A simple, computer-assisted method for obtaining and counting hemocytes in milkweed bugs: Technical note

Entomologia Experimentalis et Applicata

Volumn 123, Issue 2, 2007, Pages 203-206

  Davis, Andrew K ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

Hemocyte counts; Homoptera; Image analysis; Lygaeidae; Oncopeltus fasciatus

Indexed keywords

COMPUTER AIDED DESIGN; IMAGE ANALYSIS; INSECT; MEASUREMENT METHOD;

HEMIPTERA; LYGAEIDAE; ONCOPELTUS FASCIATUS;

EID: 34247340902     PISSN: 00138703     EISSN: 15707458     Source Type: Journal    
DOI: 10.1111/j.1570-7458.2007.00540.x     Document Type: Article

References (15)

1. Andrade FG, Negreiro MCC, Cortez MM, Silva VB & Falleiros AMF (2003) Total and differential counting of the hemocytes in Anticarsia gemmatalis (Lepidoptera: Noctuidae) larvae inoculated with Baculovirus anticarsia. Acta Microscopica 12 (Suppl. B): 407-408.
2. Armitage SAO & Siva-Jothy MT (2005) Immune function responds to selection for cuticular colour in Tenebrio molitor. Heredity 94: 650-656.
3. Clark ME & Jones JC (1980) Hematocrit method for determining total hemocyte counts of larvae of the tobacco budworm, Heliothis virescens (Lepidoptera, Noctuidae). Annals of the Entomological Society of America 73: 683-685.
4. Davis AK, Altizer SM & Friedle E (2004) A non-destructive, automated method of counting spores of Ophryocystis elektroscirrha in infected monarch butterflies (Lepidoptera: Nymphalidae). Florida Entomologist 87: 231-234.
5. Feir D (1964) Haemocyte counts on the large milkweed bug, Oncopeltus fasciatus. Nature 202: 1136-1137.
6. Feir D (1974) Oncopeltus fasciatus - research animal. Annual Review of Entomology 19: 81-96.
7. Feir D & O'Connor GM (1969) Liquid nitrogen fixation: a new method for hemocyte counts and mitotic indices in tissue sections. Annals of the Entomological Society of America 62: 246-247.
8. Hall DW (1983) Mosquito hemocytes - a review. Developmental and Comparative Immunology 7: 1-12.
9. Jones JC (1962) Current concepts concerning insect hemocytes. American Zoologist 2: 209-246.
10. Mochiah MB, Ngi-song AJ, Overholt WA & Botchey M (2003) Variation in total and differential haemocyte count of Busseola fusca (Lepidoptera: Noctuidae) parasitized by two biotypes of Cotesia sesamiae (Hymenoptera: Braconidae) and larval growth responses. Environmental Entomology 32: 247-255.
11. Ravindranath MH (1978) Individuality of plasmatocytes and granular hemocytes of arthropods - review. Developmental and Comparative Immunology 2: 581-594.
12. Ribeiro C & Brehelin M (2006) Insect haemocytes: what type of cell is that? Journal of Insect Physiology 52: 417-429.
13. Rolff J & Siva-Jothy MT (2003) Invertebrate ecological immunity. Science 301: 472-475.
14. Silva JEB, Boleli IC & Simoes ZLP (2002) Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Brazilian Journal of Biology 62: 689-699.
15. Stewart JE, Cornick JW & Dingle JR (1967) An electronic method for counting lobster (Homarus americanus Milne Edwards) hemocytes and the influence of diet on hemocyte numbers and hemolymph proteins. Canadian Journal of Zoology 45: 291-304.