Development and validation of a simple and direct ELISA method for the determination of conjugated (glucuronide) and non-conjugated testosterone excretion in urine

Clinica Chimica Acta

Volumn 364, Issue 1-2, 2006, Pages 172-179

  Al Dujaili, Emad A S ^a  

^a QUEEN MARGARET UNIVERSITY   (United Kingdom)

Author keywords

Assay; Determination; ELISA; Testosterone; Urine

Indexed keywords

ANTIBODY; GLUCURONIDE; HORSERADISH PEROXIDASE; IMMUNOGLOBULIN G; STEROID; TESTOSTERONE;

ACCURACY; ADULT; ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HYDROLYSIS; INTERMETHOD COMPARISON; MALE; PARALLEL DESIGN; PRIORITY JOURNAL; SENSITIVITY AND SPECIFICITY; SEX DIFFERENCE; SOLVENT EXTRACTION; URINALYSIS; URINARY EXCRETION; VALIDATION PROCESS;

ADULT; ANIMALS; ANTIBODY SPECIFICITY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GLUCURONIDES; HUMANS; IMMUNE SERA; MALE; MIDDLE AGED; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; TESTOSTERONE;

ARMORACIA RUSTICANA; EQUUS ASINUS; OVIS ARIES;

EID: 31044456138     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cccn.2005.06.019     Document Type: Article

References (43)

1. H.G. Burger Androgen production in women Fertil Steril 77 Suppl. 4 2002 S3 S5
2. E. Venturelli, A. Cavalleri, and G. Secreto Methods for urinary testosterone analysis J Chromatogr 671 1995 363 380
3. N.C. Onland-Moret, R. Kaaks, and P.A. van Noord Urinary endogenous sex hormone levels and the risk of postmenopausal breast cancer Br J Cancer 88 2003 1394 1399
4. V. Poor, S. Juricskay, and E. Telegdy Urinary steroids in men with male-pattern alopecia J Biochem Biophys Methods 53 2002 123 130
5. M.V. Hall, H.L. Leathard, and J. Coley Urinary hormone levels during the natural menstrual cycle: the effect of age J Endocrinol 170 2001 157 164
6. S. Juricskay, and E. Telegdy Urinary steroids in women with androgenic alopecia Clin Biochem 33 2000 97 101
7. C. Gambelunghe, R. Rossi, and M. Sommavilla Effects of chrysin on urinary testosterone levels in human males J Med Food 6 2003 387 390
8. K. van Uytfanghe, D. Stock, and J.M. Kaufman Evaluation of a candidate reference measurement procedure for serum free testosterone based on ultrafiltration and isotope dilution-gas chromatography-mass spectrometry Clin Chem 50 2004 2101 2110
9. M.H. Choi, J.N. Kim, and B.C. Chung Rapid HPLC-electrospray tandem mass spectrometric assay for urinary testosterone and dihydrotestosterone glucuronides from patients with benign prostate hyperplasia Clin Chem 49 2003 322 325
10. A.T. Kicman, S.B. Coutts, and D.A. Cowan Adrenal and gonadal contributions to urinary excretion and plasma concentration of epitestosterone in men-effect of adrenal stimulation and implications for detection of testosterone abuse Clin Endocrinol 50 1999 661 668
11. P. Vierhapper, P. Nowotny, and W. Waldhausl Determination of testosterone production rates in men and women using stable isotope/dilution and mass spectrometry J Clin Endocrinol Metab 86 1997 1492 1496
12. L. Dehennin On the origin of physiologically high ratios of urinary testosterone to epitestosterone: consequences for reliable detection of testosterone administration by male athletes J Endocrinol 142 1994 353 360
13. S.A. Wudy, J. Homoki, and W.M. Teller Profiling plasma androgens: a SIM GC/MS method suitable for routine clinical application S. Gorog Proc 4th Symp Anal Steroids 1990 235 239
14. B.K. Yap, R. Kazlauskas, and K. Elghazi Profiling of urinary testosterone and luteinizing hormone in exercise-stressed male athletes, using gas chromatography-mass spectrometry and enzyme immunoassay techniques J Chromatogr B Biomed Appl 687 1996 117 125
15. R. Gonzalo-Lumbreras, D. Pimentel-trapero, and R. Izquierdo-Hornilos Development and method validation for testosterone and epitestosterone in human urine samples by liquid chromatography applications J Chromatogr Sci 41 2003 261 265
16. A. Turkes, A.O. Turkes, and B.G. Joyce A sensitive solid phase enzyme immunoassay for testosterone in plasma and saliva Steroids 33 1979 347 359
17. C. Hanquez, P. Urios, and B. Desfosses Enzyme-linked immunosorbant assay (ELISA) for steroid hormones with polyclonal and monoclonal antibodies: an assay for urinary aldosterone Clin Chim Acta 164 1987 71 82
18. G.E. Rukavishnikova, B.B. Dzantiev, and A.L. Liozner Enzyme immunoassay for testosterone with the use of protein A from Staphylococcus aureus S. Gorog Proc 4th Symp Anal Steroids 1990 103 107
19. M. Rassaie, L.G. Kumari, and P.K. Pandey A highly specific heterologus enzyme-linked immunosorbant assay for measuring testosterone in plasma using antibody-coated immunoassay plates or polypropylene tubes Steroids 57 1992 288 294
20. R.F. Walker, D.W. Wilson, G.F. Read, and D. Riad-Fahmy Assessment of testicular function by radioimmunoassay of testosterone in saliva Int J Androl 3 1980 105 120
21. M. Sowers, J.F. Randolph, and M. Crutchfield Urinary ovarian gonadotropin hormone levels in premenopausal women with low bone mass J Bone Miner Res 13 1998 1191 1202
22. V.A. Bricout, F. Wright, and M. Lagoguey Urinary profile of androgen metabolites in a population of sportswomen during the menstrual cycle Int J Sports Med 24 2003 197 202
23. M. Zitzmann, and E. Nieschlag Testosterone levels in healthy men and the relation to behavioural and physical characteristics: facts and constructs Eur J Endocrinol 144 2001 183 197
24. D.B. Gower Extraction, purification and estimation of the androgens and their derivatives H.L.J. Makin D.B. Gower D.N. Kirk Steroid analysis 1995 Blackie Academic & Professional Glasgow 268 368
25. C.P. Price The evolution of immunoassay as seen through the journal clinical chemistry Clin Chem 44 1998 2071 2074
26. J.H. Yeung, J.K. Wong, and B.K. Park Development of a monoclonal antibody to 6-beta-hydroxycotisol and its application in an enzyme-linked immunosorbant assay for 6-beta-hydroxy cortisol in urine J Pharmacol Toxicol Methods 38 1997 71 79
27. J.G. Lewis, and P.A. Elder An enzyme-linked immunosorbant assay (ELISA) for plasma cortisol J Steroid Biochem 22 1985 673 676
28. V. Ferchaud, P. Courcoux, and B. Le Bizec Enzymatic hydrolysis of conjugated steroid metabolites: search for optimum conditions using response surface methodology Analyst 125 2000 2255 2259
29. H. Shibasaki, C. Tanabe, and T. Furuta Hydrolysis of conjugated steroids by the combined use of β-glucuronidase preparations from Helix pomatia and ampularia: determination of urinary cortisol and is metabolites Steroids 66 2001 795 801
30. G.E. Abraham Solid-phase radioimmunoassay for oestradiol-17β J Clin Endocrinol Metab 29 1969 866 870
31. E.A.S. Al-Dujaili, M. Denyer, C. Milne, R. Philo, J. Pritchard, and A. Allen Serozyme: a manual non-isotopic immunoassay system. Immunometric assays; overview and new developments Proceedings of NEQAS meeting, Cardiff 1988 1 8
32. E.A.S. Al-Dujaili, and C.R.W. Edwards The development and application of a simple radioimmunoassay for urinary aldosterone Clin Chim Acta 116 1981 277 287
33. G.E. Abraham Radioimmunoassay of steroids in biological materials Acta Endocrinol 183 1974 7 42
34. S.M. Atherden, J.E.T. Corrie, and E.A.S. Al-Dujaili Development and application of a direct radioimmunoassay for aldosterone in saliva Steroids 46 1985 845 855
35. J. Taieb, B. Mathian, and F. Millot Testosterone measured by 10 immunoassays and by isotop-dilution gas chromatography-mass spectrometry in sera from 116 men, women and children Clin Chem 49 2003 1381 1395
36. W. Bartsch, and K.D. Voigt Hydrolysis of steroid conjugates Methods of enzymatic analysis 3rd edition 1985 Verlag Chemie Weinheim 161
37. H.U. Bergmeyer, M. Grassel, and H.E. Walter H.U. Bergmeyer Methods of enzymatic analysis vol. 2 1983 VCH Weinheim 206 207
38. C.H.L. Shackleton Analysis of steroids H. Jaegar Gas capillary chromatography in clinical medicine and pharmacology 1985 Marcel Dekker New York 153 243
39. J.F. Dunn, B.C. Nisula, and D. Rodbard Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma J Clin Endocrinol Metab 53 1981 58 68
40. A. Vermeulin, J.M. Kaufman, and V.A. Giaguli Influence of some biological indexes on sex hormone binding globulin and androgen levels in ageing or obese males J Clin Endocrinol Metab 81 1996 1821 1826
41. R. Mitamura, K. Yano, N. Suzuki, Y. Ito, Y. Makita, and A. Okuno Diurnal rhythms of luteinizing hormone, follicle-stimulating hormone and testosterone secretion before the onset of male puberty J Clin Endocrinol Metab 84 1999 29 37
42. E. Venturelli, A. Manzari, A. Cavalleri, M. Benzo, G. Secreto, and E. Marubini Urinary testosterone measurement by gas chromatography after solid-phase extraction and high-performance liquid chromatography J Chromatogr 582 1992 7 12
43. H.W. Hagedorn, and R. Schulz Identification and verification of the anabolic steroid boldenone in equine blood and urine by HPLC/ELISA Biomed Chromatogr 8 1994 63 68