A cell-based assay system for high-throughput screening of anti-wrinkle agents in human dermal fibroblast transfectant cells

Biotechnology and Applied Biochemistry

Volumn 47, Issue 1, 2007, Pages 27-31

  Huh, Sungran ^a   Lee, Jongsung ^a,b   Jung, Eunsun ^a   Ham, Yeonggeun ^a   Sang, Suk Kim ^c   Chang, Gu Hyun ^a   Yeong, Shik Kim ^b   Park, Deokhoon ^a  

^a Biospectrum Life Science Institute   (South Korea)

^b Seoul National University   (South Korea)

^c Skincure Life Science Institute   (South Korea)

Author keywords

Cell based assay; Human dermal fibroblast transfectant cell; Luciferase; Transfection; Transforming growth factor; Type I collagen promoter

Indexed keywords

ENZYME ACTIVITY; FIBROBLASTS; GENE EXPRESSION; NUCLEIC ACIDS; PLASMA (HUMAN);

CELL BASED ASSAYS; COLLAGEN PROMOTERS; HUMAN DERMAL FIBROBLAST TRANSFECTANT CELLS; LUCIFERASE; TRANSFORMING GROWTH FACTORS;

BIOASSAY;

ANTIBIOTIC G 418; DERMATOLOGICAL AGENT; KANAMYCIN KINASE; LUCIFERASE; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA; ALPHA 2(I) COLLAGEN; COLLAGEN; TRANSFORMING GROWTH FACTOR BETA1; UNCLASSIFIED DRUG;

ANTIBIOTIC RESISTANCE; ARTICLE; CELL ASSAY; COLIA2 GENE; CONTROLLED STUDY; GENE; GENE ACTIVITY; GENE EXPRESSION; GENETIC TRANSFECTION; HIGH THROUGHPUT SCREENING; HOST CELL; HUMAN; HUMAN CELL; PROMOTER REGION; REPORTER GENE; SKIN FIBROBLAST; WRINKLE; CELL CULTURE; CUTANEOUS PARAMETERS; DRUG EFFECT; FIBROBLAST; GENETICS; METABOLISM;

CELLS, CULTURED; COLLAGEN; FIBROBLASTS; GENES, REPORTER; HUMANS; LUCIFERASES; PROMOTER REGIONS (GENETICS); SKIN AGING; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA1; TUMOR NECROSIS FACTOR-ALPHA;

EID: 34247854981     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20060122     Document Type: Article

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