Cytotoxicity analyses of Choukroun's platelet-rich fibrin (PRF) on a wide range of human cells: The answer to a commercial controversy

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology

Volumn 103, Issue 5, 2007, Pages 587-593

  Dohan, David M ^a   Del Corso, Marco ^b   Charrier, Jean Baptiste ^c  

^a UNIVERSITÉ PARIS DESCARTES   (France)

^b UNIVERSITY OF TURIN   (Italy)

^c UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 34247493345     PISSN: 10792104     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tripleo.2007.03.016     Document Type: Letter

References (42)

1. Choukroun J., Adda F., Schoeffler C., and Vervelle A. Une opportunité en paro-implantologie: le PRF. Implantodontie 42 (2001) 55-62
2. Dohan D.M., and Choukroun J. PRP, cPRP, PRF, PRG, PRGF, FC ... how to find your way in the jungle of platelet concentrates?. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103 (2007) 305-306
3. Dohan D.M., Choukroun J., Diss A., Dohan S.L., Dohan A.J., Mouhyi J., and Gogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101 (2006) e37-e44
4. Dohan D.M., Choukroun J., Diss A., Dohan S.L., Dohan A.J., Mouhyi J., and Gogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101 (2006) e45-e50
5. Dohan D.M., Choukroun J., Diss A., Dohan S.L., Dohan A.J., Mouhyi J., and Gogly B. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part III: leucocyte activation: a new feature for platelet concentrates?. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101 (2006) e51-e55
6. Choukroun J., Diss A., Simonpieri A., Girard M.O., Schoeffler C., Dohan S.L., et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101 (2006) e56-e60
7. Choukroun J., Diss A., Simonpieri A., Girard M.O., Schoeffler C., Dohan S.L., et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101 (2006) 299-303
8. Susini G., About I., Tran-Hung L., and Camps J. Cytotoxicity of Epiphany and Resilon with a root model. Int Endod J 39 (2006) 940-944
9. Vajrabhaya L.O., Suwannawong S.K., Kamolroongwarakul R., and Pewklieng L. Cytotoxicity evaluation of gutta-percha solvents: chloroform and GP-solvent (limonene). Oral Surg Oral Med Oral Pathol Oral Radiol Endod 98 (2004) 756-759
10. Vajrabhaya L.O., Korsuwannawong S., Jantarat J., and Korre S. Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102 (2006) e48-e50
11. Souza N.J., Justo G.Z., Oliveira C.R., Haun M., and Bincoletto C. Cytotoxicity of materials used in perforation repair tested using the V79 fibroblast cell line and the granulocyte-macrophage progenitor cells. Int Endod J 39 (2006) 40-47
12. Chen W., Hnizdo E., Chen J.Q., Attfield M.D., Gao P., Hearl F., et al. Risk of silicosis in cohorts of Chinese tin and tungsten miners, and pottery workers (I): an epidemiological study. Am J Ind Med 48 (2005) 1-9
13. Harrison J., Chen J.Q., Miller W., Chen W., Hnizdo E., Lu J., et al. Risk of silicosis in cohorts of Chinese tin and tungsten miners and pottery workers (II): workplace-specific silica particle surface composition. Am J Ind Med 48 (2005) 10-15
14. Maxim L.D., Hadley J.G., Potter R.M., and Niebo R. The role of fiber durability/biopersistence of silica-based synthetic vitreous fibers and their influence on toxicology. Regul Toxicol Pharmacol 46 (2006) 42-62
15. Elmore A.R. Final report on the safety assessment of aluminum silicate, calcium silicate, magnesium aluminum silicate, magnesium silicate, magnesium trisilicate, sodium magnesium silicate, zirconium silicate, attapulgite, bentonite, Fuller's earth, hectorite, kaolin, lithium magnesium silicate, lithium magnesium sodium silicate, montmorillonite, pyrophyllite, and zeolite. Int J Toxicol 22 Suppl 1 (2003) 37-102
16. Elmore A.R. Final report on the safety assessment of potassium silicate, sodium metasilicate, and sodium silicate. Int J Toxicol 24 Suppl 1 (2005) 103-117
17. Hnizdo E., and Vallyathan V. Chronic obstructive pulmonary disease due to occupational exposure to silica dust: a review of epidemiological and pathological evidence. Occup Environ Med 60 (2003) 237-243
18. Hnizdo E., and Murray J. Risk of pulmonary tuberculosis relative to silicosis and exposure to silica dust in South African gold miners. Occup Environ Med 55 (1998) 496-502
19. Hnizdo E., Murray J., and Klempman S. Lung cancer in relation to exposure to silica dust, silicosis and uranium production in South African gold miners. Thorax 52 (1997) 271-275
20. Murphy E.J., Roberts E., and Horrocks L.A. Aluminum silicate toxicity in cell cultures. Neuroscience 55 (1993) 597-605
21. Geurtsen W. Toxicology of dental materials and "clinical experience.". J Dent Res 82 (2003) 500
22. Geurtsen W., Spahl W., Muller K., and Leyhausen G. Aqueous extracts from dentin adhesives contain cytotoxic chemicals. J Biomed Mater Res 48 (1999) 772-777
23. Souza P.P., Aranha A.M., Hebling J., Giro E.M., and Costa C.A. In vitro cytotoxicity and in vivo biocompatibility of contemporary resin-modified glass-ionomer cements. Dent Mater 22 (2006) 838-844
24. Aranha A.M., Giro E.M., Souza P.P., Hebling J., and de Souza Costa C.A. Effect of curing regime on the cytotoxicity of resin-modified glass-ionomer lining cements applied to an odontoblast-cell line. Dent Mater 22 (2006) 864-869
25. Sipahi C., Ozen J., Ural A.U., Dalkiz M., and Beydemir B. The effect of two fibre impregnation methods on the cytotoxicity of a glass and carbon fibre-reinforced acrylic resin denture base material on oral epithelial cells and fibroblasts. J Oral Rehabil 33 (2006) 666-673
26. Karimjee C.K., Koka S., Rallis D.M., and Gound T.G. Cellular toxicity of mineral trioxide aggregate mixed with an alternative delivery vehicle. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 102 (2006) e115-e120
27. Rodriguez A.M., Elabd C., Amri E.Z., Ailhaud G., and Dani C. The human adipose tissue is a source of multipotent stem cells. Biochimie 87 (2005) 125-128
28. Rodriguez A.M., Elabd C., Delteil F., Astier J., Vernochet C., Saint-Marc P., et al. Adipocyte differentiation of multipotent cells established from human adipose tissue. Biochem Biophys Res Commun 315 (2004) 255-263
29. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65 (1983) 55-63
30. Marx R.E., Carlson E.R., Eichstaedt R.M., Schimmele S.R., Strauss J.E., and Georgeff K.R. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85 (1998) 638-646
31. Gibble J.W., and Ness P.M. Fibrin glue: the perfect operative sealant?. Transfusion 30 (1990) 741-747
32. Lawson J.H. The clinical use and immunologic impact of thrombin in surgery. Semin Thromb Hemost 32 Suppl 1 (2006) 98-110
33. Ortel T.L., Mercer M.C., Thames E.H., Moore K.D., and Lawson J.H. Immunologic impact and clinical outcomes after surgical exposure to bovine thrombin. Ann Surg 233 (2001) 88-96
34. Su Z., Izumi T., Thames E.H., Lawson J.H., and Ortel T.L. Antiphospholipid antibodies after surgical exposure to topical bovine thrombin. J Lab Clin Med 139 (2002) 349-356
35. Adams J.D., Jones S., and Brost B.C. Development of antibodies to topical bovine thrombin after abdominal hysterectomy. A case report. J Reprod Med 46 (2001) 909-912
36. Dorion R.P., Hamati H.F., Landis B., Frey C., Heydt D., and Carey D. Risk and clinical significance of developing antibodies induced by topical thrombin preparations. Arch Pathol Lab Med 122 (1998) 887-894
37. Neschis D.G., Heyman M.R., Cheanvechai V., Benjamin M.E., and Flinn W.R. Coagulopathy as a result of factor V inhibitor after exposure to bovine topical thrombin. J Vasc Surg 35 (2002) 400-402
38. Israels S.J., and Israels E.D. Development of antibodies to bovine and human factor V in two children after exposure to topical bovine thrombin. Am J Pediatr Hematol Oncol 16 (1994) 249-254
39. Cmolik B.L., Spero J.A., Magovern G.J., and Clark R.E. Redo cardiac surgery: late bleeding complications from topical thrombin-induced factor V deficiency. J Thorac Cardiovasc Surg 105 (1993) 222-227 discussion 7-8
40. Spero J.A. Bovine thrombin-induced inhibitor of factor V and bleeding risk in postoperative neurosurgical patients. Report of three cases. J Neurosurg 78 (1993) 817-820
41. Zehnder J.L., and Leung L.L. Development of antibodies to thrombin and factor V with recurrent bleeding in a patient exposed to topical bovine thrombin. Blood 76 (1990) 2011-2016
42. Schoenecker J.G., Johnson R.K., Lesher A.P., Day J.D., Love S.D., Hoffman M.R., et al. Exposure of mice to topical bovine thrombin induces systemic autoimmunity. Am J Pathol 159 (2001) 1957-1969