PNA for rapid microbiology

Journal of Microbiological Methods

Volumn 48, Issue 1, 2002, Pages 1-17

  Stender, Henrik ^a   Fiandaca, Mark ^a   Hyldig Nielsen, Jens J ^a   Coull, James ^a  

^a Boston Probes Inc   (United States)

Author keywords

PNA; Rapid microbiology; rRNA sequence

Indexed keywords

DNA; PEPTIDE NUCLEIC ACID; RIBOSOME RNA;

BACTERIUM; BACTERIUM CULTURE; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; DNA PROBE; GENE AMPLIFICATION; GENE SEQUENCE; GENE TARGETING; LABORATORY TEST; MEMBRANE FILTER; METHODOLOGY; MICROBIOLOGICAL PARAMETERS; MICROBIOLOGY; MICROSCOPY; MYCOBACTERIUM TUBERCULOSIS; NEISSERIA GONORRHOEAE; NONHUMAN; NUCLEIC ACID PROBE; NUCLEIC ACID STRUCTURE; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; REVIEW; SENSITIVITY AND SPECIFICITY; YEAST;

HUMANS; INDUSTRIAL MICROBIOLOGY; LABORATORIES; MICROBIOLOGICAL TECHNIQUES; NUCLEIC ACID PROBES; PEPTIDE NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL; TIME FACTORS;

EID: 0036132829     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(01)00340-2     Document Type: Review

References (76)

1. Nucleic acid amplification technologies
2. Identification of individual prokaryotic cells by using enzyme-labeled, rRNA targeted oligonucleotide probes
3. Phylogenetic identification and in situ detection of individual cells without cultivation
4. Use of nucleic acid probes for identification of Mycobacterium tuberculosis directly from MB/BacT bottles
5. Flow cytometric detection of ribosomal RNA in suspended cells by fluorescent in situ hybridization
6. Phylogenetic stains: Ribosomal RNA-based probes for the identification of single cells
7. Stability of peptide nucleic acids in human serum and cellular extracts
8. Differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterial liquid cultures by using peptide nucleic acid-fluorescence in situ hybridization probes
9. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen bonding rules
10. PNA blocker probes enhance specificity in probe assays
11. Self-reporting PNA/DNA primers for PCR analysis
12. Massive parallel genomics
13. The phylogeny of prokaryotes
14. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
15. Yeasts and molds
16. A formula for thermal stability (Tm) prediction of PNA/DNA duplexes
17. PNA solubility enhancers
18. The use of alkaline phosphatase-labeled oligonucleotides probes as culture confirmation reagents for the identification of commercially important bacteria
19. Evaluation of a fluorescence in situ hybridization assay for differentiation between tuberculous and non-tuberculous Mycobacterium species in smears of LJ and MGIT cultures using peptide nucleic acid probes
20. Variability in the stability of DNA-peptide nucleic acid (PNA) single-base mismatched duplexes: Real time hybridization during affinity electrophoresis in PNA-containing gels
21. Automated detection of point mutations by electrophoresis in peptide-nucleic acid-containing gels
22. Rapid and specific detection of PCR products using light-up probes
23. Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA
24. Flow cytometric detection of EBV (EBER snRNA) using peptide nucleic acid probes
25. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures
26. Molecular beacons for double-stranded DNA
27. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences
28. In situ hybridization detection of single-copy human papillomavirus on isolated cells, using a catalyzed signal amplification system: Genpoint™
29. Multicenter evaluation of AMPLICOR and automated COBAS AMPLICOR CT/NG tests for Neisseria gonorhoeae
30. Evaluation of Gen-Probe amplified Mycobacterium tuberculosis direct test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens
31. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
32. Applications of peptide nucleic acids
33. Peptide nucleic acids as therapeutic agents
34. Peptide nucleic acid (PNA): A DNA mimic with a pseudopeptide backbone
35. Peptide Nucleic Acid (PNA), a DNA mimic with a peptide backbone
36. Detection of hybrid formation between peptide nucleic acids and DNA by fluorescence polarization in the presence of polylysine
37. Differentiation between Candida albicans and Candida dubliniensis by fluorescence in situ hybridization using peptide nucleic acid probes
38. Evaluation of a fluorescence hybridization assay using peptide nucleic acid probes for identification and differentiation of tuberculous and non-tuberculous mycobacteria in liquid cultures
39. Fluorescent PNA probes for in situ bacterial identification
40. Peptide nucleic acid pre-gel hybridization: An alternative to southern hybridization
41. Multicolor multiplex microbial identification using PNA probes targeting rRNA
42. Filter-based PNA in situ hybridization for rapid detection, identification and enumeration of specific microorganisms
43. Identification of indicator microorganisms using a standardized PNA FISH method
44. Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water
45. Identification of enterococci by ribotyping with horseradish-peroxidase-labeled 16S rDNA probes
46. Global epidemiology of tuberculosis
47. Solid-phase synthesis of doubly labeled peptide nucleic acids as probes for the real-time detection of hybridization
48. A convergent strategy for the modification of peptide nucleic acids: Novel mismatch-specific PNA-hybridization probes
49. Use of rRNA-targeted oligonucleotide probes for the characterization of the microflora from fermentation of Fontina cheese
50. Dekkera van der Walt
51. Diagnostic applications of PNA oligomers
52. Direct detection and identification of Mycobacterium tuberculosis in smear-positive sputum samples by fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes
53. Fluorescence in situ hybridization assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures
54. A new molecular method for simultaneous identification and enumeration of Brettanomyces in wine using peptide nucleic acid probes
55. Rapid detection, identification, and enumeration of Pseudomonas aeruginosa in bottled water using peptide nucleic acid probes
56. Rapid detection, identification, and enumeration of Escherichia coli using PNA probes and laser scanner
57. Rapid detection, identification, and enumeration of Escherichia coli cells in municipal water by chemiluminescent in situ hybridization
58. Identification of Brettanomyces (Dekkera bruxellensis) from wine by fluorescence in situ hybridization using peptide nucleic acid probes
59. Rapid detection, identification, and enumeration of Escherichia coli by fluorescence in situ hybridization using an array scanner
60. Combination of ATP-bioluminescence and PNA probes allows rapid total counts and identification of specific microorganisms in mixed populations
61. Detection of PCR products in real time using light-up probes
62. Light-up probes: Thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution
63. Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells
64. Detection of immunoglobulin kappa light chain mRNA in paraffin sections by in situ hybridization using peptide nucleic acid probes
65. Application of peptide nucleic acid probes for in situ hybridization
66. Detection of Chlamydia trachomatis infection in urine samples from men and women by Ligase Chain Reaction
67. Candida, Cryptococcus, and other yeasts of medical importance
68. Comparison between Taq DNA polymerase and its Stoffel fragment for quantitative real-time PCR with hybridization probes
69. Mode of action of the antimycobacterial agents and associated aspects of the molecular biology of mycobacteria
70. Bacterial evolution
71. In situ hybridization of Prochlorococcus and Synechococcus (marine cyanobacteria) spp. with rRNA-targeted peptide nucleic acid probes
72. Amplified in situ hybridization with peptide nucleic acid probes for differentiation of Mycobacterium tuberculosis complex and nontuberculous mycobacterium species on formalin-fixed paraffin-embedded archival biopsy and autopsy