Molecular cloning and characterization of high-affinity nitrate transporters in marine phytoplankton

Journal of Phycology

Volumn 43, Issue 3, 2007, Pages 542-552

  Song, Bongkeun ^a   Ward, Bess B ^b  

^a UNIVERSITY OF NORTH CAROLINA WILMINGTON   (United States)

^b PRINCETON UNIVERSITY   (United States)

Author keywords

Diatom; Dunaliella tertiolecta; Emiliania huxleyi; High affinity nitrate transporter genes; Nitrate uptake

Indexed keywords

BACILLARIOPHYCEAE; BACILLARIOPHYTA; CHAETOCEROS MUELLERII; CHLOROPHYCEAE; DITYLUM BRIGHTWELLII; DUNALIELLA TERTIOLECTA; EMILIANIA HUXLEYI; HAPTOPHYCEAE; SKELETONEMA COSTATUM; THALASSIOSIRA WEISSFLOGII;

EID: 34249073036     PISSN: 00223646     EISSN: 15298817     Source Type: Journal    
DOI: 10.1111/j.1529-8817.2007.00352.x     Document Type: Article

References (29)

1. Cerezo, M., Tillard, P., Filleur, S., Munos, S., Daniel-Vedele, F. Gojon, A. 2001. Major alterations of the regulation of root NO3-uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in Arabidopsis. Plant Physiol. 127: 262 71.
2. Collos, Y. 1982. Transient situation in nitrate assimilation by marine diatoms. 3. Short-term uncoupling of nitrate uptake and reduction. J. Exp. Mar. Biol. Ecol. 62: 285 95.
3. Collos, Y., Vaquer, A. Souchu, P. 2005. Acclimation of nitrate uptake by phytoplankton to high substrate levels. J. Phycol. 41: 466 78.
4. DeManche, J. M., Curl, H. C., Jr., Lundy, D. W. Donaghay, P. L. 1979. The rapid response of the marine diatom Skeletonema costatum to changes in external and internal nutrient concentration. Mar. Biol. 53: 323 33.
5. Dortch, Q. 1982. Effect of growth conditions on accumulation of internal nitrate, ammonium, amino acids, and protein in three marine diatoms. J. Exp. Mar. Biol. Ecol. 61: 243 64.
6. Dortch, Q. 1990. The interaction between ammonium and nitrate uptake in phytoplankton. Mar. Ecol. Prog. Ser. 61: 183 201.
7. Dortch, Q., Clayton, J. R., Thoresen, S. S. Ahmed, S. I. 1984. Species differences in accumulation of nitrogen pools in phytoplankton. Mar. Biol. 81: 237 50.
8. Dortch, Q. Conway, H. I. 1984. Interactions between nitrate and ammonium uptake: variation with growth rate, nitrogen source and species. Mar. Biol. 79: 151 64.
9. Dortch, Q., Thompson, P. A. Harrison, P. J. 1991a. Short-term interaction between nitrate and ammonium uptake in Thalassiosira pseudonana: effect of preconditioning nitrogen source and growth rate. Mar. Biol. 110: 183 93.
10. Dortch, Q., Thompson, P. A. Harrison, P. J. 1991b. Variability in nitrate uptake kinetics in Thalassiosira pseudonana (Bacillariophyceae). J. Phycol. 27: 35 9.
11. Eppley, R. W., Coatsworth, J. L. Solorzano, L. 1969. Studies of nitrate reductase in marine phytoplankton. Limnol. Oceanogr. 14: 194 205.
12. Filleur, S., Dorbe, M. F., Cerezo, M., Orsel, M., Granier, F., Gojon, A. Daniel-Vedele, F. 2001. An Arabidopsis T-DNA mutant affected in Nrt2 genes is impaired in nitrate uptake. FEBS Lett. 489: 220 4.
13. Forde, B. G. 2000. Nitrate transporters in plants: structure, function and regulation. Biochim. Biophys. Acta 1465: 219 35.
14. Galván, A. Fernández, E. 2001. Eukaryotic nitrate and nitrite transporters. Cell. Mol. Life Sci. 58: 225 33.
15. Harris, G. P. 1986. Phytoplankton Ecology: Structure, Function and Fluctuation. Chapman & Hall, New York, pp. 384.
16. He, Q., Qiao, D., Zhang, Q., Li, Y., Xu, H., Wei, L., Gu, Y. Cao, Y. 2004. Cloning and expression study of a putative high-affinity nitrate transporter gene from Dunaliella salina. J. Appl. Phycol. 16: 395 400.
17. Hildebrand, M. Dahlin, K. 2000. Nitrate transporter genes from the diatom Cylindrotheca fusiformis (Bacillariophyceae): mRNA levels controlled by nitrogen source and by the cell cycle. J. Phycol. 36: 702 13.
18. Koltermann, M., Moroni, A., Gazzarini, S., Nowara, D. Tischner, R. 2003. Cloning, functional expression and expression studies of the nitrate transporter gene from Chlorella sorokiniana (strain 211-8k). Plant Mol. Biol. 52: 855 64.
19. Kyte, J. Doolittle, R. F. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157: 105 32.
20. Lomas, M. W. Glibert, P. M. 1999. Interactions between NH4+ and NO3- uptake and assimilation: comparison of diatoms and dinoflagellates at several growth temperatures. Mar. Biol. 133: 541 51.
21. Lomas, M. W. Glibert, P. M. 2000. Comparisons of nitrate uptake, storage, and reduction in marine diatoms and flagellates. J. Phycol. 36: 903 13.
22. McCarthy, J. J., Taylor, W. R. Taft, J. L. 1975. The dynamics of nitrogen and phosphorous cycling in the open waters of the Chesapeake Bay. In Church, T. M. Ed. Marine Chemistry in the Coastal Environment. American Chemical Society, Washington, D.C., pp. 664 81.
23. Navarro, M. T., Prieto, R., Fernandez, E. Galvan, A. 1996. Constitutive expression of nitrate reductase changes the regulation of nitrate and nitrite transporters in Chlamydomonas reinhardtii. Plant J. 9: 819 27.
24. Poulsen, N. Kröger, N. 2005. A new molecular tool for transgenic diatoms: control of mRNA and protein biosynthesis by an inducible promoter-terminator cassette. FEBS J. 272: 3413 23.
25. Price, N. M., Harrison, G. I., Hering, J. G., Hudson, R. J., Nirel, P. M. V., Palenik, B. Morel, F. M. M. 1988. Preparation and chemistry of the artificial algal culture medium Aquil. Biol. Oceanogr. 6: 443 61.
26. Saitou, N. Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. J. Mol. Evol. 4: 406 25.
27. Song, B. Ward, B. B. 2004. Molecular characterization of the assimilatory nitrate reductase gene and its expression in the marine green alga Dunaliella tertiolecta (Chlorophyceae). J. Phycol. 40: 721 31.
28. Swofford, D. L. 2002. PAUP*: Phylogenetic Anaylsis Using Parsimony (and Other Methods). Sinauer, Sunderland, Massachusetts.
29. Yin, K., Harrison, P. J. Dortch, Q. 1998. Lack of ammonium inhibition of nitrate uptake for a diatom grown under low light conditions. J. Exp. Mar. Biol. Ecol. 228: 151 65.