N,N,N-trimethylglycine (betaine) improves analysis of CDR3 diversification in children reconstituting their immune repertoire after hematopoietic stem-cell transplantation [2]

Transplantation

Volumn 83, Issue 7, 2007, Pages 996-997

  Terranova, M Paola ^a   Di Michele, Paola ^a   Scuderi, Francesca ^a   Valetto, Angelo ^b   Martino, Daniela D ^a  

^a G GASLINI INSTITUTE   (Italy)

^b UNIVERSITY OF PISA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BETAINE;

AGAR GEL ELECTROPHORESIS; AUTOTRANSPLANTATION; B LYMPHOCYTE; FINGER DERMATOGLYPHICS; GENE AMPLIFICATION; GRAFT VERSUS HOST REACTION; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; IMMUNOSUPPRESSIVE TREATMENT; LETTER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BETAINE; CHILD; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMANS; LEUKEMIA, LYMPHOCYTIC, ACUTE; POLYMERASE CHAIN REACTION; RECEPTOR-CD3 COMPLEX, ANTIGEN, T-CELL;

EID: 34247584521     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/01.tp.0000258728.02932.02     Document Type: Letter

References (10)

1. Di Martino D, Dallorso S, Terranova P, et al. B-cell repertoire reconstitution after hematopoietic stem cell transplantation in children evaluated by immunoglobulin heavy chain third complementarities determining region fingerprinting. Haematologica 2004; 89: 506.
2. Gokmen E, Raaphorst FM, Boldt DH, et al. Ig heavy chain third complementarity determining regions (HCDR3s) after stem cell transplantation do not resemble the developing human fetal HCDR3 in size distribution and Ig gene utilization. Blood 1998; 92: 2802.
3. H6 immunoglobulin M repertoire reconstitution after haematopoietic stem cell transplantation in children. Transplantation 2005; 79: 98.
4. Saiki RK, Gelfand DH, Stoffel S, et al. Primer-directed enzymatic amplification of DNA with a thermo stable DNA polymerase. Science 1988; 239: 487.
5. Smith, TK, Long CM, Bowman B, et al. Using co solvents to enhance PCR amplification. Amplifications 1990; 5: 16.
6. Chakrabarti R, Shutt CE. The enhancement of PCR amplification by low molecular weight amides. Nucleic Acids Res 2001; 29: 2377.
7. Sarkar G, Kapelner S, Sommer SS. Formamide can dramatically improve the specificity of PCR. Nucleic Acids Res 1990; 18: 7465.
8. Varadaraj K, Skinner DM. Denaturants or co solvents improve the specificity of PCR amplification of a G + C rich DNA using genetically engineered DNA polymerases. Gene 1994; 140: 1.
9. Rees WA, Yager TD, Korte J, et al. Betaine can eliminate the base pair composition dependence of DNA Melting. Biochemistry 1993; 32: 137.
10. Henke W, Herdel K, Jung K, et al. Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res 1997; 25: 3957.