Production of antibodies for the quantitative detection of the anabolically active androgens 17β-boldenone and methylboldenone

Analytical Letters

Volumn 40, Issue 7, 2007, Pages 1461-1472

  Kramer, K ^a   Hubauer, A ^a   Lausterer, R ^a   Salvador, J P ^b   Marco, M P ^b  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^b DEPARTMENT OF ENVIRONMENTAL CHEMISTRY   (Spain)

Author keywords

17 boldenone; Enzyme linked immunosorbent assay; Methylboldenone; Monoclonal antibody; Polyclonal antibody

Indexed keywords

EID: 34249864485     PISSN: 00032719     EISSN: 1532236X     Source Type: Journal    
DOI: 10.1080/00032710701327112     Document Type: Article

References (14)

1. Beiser, S.M. and Erlanger, B.F. 1967. Estimation of steroid hormones by an immunochemical technique. Nature, 214: 1044-1045.
2. Buiarelli, F., Cartoni, G.P., Amendola, L., and Botre, F. 2005. Detection of boldenone and its major metabolites by liquid chromatography - tandem mass spectrometry in urine samples. Anal. Chim. Acta, 552: 116-126.
3. De Brabander, H., Batjoens, P., Courtheyn, D., Vercammen, J., and De Wasch, K. 1996. Comparison of the possibilities of gas chromatography-mass spectrometry and tandem mass spectrometry systems for the analysis of anabolics in biological material. J. Chromatogr. A, 750: 105-114.
4. De Brabander, H.F., Poelmans, S., Schilt, R., Stephany, R.W., Le Bizec, B., Draiscik, R., Sterk, S.S., van Ginkel, L.A., Courtheyn, D., Van Hoof, N., Macri, A., and Wasch, K.De. 2004. Presence and metabolism of the anabolic steroid boldenone in various animal species: A review. Food Addit. Contam., 21: 515-525.
5. Draisci, R., Palleschi, L., Ferretti, E., Lucentini, L., and Delli Quadri, F. 2003. Confirmatory analysis of 17[beta]-boldenone, 17[alpha]-boldenone and androsta-1,4-diene-3,17-dione in bovine urine by liquid chromatography-tandem mass spectrometry. J. Chromatogr B, 789: 219-226.
6. Erlanger, B.F. 1967. Estimation of steroid hormiones by an immunochemical technique. Nature, 214: 1044.
7. Estévez, M.-C., Galve, R., Sánchez-Baeza, F., and Marco, M.-P. 2005. A direct competitive enzyme-linked immunosorbent assay (ELISA) for the determination of the highly polar short chain sulfophenylcarboxylates (SPC). Anal. Chem., 77: 5283-5293.
8. Goding, J.W. 1983. Monoclonal Antibodies: Principles and Practice, 1st edn.; Academic Press: London.
9. Hungerford, N.L., Sortais, B., Smart, C.G., McKinney, A.R., Ridley, D.D., Stenhouse, AM., and Al-Dujaili, E.A.S. 2006. Development and application of a simple radioimmunoassay for urinary aldosterone. Clin. Chim. Acta, 364: 172-179.
10. Rodriguez-Mozaz, S., Marco, M.-P., Lopez de Alda, M.J., and Barceló, D. 2004. Biosensors for environmental monitoring of endocrine disruptors: A review article. Anal. Bioanal. Chem., 378: 588-598.
11. Schaenzer, W., Delahaut, P., Geyer, H., Machnik, M., and Horning, S. 1996. Long-term detection and identification of metandienone and stanozolol abuse in athletes by gas chromatography-high-resolution mass spectrometry. J. Chromatogr. B, 687: 93-108.
12. Stähli, C., Staehlin, T., Miggiano, V., Schmidt, J., and Hbing, P. 1980. High frequencies of antigen-specific hybridomas: Dependence on immunization parameters and prediction by spleen cell analysis. J. Immunol. Meth., 32: 297-304.
13. Suann, C.J., Munn, K.J., Sillence, M.N., and McLeod, M.D. 2005. Analysis of anabolic steroids in the horse: Development of a generic ELISA for the screening of 17 β-alkyl anabolic steroid metabolites. J. Steroid Biochem Mol Biol, 96: 317-334.
14. Van Poucke, C. and Van Peteghem, C. 2002. Development and validation of a multianalyte method for the detection of anabolic steroids in bovine urine with liquid chromatography-tandem mass spectrometry. J. Chromat. B, 772: 211-217.