Identification of the neuropeptide content of individual rat neurohypophysial terminals

Journal of Neuroscience Methods

Volumn 163, Issue 2, 2007, Pages 226-234

  Custer, Edward E ^a   Ortiz Miranda, Sonia ^a   Knott, Thomas K ^a   Rawson, Randi ^a   Elvey, Christian ^a   Lee, Ryan H ^a   Lemos, José R ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

AVP; ELISA; Immunocytochemistry; OT; Posterior pituitary

Indexed keywords

ARGIPRESSIN; NEUROPEPTIDE; OXYTOCIN;

ANIMAL TISSUE; ARTICLE; BRAIN ELECTROPHYSIOLOGY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOCYTOCHEMISTRY; MALE; NEUROHYPOPHYSIS; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; RAT;

ANIMALS; ARGININE VASOPRESSIN; BIOLOGICAL ASSAY; ENZYME-LINKED IMMUNOSORBENT ASSAY; IMMUNOHISTOCHEMISTRY; MALE; NEURAL PATHWAYS; NEUROCHEMISTRY; NEUROPEPTIDES; OXYTOCIN; PARAVENTRICULAR HYPOTHALAMIC NUCLEUS; PITUITARY GLAND, POSTERIOR; PRESYNAPTIC TERMINALS; RATS; RATS, SPRAGUE-DAWLEY; SUPRAOPTIC NUCLEUS; SYNAPTIC TRANSMISSION;

EID: 34249887080     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.03.006     Document Type: Article

References (19)

1. Ben-Barak Y., Russell J.T., Whitnall M.H., Ozato K., and Gainer H. Neurophysin in the hypothalamo-neurohypophysial system. 1. Production and characterization of monoclonal antibodies. J Neurosci 5 (1985) 81-97
2. Bindler E., Labella F.S., and Sanwal M. Isolated nerve endings (neurosecretosomes) from the posterior pituitary. Partial separation of vasopressin and oxytocin and the isolation of microvesicles. J Cell Biol 34 1 (1967) 185-205
3. Cazalis M., Dayanithi G., and Nordmann J.J. Hormone release from isolated nerve endings of the rat neurohypophysis. J Physiol 390 (1987) 55-70
4. Knott T.K., Velazquez-Marrero C., and Lemos J.R. ATP elicits inward currents in isolated vasopressinergic neurohypophysial terminals via P2X2 and P2X3 receptors. Pflugers Arch 450 6 (2005) 381-389
5. Lemos J.R. Magnocellular neurons. Encyc Life Sci A176 (2001) 1-5 10.1038/npg.els.000176
6. Lemos J.R., and Nordmann J.J. Ionic channels and hormone release from peptidergic nerve terminals. J Exp Biol 24 (1986) 53-72
7. Lemos J.R., and Nowycky M.C. Two types of calcium channels coexist in peptide-releasing vertebrate nerve terminals. Neuron 2 (1989) 1419-1426
8. Mezey E., and Kiss J.Z. Coexpression of vasopressin and oxytocin in hypothalamic supraoptic neurons of lactating rats. Endocrinology 129 4 (1991) 1814-1820
9. 2+ influx. J Neuroendocrinol 15 (2003) 888-894
10. 2+ channels. J Neuroendocrinol 17 9 (2005) 583-590
11. OuYang W., Wang G., and Hemmings Jr. H.C. Distinct rat neurohypophysial nerve terminal populations identified by size, electrophysiological properties and neuropeptide content. Brain Res 1024 (2004) 203-211
12. Schoenfeld T.A., and Knott T.K. Evidence for the disproportionate mapping of olfactory airspace onto the main olfactory bulb of the hamster. J Comp Neurol 476 2 (2004) 186-201
13. Toescu E.C., and Morris J.F. Morphometric analysis of nerve endings isolated from bovine and rat neurohypophysis. J Anat 3 (1990) 1-17
14. 2X2 purinoceptor. J Physiol 511 (1998) 89-103
15. 2+ channels in vasopressin secretion from neurohypophysial terminals of the rat. J Physiol 502 (1997) 351-363
16. 2+ current in neurohypophysial terminals preferentially regulates oxytocin secretion. J Neurosci 19 (1999) 9235-9241
17. Wang X., Treistman S.N., and Lemos J.R. Two types of high threshold calcium currents inhibited by ω-conotoxin in nerve terminals of rat neurohypophysis. J Physiol 445 (1992) 181-199
18. Wang X., Treistman S.N., Wilson A., Nordmann J.J., and Lemos J.R. Calcium channels and peptide release from neurosecretory terminals. NIPS 8 (1993) 64-68
19. Wang X.M., Treistman S.N., and Lemos J.R. Direct identification of individual vasopressin-containing nerve terminals of the rat neurohypophysis after 'whole-cell' patch-clamp recordings. Neurosci Lett 11 1 (1991) 125-128 124