Detection of resistance to rifampicin and decreased susceptibility to penicillin in Neisseria meningitidis by real-time multiplex polymerase chain reaction assay

Diagnostic Microbiology and Infectious Disease

Volumn 58, Issue 2, 2007, Pages 241-244

  Stefanelli, Paola ^a   Neri, Arianna ^a   Carattoli, Alessandra ^a   Mastrantonio, Paola ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

Neisseria meningitidis; Penicillin; Real time PCR; Rifampicin

Indexed keywords

PENICILLIN G; RIFAMPICIN;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CEREBROSPINAL FLUID; FLUORESCENCE RESONANCE ENERGY TRANSFER; NEISSERIA MENINGITIDIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PENICILLIN RESISTANCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RPOB GENE;

ANTI-BACTERIAL AGENTS; CEREBROSPINAL FLUID; DNA PRIMERS; DRUG RESISTANCE, MULTIPLE, BACTERIAL; HUMANS; NEISSERIA MENINGITIDIS; PENICILLIN RESISTANCE; PENICILLINS; POLYMERASE CHAIN REACTION; RIFAMPIN; SENSITIVITY AND SPECIFICITY;

EID: 34249830142     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2006.11.022     Document Type: Article

References (13)

1. Antignac A., Kriz P., Tzanakaki G., Alonso J.M., and Taha M.K. Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin. J. Antimicrob. Chemother. 47 (2001) 285-296
2. Antignac A., Boneca I.G., Rouselle J.C., Namane A., Carlier J.P., Vazquez J.A., Fox A., Alonso J.M., and Taha M.K. Correlation between alterations of the penicillin-binding protein 2 and modifications of the peptidoglycan structure in Neisseria meningitidis with reduced susceptibility to penicillin G. J. Biol. Chem. 278 (2003) 31529-31535
3. Arreaza L., Salcedo C., Alcala B., Uria M.J., Abad R., Enriquez R., and Vazquez J.A. Sequencing of Neisseria meningitidis penA gene: the key to success in defining penicillin G breakpoints. Antimicrob. Agents Chemother. 48 (2004) 358-359
4. Carter P.E., Abadi J.R., Yakubu D.E., and Pennington T.H. Molecular characterization of rifampicin-resistant Neisseria meningitidis. Antimicrob. Agents Chemother. 38 (1994) 1256-1261
5. Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; Fifteenth Informational Supplement M-100-S15 Vol 25 N.1 (2005), Clinical Laboratory Standards Institute, Wayne, PA
6. Mastrantonio P., Stefanelli P., Fazio C., Sofia T., Neri A., La Rosa G., Marianelli C., Muscillo M., Caporali M.G., and Salmaso S. Serotype distribution, antibiotic susceptibility, and genetic relatedness of Neisseria meningitidis strains recently isolated in Italy. Clin. Infect. Dis. 36 (2003) 422-428
7. Nolte O. Rifampicin resistance in Neisseria meningitidis: evidence from a study of sibling strains, description of new mutations and notes on population genetics. J. Antimicrob. Chemother. 39 (1997) 747-755
8. Public Health Laboratory Service, Public Health Medicine Environmental Group, and Scottish Centre for Infection and Environmental Health. Guidelines for public health management of meningococcal disease in the UK. Commun. Dis. Public Health 5 (2002) 187-204
9. Richter S.S., Gordon K.A., Rhomberg P.R., Pfaller M.A., and Jones R.N. Neisseria meningitidis with decreased susceptibility to penicillin: report from the SENTRY antimicrobial surveillance program, North America, 1988-99. Diagn. Microbiol. Infect. Dis. 41 (2001) 83-88
10. Saez-Nieto J.A., Lujan R., Berron S., et al. Epidemiology and molecular basis of penicillin resistant Neisseria meningitidis in Spain: a 5-year history (1985-1989). Clin. Infect. Dis. 14 (1992) 394-402
11. Stefanelli P., Fazio C., La Rosa G., Marianelli C., Muscillo M., and Mastrantonio P. Rifampicin-resistant meningococci causing invasive disease: detection of point mutations in the rpoB gene and molecular characterization of the strains. J. Antimicrob. Chemother. 47 (2001) 219-222
12. Stefanelli P., Carattoli A., Neri A., Fazio C., and Mastrantonio P. Prediction of decreased susceptibility to penicillin of Neisseria meningitidis strains by real-time PCR. J. Clin. Microbiol. 41 (2003) 4666-4670
13. Stefanelli P., Carattoli A., Neri A., Sofia T., and Mastrantonio P. Emergence in Italy of a Neisseria meningitidis clone with decreased susceptibility to penicillin. Antimicrob. Agents Chemother. 48 (2004) 3103-3106