Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM)

Nature Protocols

Volumn 2, Issue 5, 2007, Pages 1173-1179

  Allen, T D ^a   Rutherford, S A ^a   Murray, S ^a   Sanderson, H S ^b   Gardiner, F ^a   Kiseleva, E ^c   Goldberg, M W ^d   Drummond, S P ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

^c INSTITUTE OF CYTOLOGY AND GENETICS   (Russian Federation)

^d DURHAM UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL ACTIVITY; CELL FREE SYSTEM; CELL ISOLATION; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CHROMATIN; CONTROLLED STUDY; DNA REPLICATION; EGG; EGG LAYING; FEMALE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; MALE; NONHUMAN; NUCLEAR PORE COMPLEX; PRIORITY JOURNAL; QUALITY CONTROL; SCANNING ELECTRON MICROSCOPY; SPERM; STRUCTURE ANALYSIS; TEMPERATURE DEPENDENCE; WESTERN BLOTTING; XENOPUS LAEVIS;

ANIMALS; BLOTTING, WESTERN; CELL EXTRACTS; CELL NUCLEUS; CELL-FREE SYSTEM; CHROMATIN; FEMALE; MALE; MICROSCOPY, ELECTRON, SCANNING; OVUM; SPERMATOZOA; XENOPUS LAEVIS;

ANURA; EUKARYOTA; NES; XENOPUS LAEVIS;

EID: 34250206943     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2007.138     Document Type: Article

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