One-step purification of histone deacetylase from Escherichia coli cell-lysate by counter-current chromatography using aqueous two-phase system

Journal of Chromatography A

Volumn 1151, Issue 1-2, 2007, Pages 158-163

  Shibusawa, Yoichi ^a   Takeuchi, Naoko ^a   Tsutsumi, Kanako ^a   Nakano, Shigeru ^a   Yanagida, Akio ^a   Shindo, Heisaburo ^a   Ito, Yoichiro ^b  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Aqueous aqueous two phase systems; Counter current chromatography; Maltose binding protein tagged histone deacetylase; Proteins

Indexed keywords

BINDING SITES; ESCHERICHIA COLI; MALTOSE; POLYETHYLENE GLYCOLS; PROTEINS; PURIFICATION; SOLUTIONS;

CELL LYSATE; COUNTER CURRENT CHROMATOGRAPHY (CCC); HISTONE DEACETYLASE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POTASSIUM PHOSPHATE BUFFER;

ENZYME KINETICS;

AQUEOUS SOLUTION; ARTICLE; CELL LYSATE; COUNTER CURRENT CHROMATOGRAPHY; ESCHERICHIA COLI; EVALUATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PURIFICATION; ULTRAFILTRATION;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COUNTERCURRENT DISTRIBUTION; DEXTRANS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HISTONE DEACETYLASES; POLYETHYLENE GLYCOLS;

ESCHERICHIA COLI;

EID: 34247551620     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2007.01.111     Document Type: Article

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