Purification of alcohol dehydrogenase from bovine liver crude extract by dye-ligand affinity counter-current chromatography

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 799, Issue 2, 2004, Pages 239-244

  Shibusawa, Yoichi ^a   Fujiwara, Takiko ^a   Shindo, Heisaburo ^a   Ito, Yoichiro ^b  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Alcohol dehydrogenase; Dye ligand affinity counter current chromatography; Purification

Indexed keywords

ALCOHOLS; ELECTROPHORESIS; ENZYMES; GELATION; PH EFFECTS; PURIFICATION; SOLVENT EXTRACTION;

CRUDE BOVINE LIVER;

CHROMATOGRAPHIC ANALYSIS;

ALCOHOL DEHYDROGENASE; BUFFER; DEXTRAN; DYE; LIGAND; LIVER EXTRACT; LIVER PROTEIN; MACROGOL; MACROGOL 10000; POTASSIUM DIHYDROGEN PHOSPHATE;

AFFINITY CHROMATOGRAPHY; ALKALINITY; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CATTLE; CENTRIFUGE; COUNTER CURRENT CHROMATOGRAPHY; ELUTION; ENZYME ACTIVITY; ENZYME PURIFICATION; LIVER HOMOGENATE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

BOVINAE;

EID: 0345528160     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2003.10.039     Document Type: Article

References (14)

1. Bohme H.-J., Kopperschlager G., Schulz J., Hofmann E. J. Chromatogr. 69:1972;209.
2. Diezel W., Bohme H.-J., Nissler K., Freyer R., Heilmann W., Kopperschlager G., Hofmann E. Eur. J. Biochem. 38:1973;479.
3. J. Kirchberger, H.-J. Bohme, in: M. Kastner (Ed.), Protein Liquid Chromatography, J. Chromatogr. Library 61 (2000) 415.
4. Ito Y., Bowman R.L. Science. 167:1970;281.
5. Y. Ito, in: N.B. Mandava, Y. Ito (Eds.), Counter-Current Chromatography: Theory and Practice, Marcel Dekker, New York, 1988, Chapter 3, p. 79.
6. W.D. Conway, Countercurrent Chromatography: Principle, Apparatus and Applications, VCH, Weinheim, 1990.
7. Kendall D., Booth A.J., Levy M.S., Lye G.J. Biotechnol. Lett. 23:2001;613.
8. Ito Y., Yang F., Fitze P.E., Sullivan J.V. J. Liq. Chromatogr. Related Technol. 26:2003;1355.
9. Ito Y., Oka H., Slemp J.L. J. Chromatogr. 463:1989;305.
10. Bhatnagar M., Oka H., Ito Y. J. Chromatogr. 463:1989;317.
11. Shibusawa Y., Ito Y. J. Chromatogr. 550:1991;695.
12. Shibusawa Y., Ito Y. J. Liq. Chromatogr. 15:1992;2787.
13. R.K. Bonnichsen, N.G. Brink, in: S.P. Colowick, N.O. Kaplan (Eds.), Method in Enzymology, vol. 1, Academic Press, New York, 1955, p. 495.
14. Laemmli U.K. Nature. 227:1970;680.