Use of fluorescent in situ hybridisation for the visualisation of Helicobacter pylori in real drinking water biofilms

Water Science and Technology

Volumn 55, Issue 8-9, 2007, Pages 387-393

  Bragança, Sofia M ^a   Azevedo, Nuno F ^a,b   Simöes, Lucia Chaves ^a   Keevil, C W ^b   Vieira, M J ^a  

^a UNIVERSITY OF MINHO   (Portugal)

^b UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Biofilms; Drinking water; Helicobacter pylori; Peptide nucleic acids; Rapid detection methods

Indexed keywords

BIOFILMS; FLUORESCENCE MICROSCOPY; MORPHOLOGY; PATHOGENS; POTABLE WATER; WATER ANALYSIS; WATER DISTRIBUTION SYSTEMS; WATER PIPING SYSTEMS;

HELICOBACTER PYLORI; PEPTIDE NUCLEIC ACIDS; RAPID DETECTION METHODS;

BACTERIA;

DRINKING WATER; PEPTIDE NUCLEIC ACID;

BACTERIA; BIOFILMS; FLUORESCENCE MICROSCOPY; MORPHOLOGY; PATHOGENS; POTABLE WATER; WATER ANALYSIS; WATER DISTRIBUTION SYSTEMS; WATER PIPING SYSTEMS;

BACTERIUM; BIOFILM; DRINKING WATER; FLUORESCENCE SPECTROSCOPY; HYBRIDIZATION; NUCLEIC ACID; PEPTIDE; VISUALIZATION;

BIOFILM; CONFERENCE PAPER; DIAGNOSTIC ACCURACY; EPIFLUORESCENCE MICROSCOPY; FLUOROSCOPY; HELICOBACTER PYLORI; HYBRIDIZATION; IN SITU HYBRIDIZATION; MICROORGANISM DETECTION; NONHUMAN; STAFF TRAINING; VALIDITY; WATER ANALYSIS; WATER SUPPLY;

BIOFILMS; HELICOBACTER PYLORI; IN SITU HYBRIDIZATION, FLUORESCENCE; PEPTIDE NUCLEIC ACIDS; WATER POLLUTANTS; WATER SUPPLY;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI;

EID: 34249650072     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2007.282     Document Type: Conference Paper

References (31)

1. Axon, A. (2006). Helicobacter pylori: what do we still need to know? J. Clin. Gastroenterol., 40, 15-19.
2. Azevedo, N.F., Pacheco, A.P., Vieira, M.J. and Keevil, C.W. (2004). Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water. Appl. Environ. Microbiol., 70, 490-493.
3. Azevedo, N.F., Pinto, A.R., Reis, N.M., Vieira, M.J. and Keevil, C.W. (2006). Shear stress, temperature, and inoculation concentration influence the adhesion of water-stressed Helicobacter pylori to stainless steel 304 and polypropylene. Appl. Environ. Microbiol., 72, 2936-2941.
4. Azevedo, N.F., Vieira, M.J. and Keevil, C.W. (2003a). Development of peptide nucleic acid probes to detect Helicobacter pylori in diverse species potable water biofilms. In Biofilm Communities: Order From Chaos? McBain, A., Allison, D., Brading, M., Rickard, A., Verran, J. and Walker, J. (eds), BioLine, Cardiff, pp. 105-112.
5. Azevedo, N.F., Vieira, M.J. and Keevil, C.W. (2003b). Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms. Wat. Sci. Tech., 47, 155-160.
6. Baschong, W., Suetterlin, R. and Laeng, R.H. (2001). Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J. Histochem. Cytochem., 49, 1565-1571.
7. Baudart, J., Olaizola, A., Coallier, J., Gauthier, V. and Laurent, P. (2005). Assessment of a new technique combining a viability test, whole-cell hybridization and laser-scanning cytometry for the direct counting of viable Enterobacteriaceae cells in drinking water. FEMS Microbiol. Lett., 243, 405-409.
8. Bellack, N.R., Koehoorn, M.W., Macnab, Y.C. and Morshed, M.G. (2006). A conceptual model of water's role as a reservoir in Helicobacter pylori transmission: a review of the evidence. Epidemiol. Infec., 134, 439-449.
9. Benson, J.A., Fode-Vaughan, K.A. and Collins, M.L.P. (2004). Detection of Helicobacter pylori in water by direct PCR. Lett. Appl. Microbiol., 39, 221-225.
10. Blaser, M.J. and Atherton, J.C. (2004). Helicobacter pylori persistence: biology and disease. J. Clin. Invest., 113, 321-333.
11. Bunn, J.E.G., MacKay, W.G., Thomas, J.E., Reid, D.C. and Weaver, L.T. (2002). Detection of Helicobacter pylori DNA in drinking water biofilms: implications for transmission in early life. Lett. Appl. Microbiol., 34, 450-454.
12. Conchello, J.A. and Lichtman, J.W. (2005). Optical sectioning microscopy. Nat. Meth., 2, 920-931.
13. Flanigan, D. and Rodgers, M. (2003). A method to detect viable Helicobacter pylori bacteria in groundwater. Acta Hydroch. Hydrob., 31, 45-48.
14. Hallam, N.B., West, J.R., Forster, C.F. and Simms, J. (2001). The potential for biofilm growth in water distribution systems. Wat. Res., 35, 4063-4071.
15. Keevil, C.W. (2003). Rapid detection of biofilms and adherent pathogens using scanning confocal laser microscopy and episcopic differential interference contrast microscopy. Wat. Sci. Tech., 47, 105-116.
16. Krumbiegel, P., Lehmann, I., Alfreider, A., Fritz, G.J., Boeckler, D., Rolle-Kampczyk, U., Richter, M., Jorks, S., Muller, L., Richter, M.W. and Herbarth, O. (2004). Helicobacter pylori determination in non-municipal drinking water and epidemiological findings. Isot. Environ. Health S., 40, 75-80.
17. Lehtola, M.J., Juhna, T., Miettinen, I.T., Vartiainen, T. and Martikainen, P.J. (2004). Formation of biofilms in drinking water distribution networks, a case study in two cities in Finland and Latvia. J. Ind. Microbiol. Biol., 31, 489-494.
18. Lehtola, M.J., Loades, C.J. and Keevil, C.W. (2005). Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari. J. Microbiol. Meth., 62, 211-219.
19. Lehtola, M.J., Torvinen, E., Miettinen, L.T. and Keevil, C.W. (2006). Fluorescence in situ hybridization using peptide nucleic acid probes for rapid detection of Mycobacterium avium subsp avium and Mycobacterium avium subsp paratuberculosis in potable-water biofilms. Appl. Environ. Microbiol., 72, 848-853.
20. Lu, Y.Z., Redlinger, T.E., Avitia, R., Galindo, A. and Goodman, K. (2002). Isolation and genotyping of Helicobacter pylori from untreated municipal wastewater. Appl. Environ. Microbiol., 68, 1436-1439.
21. Moreno, Y., Ferrus, M.A., Alonso, J.L., Jimenez, A. and Hernandez, J. (2003). Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water. Wat. Res., 37, 2251-2256.
22. Neumann, M. and Gabel, D. (2002). Simple method for reduction of autofluorescence in fluorescence microscopy. J. Histochem. Cytochem., 50, 437-439.
23. Nielsen, P.E., Egholm, M., Berg, R.H. and Buchardt, O. (1991). Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide. Science, 254, 1497-1500.
24. Park, S.R., Mackay, W.G. and Reid, D.C. (2001). Helicobacter sp. recovered from drinking water biofilm sampled from a water distribution system. Wat. Res., 35, 1624-1626.
25. Piqueres, P., Moreno, Y., Alonso, J.L. and Ferrus, M.A. (2006). A combination of direct viable count and fluorescent in situ hybridization for estimating Helicobacter pylori cell viability. Res. Microbiol., 157, 345-349.
26. Prescott, A.M. and Fricker, C.R. (1999). Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water. Mol. Cell. Probes, 13, 261-268.
27. Rolle-Kampczyk, U.E., Fritz, G.J., Diez, U., Lehmann, I., Richter, M. and Herbarth, O. (2004). Well water - one source of Helicobacter pylori colonization. Int. J. Hyg. Environ. Health, 207, 363-368.
28. Savichtcheva, O., Okayama, N., Ito, T. and Okabe, S. (2005). Application of a direct fluorescence-based live/dead staining combined with fluorescence in situ hybridization for assessment of survival rate of Bacteroides spp. in drinking water. Biotechnol. Bioeng., 92, 356-363.
29. Stender, H., Fiandaca, M., Hyldig-Nielsen, J.J. and Coull, J. (2002). PNA for rapid microbiology. J. Microbiol. Methods, 48, 1-17.
30. Watson, C.L., Owen, R.J., Said, B., Lai, S., Lee, J.V., Surman-Lee, S. and Nichols, G. (2004). Detection of Helicobacter pylori by PCR but not culture in water and biofilm samples from drinking water distribution systems in England. J. Appl. Microbiol., 97, 690-698.
31. Winiecka-Krusnell, J., Wreiber, K., Von Euler, A., Engstrand, L. and Linder, E. (2002). Free-living amoebae promote growth and survival of Helicobacter pylori. Scand. J. Infect. Dis., 34, 253-256.