Identification of new centrosome proteins by autoimmune patient sera

Science in China, Series C: Life Sciences

Volumn 50, Issue 2, 2007, Pages 194-202

  Xia, Liang ^a   Li, Yan ^a   Yang, Dong ^a   Wang, LiMin ^a   He, Fang ^a   Zhou, ChunYuan ^a   Li, YongZhe ^b   Zeng, ChangQing ^a   He, DaCheng ^a  

^a BEIJING NORMAL UNIVERSITY   (China)

^b PEKING UNION MEDICAL COLLEGE HOSPITAL   (China)

Author keywords

Autoimmune patient sera; Centrosome; Elution of antibody; Immunoflourescence; Immunoprecipitation; Mass spectrometry

Indexed keywords

ANTIBODY; CYTOSKELETON PROTEIN; ENZYME;

ARTICLE; AUTOIMMUNE DISEASE; BLOOD; CENTROSOME; HELA CELL; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; PHYSIOLOGY;

ANTIBODIES; AUTOIMMUNE DISEASES; CENTROSOME; CYTOSKELETAL PROTEINS; ENZYMES; HELA CELLS; HUMANS;

EID: 34247377260     PISSN: 10069305     EISSN: 18622798     Source Type: Journal    
DOI: 10.1007/s11427-007-0014-4     Document Type: Article

References (19)

1. Doxsey S. Re-evaluating centrosome function. Nat Rev Mol Cell Biol, 2001, 2 (9): 688-698
2. Prasanth S G, Prasanth K V, Siddigui K, et al. Human Orc2 localizes to centrosomes, centromeres and heterochromatin during chromosome inheritance. EMBO J, 2004, 23: 2651-2663
3. Hori T, Haraguchi T, Hiraoka Y, et al. Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J Cell Sci, 2003, 116: 3347-3362
4. Jackman M, Lindon C, Niqq E A, et al. Active cyclin B1-Cdk1 first appears on centrosomes in prophase. Nat Cell Biol, 2003, 5: 143-148
5. Brinkley B R. Managing the centrosome numbers game: From chaos to stability in cancer cell division. Trends Cell Biol, 2001, 11: 18-21
6. Anderson J S, Wilkinson C J, Mayor T, et al. Proteomic characterization of the human centrosome by protein correlation profiling. Nature, 2003, 426 (6966): 570-574
7. Sauer G, Korner R, Hanisch A, et al. Proteome analysis of the human mitotic spindle. Mol Cell Proteomics, 2005, 4 (1): 35-43
8. Moroi Y, Peebles C, Fritzler M J, et al. Autoantibody to centromere (kinetochore) in scleroderma sera. Proc Natl Acad Sci USA, 1980, 77: 1627-1631
9. Earnshaw W C, Rothfield N. Identification of a family of human centromere proteins using autoimmune sera from patients with scleroderma. Chromosoma, 1985, 91: 313-321
10. Craig J M, Earnshaw W C, Vagnarelli P. Mammalian centromeres: DNA sequence, protein composition, and role in cell cycle progression. Exp Cell Res, 1999, 246: 249-262
11. He D, Zeng C, Woods K, et al. CENP-G: A new centromeric protein that is associated with the a-1 satellite DNA subfamily. Chromosoma, 1998, 107: 189-197
12. Guo D, Hu K, Lei Y, et al. Identification and characterization of a novel cytoplasm protein ICF45 that is involved in cell cycle regulation. J Biol Chem, 2004, 279 (51): 53498-53505
13. Olmsted J B. Affinity purification of antibodies from diazotized paper blots of heterogeneous protein samples. J Biol Chem, 1981, 256 (23): 11955-11957
14. Leeb-Lundberg L M, Song X H. Identification of P125, A component of a group of 120-kDa proteins that are phosphorylated on tyrosine residues in response to bradykinin and bombesin stimulation, in anti-ras-GTPase-activiting protein immunoprecipitates of Swiss 3T3 cells. J Biol Chem, 1993, 268 (11): 8151-8157
15. Shevchenko A, Wilm M, Vorm O, et al. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gel. Anal Chem, 1996, 68: 850-858
16. Bjellqvist B, Pasquali C, Ravier F, et al. A nonlinear wide-range immobilized pH gradient for two-dimentional electrophoresis and its definition in a relevant pH scale. Electrophoresis, 1993, 14: 1357-1365
17. Gharahdaghi F, Weinberg C R, Meagher D A, et al. Mass spectrometric identification of proteins from silver-stained polyacrylamide gel: A method for the removal of silver ions to enhance sensitivity. Electrophoresis, 1999, 20: 601-605
18. Ikegaki N, Tang X X, Liu X G, et al. Molecular characterization and chromosomal localization of DRT(EPHT3): A developmentally regulated human protein-tyrosine kinase gene of the EPH family. Hum Mol Genet, 1995, 4 (11): 2033-2045
19. Lange B M, Bachi A, William M, et al. Hsp90 is a core centrosomal component and is required at different stages of the centrosome cycle in Drosophila and vertebrates. EMBO J, 2000, 19: 1252-1262