Structural and functional basis of amino acid specificity in the invertebrate cotransporter KAAT1

Journal of Physiology

Volumn 581, Issue 3, 2007, Pages 899-913

  Miszner, Andreea ^a   Peres, Antonio ^a   Castagna, Michela ^b   Bettè, Sara ^b   Giovannardi, Stefano ^a   Cherubino, Francesca ^a   Bossi, Elena ^a  

^a UNIVERSITY OF INSUBRIA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID TRANSPORTER; LEUCINE; PROLINE; PROTEIN CAATCH1; PROTEIN KAAT1; THREONINE; TRACER; UNCLASSIFIED DRUG; CAATCH1 PROTEIN; COTRANSPORTER; KAAT1 COTRANSPORTER PROTEIN; MEMBRANE PROTEIN; POTASSIUM ION; SODIUM ION;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT; ARTICLE; CALCIUM TRANSPORT; ELECTROPHYSIOLOGY; ENZYME SPECIFICITY; MANDUCA SEXTA; NONHUMAN; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STRUCTURE; REVERTANT; SODIUM TRANSPORT; AMINO ACID ANALYSIS; ANIMAL CELL; ANIMAL TISSUE; BINDING AFFINITY; BINDING SITE; COMPLEX FORMATION; CONTROLLED STUDY; FEMALE; MOLECULAR DYNAMICS; OOCYTE; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; RESIDUE ANALYSIS; STRUCTURE ANALYSIS; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; AMINO ACIDS; ANIMALS; BINDING, COMPETITIVE; CARRIER PROTEINS; COMPUTER SIMULATION; FEMALE; INSECT PROTEINS; KINETICS; LEUCINE; MANDUCA; MEMBRANE POTENTIALS; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; POTASSIUM; PROLINE; PROTEIN BINDING; PROTEIN CONFORMATION; SODIUM; THREONINE; XENOPUS LAEVIS;

EID: 34250029397     PISSN: 00223751     EISSN: 14697793     Source Type: Journal    
DOI: 10.1113/jphysiol.2007.132555     Document Type: Article

References (25)

1. Almers W & McCleskey EW (1984). Non-selective conductance in calcium channels of frog muscle: Calcium selectivity in a single-file pore. J Physiol 353, 585-608.
2. + symporters (NSS) aids in the useof the LeuT structure to probe NSS structure and function. Mol Pharmacol 70, 1630-1642.
3. Böhmer C, Bröer A, Munzinger M, Kowalczuk S, Rasko JEJ, Lang F & Bröer S (2005). Characterization of mouse amino acid transporter B0AT1 (slc6a19). Biochem J 389, 745-751.
4. Bossi E, Centinaio E, Castagna M, Giovannardi S, Vincenti S, Sacchi VF & Peres A (1999). Ion binding and permeation through the lepidopteran amino acid transporter KAAT1 expressed in Xenopus oocytes. J Physiol 515, 729-742.
5. Bossi E, Fabbrini MS & Ceriotti A (2007). Exogenous protein expression in Xenopus laevis oocyte: Basic procedure. In In Vitro Transcription and Translation Protocols, ed. Grandi G, pp. 107-131. Humana Press, Totowa, NJ, USA.
6. Boudko DY, Kohn AB, Meleshkevitch EA, Dasher MK, Seron TJ, Stevens BR & Harvey WR (2005). Ancestry and progeny of nutrient amino acid transporters. Proc Natl Acad Sci U S A 102, 1360-1365.
7. Castagna M, Shayakul C, Trotti D, Sacchi VF, Harvey WR & Hediger MA (1997). Molecular characteristics of mammalian and insect amino acid transporters: Implications for amino acid homeostasis. J Exp Biol 200, 269-286.
8. Castagna M, Shayakul C, Trotti D, Sacchi VF, Harvey WR & Hediger MA (1998). Cloning and characterization of a potassium-coupled amino acid transporter. Proc Natl Acad Sci U S A 95, 5395-5400.
9. Dow JAT & Peacock JM (1989). Microelectrode evidence for the electrical isolation of goblet cell cavities in Manduca sexta middle midgut. J Exp Biol 143, 101-114.
10. +, is alkaline pH-dependent, and is Cl -independent. J Biol Chem 275, 24518-24526.
11. Forlani G, Bossi E, Ghirardelli R, Giovannardi S, Binda F, Bonadiman L, Ielmini L & Peres A (2001). K448E mutation in the external loop 5 of rGAT1 transporter induces pH sensitivity and altered substrates interactions. J Physiol 536, 479-494.
12. i-2). J Gen Physiol 112, 1-18.
13. Hanozet GM, Sacchi VF, Nedergaard S, Bonfanti P, Magagnin S & Giordana B (1992). The K+ -driven amino acid cotransporter of the larval midgut of lepidoptera: Is Na+ an alternate substrate?J Exp Biol 162, 281-294.
14. Harvey WR, Wood JL, Quatrale RP & Jungreis AM (1975). Cation distributions across the larval and pupal midgut of the lepidopteran, Hyalophora cecropia, in vivo. J Exp Biol 63, 321-330.
15. Hennigan BB, Wolfersberger MG, Parthasarathy R & Harvey WR (1993). Cation-dependent leucine, alanine, and phenylalanine uptake at pH 10 in brush-border membrane vesicles from larval Manduca sexta midgut. Biochim Biophys Acta 1148, 209-215.
16. Hess P & Tsien RW (1984). Mechanism of ion permeation through calcium channels. Nature 309, 453-456.
17. Läuger P (1991). Electrogenic Ion Pumps. Sinauer Associates, Sunderland, MA, USA.
18. Mari SA, Soragna A, Castagna M, Bossi E, Peres A & Sacchi VF (2004). Aspartate 338 contributes to the cationic specificity and to the driver-amino acid coupling in the insect cotransporter KAAT1. Cell Mol Life Sci 61, 243-256.
19. +/glucose cotransporter: II. A transport model under nonrapid equilibrium conditions. J Membr Biol 125, 63-79.
20. Peres A & Bossi E (2000). Effects of pH on the uncoupled, coupled and presteady-state currents at the amino acid cotransporter KAAT1 expressed in Xenopus oocytes. J Physiol 525, 83-89.
21. Quick M & Stevens BR (2001). Amino acid transporter CAATCH1 is also an amino acid-gated cation channel. J Biol Chem 276, 33413.
22. +/oligopeptide cotransporter, hPEPT1. J Physiol 574, 149-166.
23. Soragna A, Mari S, Peres A, Pisani R, Castagna M, Sacchi VF & Bossi E (2004). Structural domains involved in substrate selectivity in two neutral amino acid transporters. Am J Physiol Cell Physiol 287, C754-C761.
24. Vincenti S, Castagna M, Peres A & Sacchi VF (2000). Substrate selectivity and pH dependence of KAAT1 expressed in Xenopus laevis oocytes. J Membr Biol 174, 213-224.
25. --dependent neurotransmitter transporters. Nature 437, 215-223.