Promoter methylation, mutation, and genomic deletion are involved in the decreased NDRG2 expression levels in several cancer cell lines

Biochemical and Biophysical Research Communications

Volumn 358, Issue 1, 2007, Pages 164-169

  Liu, Na ^a   Wang, Lifeng ^a   Liu, Xinping ^a   Yang, Qi ^a   Zhang, Jing ^a   Zhang, Wei ^a   Wu, Yousheng ^a   Shen, Lan ^a   Zhang, Yingqi ^a   Yang, Angang ^a   Han, Hua ^a   Zhang, Jian ^a   Yao, Libo ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Loss of heterozygosity; Methylation; NDRG2; PCR SSCP; Promoter alteration

Indexed keywords

MESSENGER RNA;

ARTICLE; BREAST CANCER; CANCER CELL CULTURE; CONTROLLED STUDY; GENE DELETION; GENE IDENTIFICATION; GENE MUTATION; GENE SEQUENCE; GLIOMA; HETEROZYGOSITY; HUMAN; HUMAN TISSUE; METHYLATION; NDRG2 GENE; NUCLEOTIDE SEQUENCE; ONCOGENE C MYB; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TUMOR SUPPRESSOR GENE; WESTERN BLOTTING;

BASE SEQUENCE; CELL LINE, TUMOR; DNA METHYLATION; GENE DELETION; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; PROMOTER REGIONS (GENETICS); TUMOR SUPPRESSOR PROTEINS;

EID: 34248185677     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.04.089     Document Type: Article

References (21)

1. Deng Y.C., Yao L.B., Liu X.P., Nie X.Y., Wang J.C., Zhang X.G., and Su C.Z. Exploring a new gene containing ACP like domain in human brain and expression it in E. coli. Prog. Bichem. Biophys. 28 (2001) 72-76
2. Zhou R.H., Kokame K., Tsukamoto Y., Yutani C., Kato H., and Miyata T. Characterization of the human NDRG gene family: a newly identified member, NDRG4, is specifically expressed in brain and heart. Genomics 73 (2001) 86-97
3. Deng Y., Yao L., Chau L., Ng S.S., Peng Y., Liu X., Au W.S., Wang J., Li F., Ji S., Han H., Nie X., Li Q., Kung H.F., Leung S.Y., and Lin M.C. N-Myc downstream-regulated gene 2 (NDRG2) inhibits glioblastoma cell proliferation. Int. J. Cancer 106 (2003) 342-347
4. Wu G.Q., Liu X.P., Wang L.F., et al. Induction of apoptosis of HepG2 cells by NDRG2. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 19 (2003) 357-360
5. Choi S.C., Kim K.D., and Kim J.T. Expression and regulation of NDRG2 (N-myc downstream regulated gene 2) during the differentiation of dendritic cells. FEBS Lett. 553 (2003) 413-418
6. Mitchelmore C., Buchmann-Moller S., Rask L., West M.J., Troncoso J.C., and Jensen N.A. NDRG2: a novel Alzheimer's disease associated protein. Neurobiol. Dis. 16 (2004) 48-58
7. Boulkroun S., Fay M., Zennaro M.C., Escoubet B., Jaisser F., Blot-Chabaud M., Farman N., and Courtois-Coutry N. Characterization of rat NDRG2 (N-Myc downstream regulated gene 2), a novel early mineralocorticoid-specific induced gene. J. Biol. Chem. 277 (2002) 31506-31515
8. Burchfield J.G., Lennard A.J., Narasimhan S., Hughes W.E., et al. Akt mediates insulin stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta. J. Biol. Chem. 279 (2004) 18623-18632
9. Li J., Liu X.P., Lin S.X., Deng Y.C., Meng Q.J., Zhang W., Li S.J., Nie X.Y., and Yao L.B. Expression pattern of ndr2 gene, A candidate tumor-suppressor, in different human tissues and tumors. Prog. Biochem. Biophys. 29 (2002) 223-227
10. Lusis E.A., Watson M.A., Chicoine M.R., Lyman M., Roerig P., Reifenberger G., Gutmann D.H., and Perry A. Integrative genomic analysis identifies NDRG2 as a candidate tumor suppressor gene frequently inactivated in clinically aggressive meningioma. Cancer Res. 65 (2005) 7121-7126
11. Shao W.Z., Zheng Y.-S., Li J.-H., et al. The establishing and biological characterization of human glioma cell BT325. China J. Neurosurg. 4 (1988) 103-110
12. Tong D., Kucera E., Schuster E., Schmutzler R.K., Swoboda H., Reinthaller A., Leodolter S., and Zeillinger R. Loss of heterozygosity (LOH) at p53 is correlated with LOH at BRCA1 and BRCA2 in various human malignant tumors. Int. J. Cancer 88 (2000) 319-322
13. Manel E. CpG island hypermethylation and tumor suppressor genes: a booming present, a brighter future. Oncogene 21 (2002) 5427-5440
14. Boyes J., and Bird A. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein. Cell 64 (1991) 1123-1134
15. Gonzalez-Zulueta M., Bender C.M., Yang A.S., Nguyen T., Beart R.W., Van Tornout J.M., and Jones P.A. Methylation of the 5′ CpG island of the p16/CDKN2 tumor suppressor gene in normal and transformed human tissues correlates with gene silencing. Cancer Res. 55 (1995) 4531-4535
16. Dobrovic A., and Simpfendorfer D. Methylation of the BRCA1 gene in sporadic breast cancer. Cancer Res. 57 (1997) 3347-3350
17. Kane M.F., Loda M., Gaida G.M., Lipman J., Mishra R., Goldman H., Jessup J.M., and Kolodner R. Methylation of the hMLH1 promoter correlates with lack of expression of hMLH1 in sporadic colon tumors and mismatch repair-defective human tumor cells. Cancer Res. 7 (1997) 808-881
18. Pollock P.M., Stark M.S., and Palmer J.M. Mutation analysis of the CDKN2A promoter in Australian Melanoma families. Genes Chromosomes Cancer 32 (2001) 89-94
19. Liu L., Dilworth D., Gao L., Monzon J., Summers A., Lassam N., and Hogg D. Mutation of the CDKN2A 59 UTR creates an aberrant initiation codon and predisposes to melanoma. Nat. Genet. 21 (1999) 128-132
20. Yamada K., Yuan X., Ishiyama S., Ichikawa F., Kohno S., Shoji S., Hayashi H., and Nonaka K. Identification of a single nucleotide insertion polymorphism in the upstream region of the insulin promoter factor-1 gene: an association study with diabetes mellitus. Diabetologia 41 (1998) 603-605
21. Zhang J., Li F., Liu X., Shen L., Liu J., Su J., and Yao L. The repression of human differentiation related gene NDRG2 expression by MYC via MIZ-1 dependent interaction with the NDRG2 core promoter. J. Biol. Chem. 281 51 (2006) 39159-39168