Short peptide tags increase the yield of C-terminally labeled protein

Biotechnology Letters

Volumn 29, Issue 7, 2007, Pages 1065-1073

  Kobayashi, Teruaki ^a,b   Shiratori, Miwa ^a   Nakano, Hirofumi ^a   Eguchi, Chikashi ^a   Shirai, Makoto ^b   Naka, Daiji ^a   Shibui, Tatsurou ^a  

^a ZOEGENE Corporation   (Japan)

^b IBARAKI UNIVERSITY   (Japan)

Author keywords

Cell free translation; Fluorescence; Protein labeling; Puromycin

Indexed keywords

C-TERMINAL PROTEIN; CELL-FREE TRANSLATION; PROTEIN LABELING; PUROMYCIN;

AMINO ACIDS; CELLS; ENZYMES; FLUORESCENCE;

PEPTIDES;

GLUTATHIONE TRANSFERASE; PEPTIDE; PUROMYCIN; RIBOSOMAL PROTEIN S30; RIBOSOME PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CHEMISTRY; ESCHERICHIA COLI; HUMAN; IMMUNOPRECIPITATION; METABOLISM; RABBIT; RETICULOCYTE; SEQUENCE ANALYSIS;

ANIMALS; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; HUMANS; IMMUNOPRECIPITATION; PEPTIDES; PUROMYCIN; RABBITS; RETICULOCYTES; RIBOSOMAL PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

EID: 34249035271     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9362-1     Document Type: Article

References (21)

1. Agafonov DE, Rabe KS, Grote M, Voertler CS, Sprinzl M (2006) C-Terminal modifications of a protein by UAG-encoded incorporation of puromycin during in vitro protein synthesis in the absence of release factor 1. Chembiochemistry 7:330-336
2. Ahn JH, Chu HS, Kim TW, Oh IS, Choi CY, Hahn GH, Park CG, Kim DM (2005) Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions. Biochem Biophys Res Commun 338:1346-1352
3. Azzam ME, Algranati ID (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent protein chains from polysomes. Proc Natl Acad Sci USA 70:3866-3869
4. Doi N, Takashima H, Kinjo M, Sakata K, Kawahashi Y, Oishi Y, Oyama R, Miyamoto-Sato E, Sawasaki T, Endo Y, Yanagawa H (2002) Novel fluorescence labeling and high-throughput assay technologies for in vitro analysis of protein interactions. Genome Res 12:487-492
5. Fedorov AN, Baldwin TO (1995) Contribution of cotranslational folding to the rate of formation of native protein structure. Proc Natl Acad Sci USA 92:1227-1231
6. Figeys D (2003) Novel approaches to map protein interactions. Curr Opin Biotechnol 14:119-125
7. Hanes J, Pluckthun A (1997) In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci USA 94:4937-4942
8. Hirashima A, Kaji A (1973) Role of elongation factor G and a protein factor on the release of ribosomes from messenger ribonucleic acid. J Biol Chem 248:7580-7587
9. Maden BE (2003) Historical review: Peptidyl transfer, the Monro era. Trends Biochem Sci 28:619-624
10. Madin K, Sawasaki T, Ogasawara T, Endo Y (2000) A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes. Proc Natl Acad Sci USA 97:559-564
11. Miyamoto-Sato E, Takashima H, Fuse S, Sue K, Ishizaka M, Tateyama S, Horisawa K, Sawasaki T, Endo Y, Yanagawa H (2003) Highly stable and efficient mRNA templates for mRNA-protein fusions and C-terminally labeled proteins. Nucleic Acids Res 31:e78
12. Nemoto N, Miyamoto-Sato E, Yanagawa H (1999) Fluorescence labeling of the C-terminus of proteins with a puromycin analogue in cell-free translation systems. FEBS Lett 462:43-46
13. Oyama R, Takashima H, Yonezawa M, Doi N, Miyamoto-Sato E, Kinjo M, Yanagawa H (2006) Protein-protein interaction analysis by C-terminally specific fluorescence labeling and fluorescence cross-correlation spectroscopy. Nucleic Acids Res 34:e102
14. Petrou PS, Georgiou S, Christofidis I, Kakabakos SE (2002) Increased sensitivity of heterogeneous fluoroimmunoassays employing fluorescein-labeled antibodies by simple treatment of the wells with glycerin solution. J Immunol Methods 266:175-179
15. Piehler J (2005) New methodologies for measuring protein interactions in vivo and in vitro. Curr Opin Struct Biol 15:4-14
16. Sawasaki T, Ogasawara T, Morishita R, Endo Y (2002) A cell-free protein synthesis system for high-throughput proteomics. Proc Natl Acad Sci USA 99:14652-14657
17. Starck SR, Green HM, Alberola-Ila J, Roberts RW (2004) A general approach to detect protein expression in vivo using fluorescent puromycin conjugates. Chem Biol 11:999-1008
18. Stewart JP, Silvia OJ, Atkin IM, Hughes DJ, Ebrahimi B, Adler H (2003) In vivo function of a gammaherpesvirus virion glycoprotein: influence on B-cell infection and mononucleosis. J Virol 78:10449-10459
19. Tabuchi I (2003) Next-generation protein-handling method: puromycin analogue technology. Biochem Biophys Res Commun 305:1-5
20. Tan LP, Chen GY, Yao SQ (2004) Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorg Med Chem Lett 14:5735-5738
21. Yamaguchi J, Nemoto N, Sasaki T, Tokumasu A, Mimori-Kiyosue Y, Yagi T, Funatsu T (2001) Rapid functional analysis of protein-protein interactions by fluorescent C-terminal labeling and single-molecule imaging. FEBS Lett 502:79-83