Differential expression of apoptotic proteins following hypoxia-induced CREB phosphorylation in the cerebral cortex of newborn piglets

Neurochemical Research

Volumn 32, Issue 7, 2007, Pages 1256-1263

  Delivoria Papadopoulos, Maria ^a,b   Ashraf, Qazi M ^a,b   Mishra, Om Prakash ^a,b  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

^b ST CHRISTOPHER'S HOSPITAL FOR CHILDREN   (United States)

Author keywords

Bad; Bax; Bcl 2; Bcl xl; Brain; CREB protein; Hypoxia; Phosphorylation

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANTIBODY; CREATINE PHOSPHATE; CYCLIC AMP RESPONSIVE ELEMENT BINDING PROTEIN; PROTEIN BAD; PROTEIN BAX; PROTEIN BCL XL; PROTEIN BNIP3; SERINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; APOPTOSIS; ARTICLE; BRAIN CORTEX; BRAIN HYPOXIA; CORRELATION ANALYSIS; CORRELATION COEFFICIENT; HYPOXIA; NEWBORN; NONHUMAN; PIGLET; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; WESTERN BLOTTING;

ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; BCL-ASSOCIATED DEATH PROTEIN; BCL-X PROTEIN; CEREBRAL CORTEX; CYCLIC AMP RESPONSE ELEMENT-BINDING PROTEIN; HYPOXIA, BRAIN; PHOSPHOCREATINE; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS C-BCL-2; RANDOM ALLOCATION; SERINE; SWINE;

EID: 34249276405     PISSN: 03643190     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11064-007-9301-5     Document Type: Article

References (47)

1. Mishra OP, Delivoria-Papadopoulos M (1999) Cellular mechanisms of hypoxic injury in the developing brain. Brain Res Bull 48:233-238
2. Delivoria-Papadopoulos M, Mishra OP (2004) Nuclear mechanisms of hypoxic cerebral injury in the newborn. Clin Perinatol 31:91-105
3. Ghosh A, Greenberg ME (1995) Calcium signaling in neurons: molecular mechanisms and cellular consequences. Science 268:239-247
4. ++-dependent regulation in neuronal gene expression. Curr Opin Neurobiol 7:419-429
5. Hardigham GE, Bading H (1998) Nuclear calcium: a key regulator of gene expression. Biometals 11:345-358
6. Chawla S, Bading H (2001) CREB/CBP and SRE-interacting transcriptional regulators are fast on-off switches: duration of calcium transients specifies the magnitude of transcriptional responses. J Neurochem 79:849-858
7. Fields RD, Esthete F, Stevens B, Itoh K (1997) Action potential-dependent regulation of gene expression: temporal specificity in calcium cyclic AMP-responsice element binding proteins, and mitogen-activated protein kinase signaling. J Neurosci 17:7252-7266
8. Lerea LS, Butler LS, McNamar JO (1992) NMDA and non-NMDA receptor-mediated increase of c-fos mRNA in dentate gyrus neurons involved calcium influx via different routes. J Neurosci 12:2973-2981
9. Bading H, Ginty DD, Greenberg ME (1993) Regulation of gene expression in hippocampal neurons by distinct calcium signaling pathways. Science 260:181-186
10. Lerea LS, McNamara JO (1993) Ionotropic glutamate receptor subtypes activate c-fos transcription by distinct calcium requiring intracellular signaling pathways. Neuron 10:31-41
11. Hardigham GE, Chawla S, Johnson CM, Bading H (1997) Distinct functions of nuclear and cytoplasmic calcium in the control of gene expression. Nature 385:260-265
12. Hardigham GE, Chawla S, Cruzalegui FH, Bading H (1999) Control of recruitment and transcription activating function of CBP determined gene regulation by NMDA receptor and L-type calcium channels. Neuron 22:789-798
13. Dolmetsch RE, Pajvani U, Fife K, Spotts JM, Greenberg ME (2001) Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway. Science 294:333-339
14. ++ response amplitude and duration. Nature 386:855-858
15. ++-influx in neuronal nuclei and cortical synaptosomes of normoxic and hypoxic newborn piglets. Neurosci Lett 318:93-97
16. ++-influxin cerebral cortical neuronal nuclei of newborn piglets. Neurosci Lett 342:119-123
17. ++/calmodulin-dependent protein kinase IV activity during hypoxia in neuronal nuclei from newborn piglets. Neurosci Lett 335:5-8
18. Mishra OP, Ashraf QM, Delivoria-Papadopoulos M (2002) Phosphorylation of cAMP response element binding (CREB) protein during hypoxia in cerebral cortex of newborn piglets and the effect of nitric oxide synthase inhibition. Neuroscience 115:985-991
19. Farlie PG, Dringen R, Rees SM, Kannourakis G, Bernard O (1995) Bcl-2 transgene expression can protect neurons against developmental and induced cell death. Proc Natl Acad Sci USA 92:4397-4401
20. Martinou J-C, Dubois-Dauphin M, Staple JK, Rodriquez I, Frankowski H, Missotten M, Albertini P, Talabot D, Catsicas S, Pietra C, Huarte J (1994) Over expression of Bcl-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia. Neuron 13:1017-1030
21. Oltvai ZN, Milliman CM, Korsmeyer SJ (1993) Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74:609-619
22. Chen J, Zhu RL, Nakayama M, Kawaguchi K, Jin K, Stetler RA, Simon RP, Graham SH (1996) Expression of the apoptosis-effector gene, Bax, is up-regulated in vulnerable hippocampal CA1 neurons following global ischemia. J Neurochem 67:64-71
23. Merry DE, Veis DJ, Hickey WF, Korsmeyer SJ (1994) Bcl-2 protein expression is widespread in the developing nervous system and retained in the adult PNS. Development 120:301-311
24. Reed JC (1996). Mechanisms of Bcl-2 family protein function and dysfunction in health and disease. Behring Inst Mitt 97:72-100
25. Giuffrida AM, Cox D, Mathias AP (1975) RNA polymerase activity invarious classes of nuclei from different regions of rat brain during postnatal development. J Neurochem 24:749-755
26. Austoker J, Cox D, Mathias P (1972) Fractionation of nuclei from brain by zonal centrifugation and a study of ribonucleic acid polymerase activity in the various classes of nuclei. Biochem J 129:1139-1155
27. Lowry OH, Rosenbrough NJ, Farr AL, Randal RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
28. Lamprechet W, Stein P, Heinz F, Weissner H (1974) Creatine phosphate. In: Bergmeyer HU (ed) Methods of enzymatic Analysis, vol 4. Academic Press, New York, pp 1777-1781
29. Frandsen A, Schousboe A (1991) Dantrolene prevents cytotoxicity and Ca++ release from intracellular stores in cultured cerebral cortical neurons. J Neurchem 56:1075-1078
30. Krajewski S, Tanaka S, Takayama S, Schibler MJ, Fenton W, Reed JC (1993) Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. Cancer Res 53:4701-4714
31. Gillardon F, Lenz C, Waschke KF, Krajewski S, Reed JC, Zimmermann M, Kuschinsky W (1996) Altered expression of Bcl-2, Bcl-X, Bax, and c-Fos colocalizes with DNA fragmentation and ischemic cell damage following middle cerebral artery occlusion in rats. Mol Brain Res 40:254-260
32. Jacobson MD, Burne JF, King MP, Miyashita T, Reed JC, Raff MC (1993) Bcl-2 blocks apoptosis in cells lacking mitochondrial DNA. Nature 361:365-369
33. Miyashita T, Krajewski S, Krajewska M, Wang HG, Lin HK, Liebermann DA, Hoffman B, Reed JC (1994) Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene 9:1799-1805
34. Krajewski S, Mal JK, Krajewska M, Sikorska M, Mossakowski MJ (1995) Upregulation of Bax protein levels in neurons following cerebral ischemia. J Neurosci 15:6364-6376
35. Ashraf QM, Zanelli S, Mishra OP, Delivoria-Papadopoulos M (2001) Phosphorylation of Bcl-2 and Bax proteins during hypoxia in newborn piglets. Neurochem Res 26:1-9
36. Hockenbery D, Nunez G, Milliman C, Schreiber RD, Korsmeyer SJ (1990) Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348:334-336
37. 2+. Oncogene 12:2259-2266
38. Kharbandra S, Pandey P, Schofield L, Israels S, Roncinske R, Yoshida K, Bharti A, Yuan ZM, Saxena S, Weichselbaum R (1997) Role of Bcl-xl as an inhibitor of cytosolic cytochrome c accumulation in DNA damage-induced apoptosis. Proc Natl Acad Sci USA 94:6939-6942
39. Kim CN, Wang X, Huang Y, Ibrado AM, Liu L, Fang G, Bhalla K (1997) Over expression of Bcl-X(L) inhibits Ara-C-induced mitochondrial loss of cytochrome c and other perturbations that activate the molecular cascade of apoptosis. Cancer Res 57:3115-3120
40. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X (1997) Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-489
41. Zubrow AB, Delivoria-Papadopoulos M, Ashraf QM, Ballesteros JR, Fritz KI, Mishra OP (2002) Nitric oxide-mediated expression of Bax protein and DNA fragmentation during hypoxia in neuronal nuclei from newborn piglets. Brain Res 954:60-67
42. Huang Z, Huang PL, Panathian N, Dalkara T, Fishman MC, Moskowitz MA (1994) Effects of cerebral ischemiain mice deficient in neuronal nitric oxide synthase. Science 265:1183-1885
43. Yun H-Y, Dawson VL, Dawson TM (1997) Nitric oxide in health and diseases of the nervous system. Mol Psychiatr 2:300-310
44. Numagami Y, Zubrow AB, Mishra OP, Delivoria-Papadopoulos M (1997) Lipid free radical generation and brain cell membrane alteration following nitric oxide synthase inhibition during cerebral hypoxia in the newborn piglet. J Neurochem 69:1542-1547
45. Mishra OP, Zanelli SA, Ohnishi ST, Delivoria-Papadopoulos M (2000) Hypoxia-induced generation of nitric oxide free radicals in cerebral cortex of newborn guinea pigs. Neurochem Res 25:1559-1565
46. Zanelli SA, Ashraf QM, Mishra OP (2002) Nitration is a mechanism of regulation of the NMDA receptor function during hypoxia. Neurosci 112:869-877
47. Truttman AC, Ashraf Q, Mishra OP, Delivoria-Papadopoulos M (2004) Effect of hypoxia on protein phsphatase 2A activity, subcellular distribution and expression in cerebral cortex of newborn piglets. Neuroscience 127:355-363