Two-photon excitation and photoconversion of EosFP in dual-color 4Pi confocal microscopy

Biophysical Journal

Volumn 92, Issue 12, 2007, Pages 4451-4457

  Ivanchenko, Sergey ^a   Glaschick, Sylvia ^a   Röcker, Carlheinz ^a   Oswald, Franz ^a   Wiedenmann, Jörg ^a   Nienhaus, G Ulrich ^a,b  

^a UNIVERSITY OF ULM   (Germany)

^b University of Illinois at Urbana Champaign   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EOSFP PROTEIN; GENE PRODUCT; UNCLASSIFIED DRUG;

ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EXCITATION; FEMALE; FLUORESCENCE; HELA CELL; HUMAN; HUMAN CELL; IMAGING; INTRACELLULAR SPACE; IRRADIATION; MITOCHONDRION; NONHUMAN; OPTICAL RESOLUTION; PHOTOACTIVATION; PHOTON; PROTEIN ANALYSIS; SPECTRAL SENSITIVITY; SPECTROSCOPY; THREE DIMENSIONAL IMAGING;

EID: 34250304369     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.106.103408     Document Type: Article

References (38)

1. Hell, S. W., and E. H. K. Stelzer. 1992. Fundamental improvement of resolution with a 4Pi-confocal fluorescence microscope using two-photon excitation. Opt. Commun. 93:277-282.
2. Egner, A., and S. W. Hell. 2005. Fluorescence microscopy with super-resolved optical sections. Trends Cell Biol. 15:207-215.
3. Gustafsson, M. G., D. A. Agard, and J. W. Sedat. 1995. Sevenfold improvement of axial resolution in 3D widefield microscopy using two objective lenses. Proc. SPIE. 2412:147-156.
4. Hofmann, M., C. Eggeling, S. Jakobs, and S. W. Hell. 2005. Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc. Natl. Acad. Sci. USA. 102:17565-17569.
5. Wiedenmann, J., and G. U. Nienhaus. 2006. Live-cell imaging with EosFP and other photoactivatable marker proteins of the GFP family. Expert Rev. Proteomics. 3:361-374.
6. Patterson, G. H., and J. Lippincott-Schwartz. 2002. A photoactivatable GFP for selective photolabeling of proteins and cells. Science. 297:1873-1877.
7. Ando, R., H. Mizuno, and A. Miyawaki. 2004. Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting. Science. 306:1370-1373.
8. Ando, R., H. Hama, M. Yamamoto-Hino, H. Mizuno, and A. Miyawaki. 2002. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA. 99:12651-12656.
9. Chudakov, D. M., V. V. Belousov, A. G. Zaraisky, V. V. Novoselov, D. B. Staroverov, D. B. Zorov, S. Lukyanov, and K. A. Lukyanov. 2003. Kindling fluorescent proteins for precise in vivo photolabeling. Nat. Biotechnol. 21:191-194.
10. Wiedenmann, J., S. Ivanchenko, F. Oswald, F. Schmitt, C. Röcker, A. Salih, K. D. Spindler, and G. U. Nienhaus. 2004. EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion. Proc. Natl. Acad. Sci. USA. 101:15905-15910.
11. Nienhaus, K., G. U. Nienhaus, J. Wiedenmann, and H. Nar. 2005. Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP. Proc. Natl. Acad. Sci. USA. 102:9156-9159.
12. Nienhaus, G. U., K. Nienhaus, A. Holzle, S. Ivanchenko, F. Renzi, F. Oswald, M. Wolff, F. Schmitt, C. Rocker, B. Vallone, W. Weidemann, R. Heilker, H. Nar, and J. Wiedenmann. 2006. Photoconvertible fluorescent protein EosFP: biophysical properties and cell biology applications. Photochem. Photobiol. 82:351-358.
13. Betzig, E., G. H. Patterson, R. Sougrat, O. W. Lindwasser, S. Olenych, J. S. Bonifacino, M. W. Davidson, J. Lippincott-Schwartz, and H. F. Hess. 2006. Imaging intracellular fluorescent proteins at nanometer resolution. Science. 313:1642-1645.
14. Hess, S. T., T. P. Girirajan, and M. D. Mason. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91:4258-4272.
15. Schneider, M., S. Barozzi, I. Testa, M. Faretta, and A. Diaspro. 2005. Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. Biophys. J. 89:1346-1352.
16. Tsutsui, H., S. Karasawa, H. Shimizu, N. Nukina, and A. Miyawaki. 2005. Semi-rational engineering of a coral fluorescent protein into an efficient highlighter. EMBO Rep. 6:233-238.
17. Ivanchenko, S., C. Röcker, F. Oswald, J. Wiedenmann, and G. U. Nienhaus. 2005. Targeted green-to-red photoconversion of EosFP, a fluorescent marker protein. J. Biol. Phys. 31:249-259.
18. Nagorni, M., and S. W. Hell. 1998. 4Pi-confocal microscopy provides three-dimensional images of the microtubule network with 100- to 150-nm resolution. J. Struct. Biol. 123:236-247.
19. Kano, H., S. Jakobs, M. Nagorni, and S. W. Hell. 2001. Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range. Ultramicroscopy. 90:207-213.
20. Egner, A., S. Jakobs, and S. W. Hell. 2002. Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast. Proc. Natl. Acad. Sci. USA. 99:3370-3375.
21. Gugel, H., J. Bewersdorf, S. Jakobs, J. Engelhardt, R. Storz, and S. W. Hell. 2004. Cooperative 4Pi excitation and detection yields sevenfold sharper optical sections in live-cell microscopy. Biophys. J. 87:4146-4152.
22. Albota, M. A., C. Xu, and W. W. Webb. 1998. Two-photon fluorescence excitation cross sections of biomolecular probes from 690 to 960 nm. Appl. Opt. 37:7352-7356.
23. Bradeley, D. J., M. H. R. Hutchinson, and H. Koetser. 1972. Interactions of picosecond laser pulses with organic molecules. II. Two-photon absorption cross-sections. Proc. R. Soc. Lond. A. 329:105-119.
24. Kaatz, P., and D. P. Shelton. 1999. Two-photon fluorescence cross-section measurements calibrated with hyper-Rayleigh scattering. J. Opt. Soc. Am. B. 16:998-1006.
25. Schönle, A. 2006. ImSpector Image Acquisition and Analysis Software, v0.1. http://www.imspector.de.
26. Clendenon, J. L., C. L. Phillips, R. M. Sandoval, S. Fang, and K. W. Dunn. 2002. Voxx: a PC-based, near real-time volume rendering system for biological microscopy. Am. J. Physiol. Cell Physiol. 282:C213-C218.
27. Xu, C., W. Zipfel, J. B. Shear, R. M. Williams, and W. W. Webb. 1996. Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy. Proc. Natl. Acad. Sci. USA. 93:10763-10768.
28. Blab, G. A., O. H. M. Lommerse, L. Cognet, G. S. Harms, and T. Schmidt. 2001. Two-photon excitation action cross-sections of the autofluorescent proteins. Chem. Phys. Lett. 350:71-77.
29. Callis, P. R. 1997. Two-photon-induced fluorescence. Annu. Rev. Phys. Chem. 48:271-297.
30. Macak, P., Y. Luo, P. Norman, and H. Agren. 2000. Electronic and vibronic contributions to two-photon absorption of molecules with multi-branched structures. J. Chem. Phys. 113:7055-7061.
31. Wiedenmann, J., A. Schenk, C. Röcker, A. Girod, K. D. Spindler, and G. U. Nienhaus. 2002. A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria). Proc. Natl. Acad. Sci. USA. 99:11646-11651.
32. Nienhaus, K., B. Vallone, F. Renzi, J. Wiedenmann, and G. U. Nienhaus. 2003. Crystallization and preliminary x-ray diffraction analysis of the red fluorescent protein eqFP611. Acta Crystallogr. D Biol. Crystallogr. 59:1253-1255.
33. Schenk, A., S. Ivanchenko, C. Röcker, J. Wiedenmann, and G. U. Nienhaus. 2004. Photodynamics of red fluorescent proteins studied by fluorescence correlation spectroscopy. Biophys. J. 86:384-394.
34. Hopt, A., and E. Neher. 2001. Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophys. J. 80:2029-2036.
35. Zipfel, W. R., R. M. Williams, and W. W. Webb. 2003. Nonlinear magic: multiphoton microscopy in the biosciences. Nat. Biotechnol. 21:1369-1377.
36. Staudt, T., M. C. Lang, R. Medda, J. Engelhardt, and S. W. Hell. 2006. 2,2′-Thiodiethanol: a new water soluble mounting medium for high resolution optical microscopy. Microsc. Res. Tech. doi 10.1002/jemt.20396.
37. Schrader, M., K. Bahlmann, G. Giese, and S. W. Hell. 1998. 4Pi-confocal imaging in fixed biological specimens. Biophys. J. 75:1659-1668.
38. Baddeley, D., C. Carl, and C. Cremer. 2006. 4Pi microscopy deconvolution with a variable point-spread function. Appl. Opt. 45:7056-7064.