Identification of protein kinase C as an intermediate in NA, K-atpase beta-subunit mediated lamellipodia formation and suppression of cell motility in carcinoma cells

Cellular and Molecular Biology

Volumn 52, Issue 8, 2006, Pages 41-47

  Barwe, S P ^a   Rajasekaran, S A ^a   Rajasekaran, A K ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cell attachment; Cell motility; K ATPase; Na; PI3 Kinase; PKC

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); BISINDOLYLMALEIMIDE; LIPOCORTIN 2; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE C; RAC1 PROTEIN;

ANIMAL CELL; ARTICLE; BETA CHAIN; CANCER GROWTH; CANCER INHIBITION; CARCINOMA CELL; CELL ADHESION; CELL MOTILITY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INHIBITION; GROWTH INHIBITION; LAMELLIPODIUM; MOLONEY MURINE SARCOMA ONCOVIRUS; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION;

1-PHOSPHATIDYLINOSITOL 3-KINASE; ANIMALS; ANNEXIN A2; CELL ADHESION; CELL LINE, TRANSFORMED; CELL LINE, TUMOR; CELL MOVEMENT; DOGS; ENZYME ACTIVATION; MUTATION; NA(+)-K(+)-EXCHANGING ATPASE; OUABAIN; PROTEIN BINDING; PROTEIN KINASE C; PROTEIN SUBUNITS; PSEUDOPODIA; RAC1 GTP-BINDING PROTEIN;

MAIZE STREAK VIRUS; MOLONEY MURINE SARCOMA VIRUS;

EID: 34249105226     PISSN: 01455680     EISSN: 1165158X     Source Type: Journal    
DOI: 10.1170/T759     Document Type: Article

References (26)

1. (+)-ATPase alpha-subunit. Biochemistry. 1993, 32: 3511-3518.
2. Barwe, S.P., Anilkumar, G., Moon, S.Y., Zheng, Y., Whitelegge, J.P., Rajasekaran, S.A. and Rajasekaran, A.K., Novel role for Na,K-ATPase in phosphatidylinositol 3-kinase signaling and suppression of cell motility. Mol Biol Cell. 2005, 16: 1082-1094.
3. Chow, D.C. and Forte, J.G., Functional significance of the beta-subunit for heterodimeric P-type ATPases. J Exp Biol. 1995, 198: 1-17.
4. Efendiev, R. and Pedemonte, C.H., Contrary to Rat-Type, Human-Type Na,K-ATPase Is Phosphorylated at the Same Amino Acid by Hormones that Produce Opposite Effects on Enzyme Activity. J Am Soc Nephrol. 2006, 17: 31-38.
5. Geering, K., The functional role of beta subunits in oligomeric P-type ATPases. JBioenerg Biomembr. 2001, 33: 425-438.
6. Gentili, C., Morelli, S. and Russo De Boland, A., Involvement of PI3-kinase and its association with c-Src in PTH-stimulated rat enterocytes. J Cell Biochem. 2002, 86: 773-783.
7. Haas, M., Wang, H., Tian, J. and Xie, Z., Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J Biol Chem. 2002, 277: 18694-18702.
8. Huang, K.P., The mechanism of protein kinase C activation. Trends Neurosci. 1989, 12:425-432.
9. Hynes, R.O., Integrins: bidirectional, allosteric signaling machines. Cell. 2002, 110: 673-687.
10. Kazanietz, M.G., Caloca, M.J., Aizman, O. and Nowicki, S., Phosphorylation of the catalytic subunit of rat renal Na+, K+-ATPase by classical PKC isoforms. Arch Biochem Biophys. 2001, 388: 74-80.
11. Koob, R., Kraemer, D., Trippe, G., Aebi, U. and Drenckhahn, D., Association of kidney and parotid Na+, K(+)-ATPase microsomes with actin and analogs of spectrin and ankyrin. Eur J Cell Biol. 1990, 53: 93-100.
12. Nelson, W.J. and Veshnock, P.J., Ankyrin binding to (Na+ + K+)ATPase and implications for the organization of membrane domains in polarized cells. Nature. 1987, 328: 533-536.
13. Palmer, S., Groves, N., Schindeler, A., Yeoh, T., Biben, C., Wang, C.C., et al, The small muscle-specific protein Csl modifies cell shape and promotes myocyte fusion in an insulin-like growth factor 1-dependent manner. J Cell Biol. 2001, 153: 985-998.
14. Parker, P.J. and Murray-Rust, J., PKC at a glance. J Cell Sci. 2004, 117: 131-132.
15. Pedemont, C.H. and Bertorello, A.M., Short-term regulation of the proximal tubule Na+,K+-ATPase: increased/decreased Na+,K+-ATPase activity mediated by protein kinase C isoforms. J Bioenerg Biomembr. 2001, 33: 439-447.
16. Rajasekaran, S.A., Barwe, S.P. and Rajasekaran, A.K., Multiple functions of Na,KATPase in epithelial cells. Semin Nephrol. 2005, 25: 328-334.
17. Rajasekaran, S.A., Gopal, J., Willis, D., Espineda, C., Twiss, J.L. and Rajasekaran, A.K., Na,K-ATPase beta1-subunit increases the translation efficiency of the alpha1-subunit in MSV-MDCK cells. Mol Biol Cell. 2004, 15: 3224-3232.
18. Rajasekaran, S.A., Palmer, L.G., Quan, K., Harper, J.F., Ball, W.J., Jr., Bander, N.H., et al, Na,K-ATPase beta-subunit is required for epithelial polarization, suppression of invasion, and cell motility. Mol Biol Cell. 2001, 12: 279-295.
19. Ridley, A.J., Schwartz, M.A., Burridge, K., Firtel, R.A., Ginsberg, M.H., Borisy, G., et al, Cell migration: integrating signals from front to back. Science. 2003, 302: 1704-1709.
20. Sava, G., Frausin, F., Cocchietto, M., Vita, F., Podda, E., Spessotto, P., et al, Actindependent tumour cell adhesion after short-term exposure to the antimetastasis ruthenium complex NAMI-A. Eur J Cancer. 2004, 40: 1383-1396.
21. Schindeler, A., Lavulo, L. and Harvey, R.P., Muscle costameric protein, Chisel/Smpx, associates with focal adhesion complexes and modulates cell spreading in vitro via a Rac1/p38 pathway. Exp Cell Res. 2005, 307: 367-380.
22. Sun, Y. and Ball, W.J., Jr., Identification of antigenic sites on the Na+/K(+)-ATPase beta-subunit: their sequences and the effects of thiol reduction upon their structure. Biochim Biophys Acta. 1994, 1207: 236-248.
23. Tian, J., Cai, T., Yuan, Z., Wang, H., Liu, L., Haas, M., et al, Binding of Src to Na+/K+-ATPase Forms a Functional Signaling Complex. Mol Biol Cell. 2006, 17: 317-326.
24. Xie, Z. and Cai, T., Na+-K+ - ATPase-mediated signal transduction: from protein interaction to cellular function. Mol Interv. 2003, 3: 157-168.
25. Yuan, Z., Cai, T., Tian, J., Ivanov, A.V., Giovannucci, D.R. and Xie, Z., Na/K-ATPase tethers phospholipase C and IP3 receptor into a calcium-regulatory complex. Mol Biol Cell. 2005, 16: 4034-4045.
26. Yudowski, G.A., Efendiev, R., Pedemonte, C.H., Katz, A.I., Berggren, P.O. and Bertorello, A.M., Phosphoinositide-3 kinase binds to a proline-rich motif in the Na+, K+-ATPase alpha subunit and regulate its trafficking. Proc Natl Acad Sci USA. 2000, 97:6556-6561.