glass, R. I. & Gentsch, J. R. (1997). Sequence analysis of NSP4 gene of human rotavirus allows classification into two main genetic groups. J Med Virol 53, 41-50.
8. Estes, M. K. (2001). Rotaviruses and their replication. In Fields Virology, 4th edn, vol. 2, pp. 1747-1785. Edited by B. N. Fields, D. N. Knipe, P. M. Howley, R. M. Chanock, J. L. Melnick, T. P. Monath, B. Roizman & S. E. Straus. New York: Lippincott-Raven.
9. Gulati, B. R., Deepa, R., Singh, B. K. & Rao, C. D. (2006). Diversity in Indian equine rotaviruses: identification of G10, P6[1] and G10 type and a new VP7 genotype (G16) strains in diarrheic foals in India. J Clin Microbiol 45, 972-978.
10. Iturriza-Gómara, M., Isherwood, B., Desselberger, U. & Gray, J. (2001). Reassortment in vivo: driving force for diversity of human rotavirus strains isolated in the United Kingdom between 1995 and 1999. J Virol 75, 3696-3705.
11. Iturriza-Gómara, M., Wong, C., Blome, S., Desselberger, U. & Gray, J. (2002). Molecular characterization of VP6 genes of human rotavirus isolates: correlation of genogroups with subgroups and evidence of independent segregation. J Virol 76, 6596-6601.
12. Iturriza-Gómara, M., Anderton, E., Kang, G., Gallimore, C., Phillips, W., Desselberger, U. & Gray, J. (2003). Evidence for genetic linkage between the gene segments encoding NSP4 and VP6 proteins in common and reassortant human rotavirus strains. J Clin Microbiol 41, 3566-3573.
13. Kapikian, A., Hoshino, Y. & Chanock, R. (2001). Rotaviruses. In Fields Virology, 4th edn, pp. 1787-1834. Edited by B. N. Fields, D. N. Knipe, P. M. Howley, R. M. Chanock, J. L. Melnick, T. P. Monath, B. Roizman & S. E. Straus. New York: Lippincott-Raven.
14. Khamrin, P., Maneekarn, N., Peerakome, S., Chan-It, W., Yagyu, F., Okitsu, S. & Ushijima, H. (2007). Novel porcine rotavirus of genotype P[27] shares new phylogenetic lineage with G2 porcine rotavirus strain. Virology (in press).
15. Lee, C. N., Wang, Y. L., Kao, C. L., Zao, C. L., Lee, C. Y. & Chen, H. N. (2000). NSP4 gene analysis of rotaviruses recovered from infected children with and without diarrhoea. J Clin Microbiol 38, 4471-4477.
16. Leite, J. P. G., Alfieri, A. A., Woods, P. A., Glass, R. I. & Gentsch, J. R. (1996). Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis. Arch Virol 141, 2365-2374.
17. Lin, S. L. & Tian, P. (2003). Detailed computational analysis of a comprehensive set of group A rotavirus NSP4 proteins. Virus Genes 26, 271-282.
18. Martella, V., Ciarlet, M., Banyai, K., Lorusso, E., Cavalli, A., Corrente, M., Elia, G., Arista, S., Camero, M. & other authors (2006). Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain. Virology 346, 301-311.
19. Mascarenhas, J. D. P., Linhares, A. C., Bayma, A. P. G., Lima, J. C., Sousa, M. S., Araújo, I. T., Heinemann, M. B., Gusmão, R. H. P., Gabbay, Y. B. & Leite, J. P. G. (2006). Molecular analysis of VP4, VP7 and NSP4 genes of P[6]G2 rotavirus genotype strains recovered from neonates admitted to hospital in Belém, Brazil. J Med Virol 78, 281-289.
20. Mori, Y., Borgan, M. A., Ito, N., Sugiyama, M. & Minamoto, N. (2002). Diarrhea-inducing activity of avian rotavirus NSP4 glycoproteins, which differ greatly from mammalian rotavirus NSP4 glycoproteins in deduced amino acid sequence in suckling mice. J Virol 76, 5829-5834.
21. Pereira, H. G., Azeredo, R. S., Leite, J. P. G., Andrade, Z. P. & De Castro, L. (1985). A combined enzyme immunoassay for rotavirus and adenovirus (EIARA). J Virol Methods 10, 21-28.
22. Rahman, M., Matthijnssens, J., Nahar, S., Podder, G., Sack, D. A., Azim, T. & van Ranst, M. (2005). Characterization of a novel P[25]G11 human group A rotavirus. J Clin Microbiol 43, 3208-3212.
23. Tajima, F. & Nei, M. (1984). Estimation of evolutionary distance between nucleotide sequences. Mol Biol Evol 1, 269-285.
24. Tang, B., Gilbert, J. M., Matsui, S. M. & Greenberg, H. B. (1997). Comparison of the rotavirus gene 6 from different species by sequence analysis and localization of subgroup-specific epitopes using site-directed mutagenesis. Virology 237, 89-96.
25. Taylor, J. A. & Bellamy, A. R. (2003). Interaction of the rotavirus nonstructural glycoprotein NSP4 with the viral and cellular components. In Viral Gastroenteritis, pp. 225-235. Edited by U. Desselberger & J. Gray. Amsterdam: Elsevier Science.
26. Zhang, M., Zeng, C. Q., Dong, Y., Ball, J. M., Saif, L. J., Morris, A. P. & Estes, M. K. (1998). Mutations in rotavirus nonstructural glycoprotein NSP4 are associated with altered virus virulence. J Virol 72, 3666-3672.