Cloning and characterization of glycogen-debranching enzyme from hyperthermophilic archaeon Sulfolobus shibatae

Journal of Microbiology and Biotechnology

Volumn 17, Issue 5, 2007, Pages 792-799

  Kim, Van Trinth Thi ^a   Ryu, Soo In ^a   Lee, Kyung Ju ^a   Kim, Eun Ju ^a   Lee, Soo Bok ^a  

^a Yonsei University   (South Korea)

Author keywords

1,4 glucanotransferase; Amylo 1,6 glucosidase; Glycogen debranching enzyme; Hyperthermophilic enzyme; Sulfolobus shibatae

Indexed keywords

6 O ALPHA MALTOSYL BETA CYCLODEXTRIN; ALCOHOL; ALPHA 1,4 GLUCANOTRANSFERASE; ALUMINUM; AMYLO 1,6 GLUCOSIDASE; AMYLOPECTIN; AMYLOSE; BARIUM ION; BETA CYCLODEXTRIN; CALCIUM ION; COBALT; COPPER ION; DEXTRIN; DIMETHYL SULFOXIDE; EDETIC ACID; GLYCOGEN; GLYCOGEN DEBRANCHING ENZYME; IRON; MAGNESIUM ION; MALTOSE; MALTOTRIOSE; MANGANESE; METHANOL; NICKEL; OLIGOSACCHARIDE; PULLULAN; RECOMBINANT ENZYME; STARCH; TRANSFERASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CARBOHYDRATE METABOLISM; CATALYSIS; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; HIGH TEMPERATURE; HYDROLYSIS; HYPERTHERMOPHILIC ARCHAEON; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SULFOLOBUS; SULFOLOBUS SHIBATAE; TEMPERATURE MEASUREMENT; THERMOSTABILITY;

AMYLOPECTIN; AMYLOSE; BETA-CYCLODEXTRINS; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GLUCANS; GLYCOGEN; GLYCOGEN DEBRANCHING ENZYME SYSTEM; HYDROGEN-ION CONCENTRATION; MALTOSE; OLIGOSACCHARIDES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; SULFOLOBUS; TEMPERATURE;

ARCHAEA; ESCHERICHIA COLI; SULFOLOBUS SHIBATAE;

EID: 34250184680     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Altschul, S. F., T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
2. Bae, S. S., Y. J. Kim, S. H. Yang, J. K. Lim, J. H. Jeon, H. S. Lee, S. G. Kang, S.-J. Kim, and J.-H. Lee. 2006. Thermococcus onnurineus sp. nov., a hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent area at the PACMANUS field. J. Microbiol. Biotechnol. 16: 1826-1831.
3. Bates, B. J., G. M. Heaton, C. Taylor, J. C. Kemohan, and P. Cohen. 1975. Debranching enzyme from rabbit skeletal muscle: Evidence for the location of two active centers on a single polypeptide chain. FEBS Lett. 58: 181-185.
4. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
5. Braun, C., T. Lindhorst, N. B. Madsen, and S. G. Withers. 1996 Identification of Asp 549 as the catalytic nucleophile of glycogen-debranching enzyme via trapping of the glycosyl-enzyme intermediate. Biochemistry 35: 5458-5463.
6. Dubnau, D. and R. D. Abenson. 1971. Fate of transforming DNA following uptake by competent Bacillus subtilis. J. Mol. Biol. 56: 209-221.
7. Duggleby, R. G. and D. R. Leonard. 1992. DNRPEASY, a data analysis computer program.
8. Fang, T. Y., W.-C. Tseng, C.-J. Yu, and T.-Y. Shih. 2005. Characterization of the thermophilic isoamylase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. J. Mol. Catal. B Enzym. 33: 99-107.
9. Fang, T. Y., X. G. Hung, T. Y. Shih, and W. C. Tseng. 2004. Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092. Extremophiles 8: 335-343.
10. Gillard, B. K. and T. E. Nelson. 1977. Amylo-1,6-glucosidase/ 4-α-glucanotransferase: Use of reversible substrate model inhibitors to study the binding and active sites of rabbit muscle debranching enzyme. Biochemistry 16: 3978-3987.
11. Kim, I. C., J. H. Cha, J. R. Kim, S. Y. Jang, B. C. Seo, T. K. Cheong, D. S. Lee, Y. D. Choi, and K. H. Park. 1992. Catalytic properties of the cloned amylase from Bacillus licheniformis. J. Biol. Chem. 267: 22108-22114.
12. Kim, M.-J., H.-E. Park, H.-K. Sung, T. H. Park, and J. Cha. 2005. Action mechanism of transfructosylation catalyzed by Microbacterium laevaniformans levansucrase. J. Microbiol. Biotechnol. 15: 99-104.
13. Kim, T. J., M. J. Kim, B. C. Kim, J. C. Kim, T. K. Cheong, J. W. Kim, and K. H. Park. 1999. Modes of action of acarbose hydrolysis and transglycosylation catalyzed by a thermostable maltogenic amylase, the gene for which was cloned from a Thermus strain. Appl. Environ. Microbiol. 65: 1644-1651.
14. Lee, E. Y. C. and W. J. Whelan. 1971. Glycogen and starch debranching enzyme, pp. 191-234. In P. D. Boyer (ed.), The Enzymes, 3rd Ed. Academic Press, New York, U.S.A.
15. Lee, E. Y. C., J. H. Carter, L. D. Nielsen, and E. H. Fischer. 1970. Purification and properties of yeast amylo-1,6-glucosidase-oligo-1,4-1,4-glucantransferase. Biochemistry 9: 2347-2355.
16. Liu, W, M. L. De Castro, J. Takrama, P. T. Bilous, T. Vinayagamoorthy, N. B. Madsen, and R. C. Bleackley. 1993. Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen-debranching enzyme. Arch. Biochem. Biophys. 306: 232-239.
17. Maruta, K., H. Mitsuzumi, T. Nakada, M. Kubota, H. Chaen, S. Fukuda, T. Sugimoto, and M. Kurimoto. 1996. Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius. Biochim. Biophys. Acta 1291: 177-181.
18. Maruta, K., M. Kubota, S. Fukuda, and M. Kurimoto. 2000. Cloning and nucleotide sequence of a gene encoding a glycogen-debranching enzyme in the trehalose operon from Arthrobacter sp. Q36. Biochim. Biophys. Acta 1476: 377-381.
19. Nakayama, A., K. Yamamoto, and S. Tabata. 2000. High expression of glycogen-debranching enzyme in Escherichia coli and its competent purification method. Protein Expr. Purif. 19: 298-303.
20. Nakayama, A., K. Yamamoto, and S. Tabata. 2001. Identification of the catalytic residues of bifunctional glycogen-debranching enzyme. J. Biol. Chem. 276: 28824-28828.
21. Paik, S.-K., H.-S. Yun, H. Iwahashi, K. Obuchi, and I. Jin. 2005. Effect of trehalose on stabilization of cellular components and critical targets against heat shock in Saccharomyces cerevisiae KNU5377. J. Microbiol. Biotechnol. 15: 965-970.
22. Ryu, S-I., C.-S. Park, S. Cha, E.-J. Woo, and S.-B. Lee. 2005. A novel trehalose-synthesizing glycosyltransferase from Pyrococcus horikoshii: Molecular cloning and characterization. Biochem. Biophys. Res. Commun. 329: 429-436.
23. Sambrook, J. and D. W. Russell. 2001. Molecular Cloning: A Laboratory Manual, 3rd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, U.S.A.
24. Taylor, C., A. S. Cox, J. C. Kernohan, and P. Cohen. 1975. Debranching enzyme from rabbit skeletal muscle: Purification, properties and physiological role. Eur. J. Biochem. 51: 105-115.
25. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
26. Urlaub, H. and G. Woeber. 1975. Identification of isoamylase, a glycogen-debranching enzyme, from Bacillus amyloliquefaciens. FEBS Lett. 57: 1-4.
27. Van der Maarel, M. J., B. Van der Veen, J. C. Uitdehaag, H. Leemhuis, and L. Dijkhuizen. 2002. Properties and applications of starch-converting enzymes of the α-amylase family. J. Biotechnol. 94: 137-155.
28. Watanabe, K., Y. Hata, H. Kizaki, Y. Katsube, and Y. Suzuki. 1997. The refined crystal structure of Bacillus cereus oligo-1,6-glucosidase at 2.0 Å resolution: Structural characterization of proline-substitution sites for protein thermostabilization. J. Mol. Biol. 269: 142-153.
29. Yang, B. Z., J. H. Ding, J. J. Enghild, Y. Bao, and Y.-T. Chen. 1992. Molecular cloning human muscle glycogen-debranching enzyme. J. Biol. Chem. 267: 9294-9299.
30. Yang, H., M. Y. Liu, and T. Romeo. 1996. Coordinate genetic regulation of glycogen catabolism and biosynthesis in Escherichia coli via the CsrA gene product. J. Bacteriol. 178: 1012-1017.