Complementation analysis demonstrates that insulin cross-links both α subunits in a truncated insulin receptor dimer

Journal of Biological Chemistry

Volumn 282, Issue 18, 2007, Pages 13754-13758

  Shu, Jin Chan ^a   Nakagawa, Satoe ^a   Steiner, Donald F ^a,b  

^a UNIVERSITY OF CHICAGO   (United States)

^b Dept of Medicine ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTATION ANALYSIS; HOMODIMERS; TRANSIENT TRANSFECTION;

AMINO ACIDS; ASSAYS; BINDING ENERGY; CROSSLINKING; DIMERS; PROTEINS;

HORMONES;

ALANINE; DIMER; GLYCINE; INSULIN RECEPTOR; HYBRID PROTEIN; INSR PROTEIN, HUMAN; INSULIN; LEUKOCYTE ANTIGEN; PROTEIN SUBUNIT; UNCLASSIFIED DRUG;

ALPHA CHAIN; AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN HEK293; CONTROLLED STUDY; ENZYME ASSAY; HUMAN; HUMAN CELL; HYBRID CELL; IMMUNOBLOTTING; INSULIN RELEASE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; RECEPTOR AFFINITY; RECEPTOR INTRINSIC ACTIVITY; SCATCHARD PLOT; SIGNAL TRANSDUCTION; CELL LINE; CHEMISTRY; DIMERIZATION; GENE DELETION; GENETIC COMPLEMENTATION; GENETICS; HARTNUP DISEASE; METABOLISM; MISSENSE MUTATION;

AMINO ACID SEQUENCE; ANTIGENS, CD; CELL LINE; DIMERIZATION; GENETIC COMPLEMENTATION TEST; HARTNUP DISEASE; HUMANS; INSULIN; MUTATION, MISSENSE; PROTEIN BINDING; PROTEIN SUBUNITS; RECEPTOR, INSULIN; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; SIGNAL TRANSDUCTION;

EID: 34250306385     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M700724200     Document Type: Article

References (21)

1. De Meyts, P. (2004) BioEssays 26, 1351-1362
2. Andersen, A. S., Kjeldsen, T., Wiberg, F. C., Christensen, P. M., Rasmussen, J. S., Norris, K., Moller, K. B., and Moller, N. P. H. (1990) Biochemistry 29, 7363-7366
3. Schumacher, R., Soos, M. A., Schlessinger, J., Brandenburg, D., Siddle, K., and Ullrich, A. (1993) J. Biol. Chem. 268, 1087-1094
4. Kurose, T., Pashmforoush, M., Yoshimasa, Y., Carroll, R., Schwartz, G. P., Burke, G. T., Katsoyannis, P. G., and Steiner, D. F. (1994) J. Biol. Chem. 269, 29190-29197
5. Kristensen, C., Wiberg, F. C., Schaffer, L., and Andersen, A. S. (1998) J. Biol. Chem. 273, 17780-17786
6. Brandt, J., Andersen, A. S., and Kristensen, C. (2001) J. Biol. Chem. 276, 12378-12384
7. Whittaker, J., Sorensen, H., Gadboll, V. L., and Hinrichsen, J. (2002) J. Biol. Chem. 277, 47380-47384
8. Whittaker, J., and Whittaker, L. (2005) J. Biol. Chem. 280, 20932-20936
9. Ullrich, A., Bell, J. R., Chen, E. Y., Herrera, R., Petruzelli, L. M., Dull, T. J., Gray, A., Coussens, L., Liao, Y.-C., Tzubokawa, M., Mason, A., Seeburg, P. H., Grunfeld, C., Rozen, O. M., and Ramachandran, J. (1985) Nature 313, 756-761
10. Cuatrecasas, P. (1972) J. Biol. Chem. 247, 1980-1991
11. Laemmli, U. K. (1970) Nature 227, 680-685
12. Boni-Schnetzler, M., Rubin, J. B., and Pilch, P. F. (1986) J. Biol. Chem. 261, 15281-15287
13. Boni-Schnetzler, M., Scott, W., Waugh, S. M., DiBella, E., and Pilch, P. F. (1987) J. Biol. Chem. 262, 8395-8401
14. Wan, Z., Huang, K., Xu, B., Hu, S.-Q., Wang, S., Chu, Y.-C., Katsoyannis, P., and Weiss, M. A. (2005) Biochemistry 44, 5000-5016
15. de Vos, A. M., Ultsch, M., and Kossiakoff, A. A. (1992) Science 255, 306-312
16. Garrett, T. P. J., McKern, N. M., Lou, M., Elleman, T. C., Adams, T. E., Lovrecz, G. O., Zhu, H.-J., Walker, F., Frenkel, M. J., Hoyne, P. A., Jorissen, R. N., Nice, E. C., Burgess, A. W., and Ward, C. W. (2002) Cell 110, 763-773
17. Osig, H., Ishitani, R., Nureki, O., Fukai, S., Yamanaka, M., Kin, J.-H., Saito, K., Sakamoto, A., Inoue, M., Shirouzu, M., and Yokoyama, S. (2002) Cell 110, 775-787
18. Shaffer, L. (1994) Eur. J. Biochem. 221, 1127-1132
19. De Meyts, P. (1994) Diabetologia 37, Suppl. 2, S135-S148
20. Ottensmeyer, F. P., Beniac, D. R., Luo, R. Z. T., and Yip, C. C. (2002) Biochemistry 39, 12103-12112
21. McKern, N. M., Lawrence, M. C., Streltsov, V. A., Lou, M.-Z., Adams, T. E., Lovrecz, G. O., Elleman, T. C., Richards, K. M., Bently, J. D., Pilling, P. A., Hoyne, P. A., Cartledge, K. A., Pham, T. M., Lewis, J. L., Sankovich, S. E., Stoichevska, V., Da Silva, E., Robinson, C. P., Frenkel, M. J., Sparrow, L. G., Fernley, R. T., Epa, V. C., and Ward, C. W. (2006) Nature 443, 218-221