Characterization of the sporulation control protein SsgA by use of an efficient method to create and screen random mutant libraries in streptoniycetes

Applied and Environmental Microbiology

Volumn 73, Issue 7, 2007, Pages 2085-2092

  Traag, Bjørn A ^a   Seghezzi, Nicolas ^a,b   Vijgenboom, Erik ^a   Van Wezel, Gilles P ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

^b UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELL DIVISION; FILAMENTOUS ACTINOMYCETES; MICROLITER PLATES; STREPTOMYCETES;

AMINO ACIDS; BIOMASS; CELL GROWTH; MUTAGENESIS; SUBSTITUTION REACTIONS;

PROTEINS;

CELL PROTEIN; PROTEIN SSGA; REGULATOR PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; GROWTH RATE; INHIBITION; MUTATION; PROTEIN; SPORULATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM MUTANT; BIOMASS; CARBOXY TERMINAL SEQUENCE; GENE LIBRARY; NONHUMAN; ORTHOLOGY; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FUNCTION; SEQUENCE ANALYSIS; SPOROGENESIS; STREPTOMYCES COELICOLOR;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; GENE LIBRARY; MOLECULAR SEQUENCE DATA; MUTATION; SPORES, BACTERIAL; STREPTOMYCETACEAE; STRUCTURE-ACTIVITY RELATIONSHIP;

ACTINOBACTERIA (CLASS); SALPIDA; STREPTOMYCES COELICOLOR; STREPTOMYCINEAE;

EID: 34247527280     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02755-06     Document Type: Article

References (31)

1. Bennett, J. W. 1998. Mycotechnology: the role of fungi in biotechnology. J. Biotechnol. 66:101-107.
2. Bentley, S. D., K. F. Chater, A. M. Cerdeno-Tarraga, G. L. Challis, N. R. Thomson, K. D. James, D. E. Harris, M. A. Quail, H. Kieser, D. Harper, A. Bateman, S. Brown, G. Chandra, C. W. Chen, M. Collins, A. Cronin, A. Fraser, A. Goble, J. Hidalgo, T. Hornsby, S. Howarth, C. H. Huang, T. Kieser, L. Larke, L. Murphy, K. Oliver, S. O'Neil, E. Rabbinowitsch, M. A. Rajandream, K. Rutherford, S. Rutter, K. Seeger, D. Saunders, S. Sharp, R. Squares, S. Squares, K. Taylor, T. Warren, A. Wietiorrek, J. Woodward, B. G. Barrell, J. Parkhill, and D. A. Hopwood. 2002. Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141-147.
3. Bushell, M. E. 1988. Actinomycetes in biotechnology, p. 185-217. Academic Press, London, United Kingdom.
4. Chater, K. F. 2001. Regulation of sporulation in Streptomyces coelicolor A3(2): a checkpoint multiplex? Curr. Opin. Microbiol. 4:667-673.
5. Chater, K. F. 1998. Taking a genetic scalpel to the streptomyces colony. Microbiology 144:1465-1478.
6. Demain, A. L. 1991. Production of beta-lactam antibiotics and its regulation. Proc. Natl. Sci. Counc. Repub. China B 15:251-265.
7. Flärdh, K., and G. P. van Wezel. 2003. Cell division during growth and development of Streptomyces, p. 71-90. In S. G. Pandalai (ed.), Recent developments in bacteriology. Transworld Research Network, Trivandrum, India.
8. Hopwood, D. A. 1999. Forty years of genetics with Streptomyces: from in vivo through in vitro to in silico. Microbiology 145:2183-2202.
9. Hopwood, D. A., K. F. Chater, and M. J. Bibb. 1995. Genetics of antibiotic production in Streptomyces coelicolor A3(2), a model streptomycete. Biotechnology 28:65-102.
10. Janssen, G. R., and M. J. Bibb. 1993. Derivatives of pUC18 that have BgIII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene 124:133-134.
11. Jiang, H., and K. E. Kendrick. 2000. Characterization of ssfR and ssgA, two genes involved in sporulation of Streptomyces griseus. J. Bacteriol. 182:5521-5529.
12. Kawamoto, S., H. Watanabe, A. Hesketh, J. C. Ensign, and K. Ochi. 1997. Expression analysis of the ssgA gene product, associated with sporulation and cell division in Streptomyces griseus. Microbiology 143:1077-1086.
13. Keijser, B. J., E. E. Noens, B. Kraal, H. K. Koerten, and G. P. van Wezel. 2003. The Streptomyces coelicolor ssgB gene is required for early stages of sporulation. FEMS Microbiol. Lett. 225:59-67.
14. Kendrick, K. E., and J. C. Ensign. 1983. Sporulation of Streptomyces griseus in submerged culture. J. Bacteriol. 155:357-366.
15. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces genetics. John Innes Foundation, Norwich, United Kingdom.
16. Larson, J. L., and C. L. Hershberger. 1986. The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid 15:199-209.
17. Lydiate, D. J., F. Malpartida, and D. A. Hopwood. 1985. The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35:223-235.
18. MacNeil, D. J., K. M. Gewain, C. L. Ruby, G. Dezeny, P. H. Gibbons, and T. MacNeil. 1992. Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector. Gene 111:61-68.
19. Minas, W., J. E. Bailey, and W. Duetz. 2000. Streptomycetes in microcultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format. Antonie Leeuwenhoek 78:297-305.
20. Noens, E. E., V. Mersinias, B. A. Traag, C. P. Smith, H. K. Koerten, and G. P. van Wezel. 2005. SsgA-like proteins determine the fate of peptidoglycan during sporulation of Streptomyces coelicolor. Mol. Microbiol. 58:929-944.
21. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
22. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Soliveri, J., A. K. Scheu, A. Hernandez, J. L. Copa-Patino, and K. F. Chater. 1999. Faster recombinant DNA procedures for Streptomyces. Bio Techniques 26:394-396.
24. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
25. Traag, B. A., G. H. Kelemen, and G. P. Van Wezel. 2004. Transcription of the sporulation gene ssgA is activated by the IclR-type regulator SsgR in a whi-independent manner in Streptomyces coelicolor A3(2). Mol. Microbiol. 53:985-1000.
26. Van Dessel, W., L. Van Mellaert, N. Geukens, and J. Anne. 2003. Improved PCR-based method for the direct screening of Streptomyces transformants. J. Microbiol. Methods 53:401-403.
27. van Wezel, G. P., P. Krabben, B. A. Traag, B. J. Keijser, R. Kerste, E. Vijgenboom, J. J. Heijnen, and B. Kraal. 2006. Unlocking Streptomyces spp. for use as sustainable industrial production platforms by morphological engineering. Appl. Environ. Microbiol. 72:5283-5288.
28. van Wezel, G. P., J. van der Meulen, S. Kawamoto, R. G. M. Luiten, H. K. Koerten, and B. Kraal. 2000. ssgA is essential for sporulation of Streptomyces coelicolor A3(2) and affects hyphal development by stimulating septum formation. J. Bacteriol. 182:5653-5662.
29. van Wezel, G. P., and E. Vijgenboom. 2004. Novel aspects of signalling in Streptomyces development. Adv. Appl. Microbiol. 56:65-88.
30. Ward, J. M., G. R. Janssen, T. Kieser, M. J. Bibb, and M. J. Buttner. 1986. Construction and characterisation of a series of multicopy promoter-probe plasmid vectors for Streptomyces using the aminoglycoside phosphotransferase gene from Tn5 as indicator. Mol. Gen. Genet 203:468-478.
31. Wardell, J. N., S. M. Stocks, C. R. Thomas, and M. E. Bushell. 2002. Decreasing the hyphal branching rate of Saccharopolyspora erythraea NRRL 2338 leads to increased resistance to breakage and increased antibiotic production. Biotechnol. Bioeng. 78:141-146.